The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
IL-1 induces collagenase-3 (MMP-13) promoter activity in stably transfected chondrocytic cells: requirement for Runx-2 and activation by p38 MAPK and JNK pathways
Open accessAbstract
Osteoarthritic chondrocytes secrete matrix metalloproteinase-13 (MMP-13) in response to interleukin-1 (IL-1), causing digestion of type II collagen in cartilage. Using chondrocytic cells, we previously determined that IL-1 induced a strong MMP-13 transcriptional response that requires p38 MAPK, JNK and the transcription factor NF-kappaB. Now, we have studied the tissue-specific transcriptional regulation of MMP-13. Constitutive expression of the transcription factor Runx-2 correlated with the ability of a cell type to express MMP-13 and was required for IL-1 induction; moreover, Runx-2 enhanced IL-1 induction of MMP-13 transcription by synergizing with the p38 MAPK signaling pathway. Transiently transfected MMP-13 promoters were not IL-1 inducible. However, -405 bp of stably integrated promoter was sufficient for 5- to 6-fold IL-1 induction of reporter activity and this integrated reporter required the same p38 MAPK pathway as the endogenous gene. Finally, mutation of the proximal Runx binding site and the proximal AP-1 site blunted the transcriptional response to IL-1, and double mutation synergistically decreased reporter activity. In summary, our data suggest that the transcriptional MMP-13 response to IL-1 is controlled by the p38 pathway interacting at the MMP-13 promoter through the tissue-specific transcription factor Runx-2 and the ubiquitous AP-1 transcription factor.
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Cited by
Luo, L., Li, D.-Q., Doshi, A., Farley, W., Corrales, R. M., & Pflugfelder, S. C. (2004) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.03-1145 · Cited by 595
The regulation of matrix metalloproteinases and their inhibitors
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Hyperosmolar Saline Is a Proinflammatory Stress on the Mouse Ocular Surface
Luo, L., Li, D.-Q., Corrales, R. M., & Pflugfelder, S. C. (2005) · Eye & Contact Lens: Science & Clinical Practice · doi:10.1097/01.icl.0000162759.79740.46 · Cited by 340
Metadata
- Authors
- Mengshol, J. A.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-11-01
- Volume
- 29
- Issue
- 21
- Pages
- 4361-4372
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2167094540
- Cited by
- 256
- References
- 35
- FWCI
- 6.94
- Type
- journal article
- Language
- en
- Open access
- green
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- Mengshol, J. A. (2001). IL-1 induces collagenase-3 (MMP-13) promoter activity in stably transfected chondrocytic cells: requirement for Runx-2 and activation by p38 MAPK and JNK pathways. Nucleic Acids Research. https://doi.org/10.1093/nar/29.21.4361
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