The Saccharomyces cerevisiae RAD9 cell cycle checkpoint gene is required for optimal repair of UV-induced pyrimidine dimers in both G1 and G2/M phases of the cell cycle
Open accessAbstract
Cells respond to DNA damage by activating both cellular growth arrest and DNA repair processes. In Saccharomyces cerevesiae the RAD9 gene controls DNA damage-mediated cell cycle arrest that is known to allow efficient repair. To ascertain whether RAD9 plays a role in DNA repair per se, the removal of UV-induced photolesions was assessed in synchronized isogenic normal and rad9 cells using the high resolution primer extension technique. The results show that RAD9 is indeed involved in the removal of photolesions from both the transcribed and the non-transcribed strands of the reporter GAL10 gene, in G(1)- as well as G(2)/M-arrested cells. Interestingly, these data also reveal that in both normal and rad9 mutant, the repair strand bias towards the transcribed stand is more pronounced in G(2)/M- than in G(1)-arrested cells. These data indicate that RAD9 coordinate the cellular response to DNA damage by activating both cell cycle checkpoint and excision repair.
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S. cerevisiae Rad9 plays a role in repair of both UV-damaged strands of an actively transcribed gene, likely indirectly through up-regulation of NER genes RAD2, RAD7, RAD16, and RAD23.
“The S. cerevisiae Rad9 gene product plays a role in the repair of both UV-damaged strands of an actively transcribed gene; this effect on NER is likely indirect and probably occurs through up-regulation of some NER genes (i.e., RAD2, RAD7, RAD16 and RAD23), consistently with a previously reported Rad9-dependent stimulation of NER genes transcription [98].”
From Mind the gap: keeping UV lesions in check (2011) · cites this paper as [98]
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Mind the gap: keeping UV lesions in check
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Self-citation
- Authors
- Al-Moghrabi, N. M.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-05-15
- Volume
- 29
- Issue
- 10
- Pages
- 2020-2025
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC55462
- OpenAlex
- W2071941877
- Cited by
- 24
- References
- 27
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 1 · 2019: 2 · 2016: 1 · 2014: 1 · 2013: 2
- FWCI
- 0.58
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Nucleic Acids Research (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Rad9, cell cycle checkpoints, DNA repair, G1 cell cycle arrest, G2/M cell cycle arrest, excision repair, strand bias, DNA damage response, Saccharomyces cerevisiae
- MeSH terms
- Mating Factor, Cell Cycle, DNA Damage, DNA Repair, Genes, Fungal, Mitosis, Peptides, Pyrimidine Dimers, Saccharomyces cerevisiae, Transcription, Genetic, Ultraviolet Rays, G1 Phase, G2 Phase, Gene Deletion, Genes, Reporter, Cell Cycle Proteins
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