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A simple method for DNA restriction site mapping

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Smith, H. O., Birnstiel, M. L.·1976·Nucleic Acids Research

Abstract

When a DNA molecule, enzymatically labelled with 32p at one end, is partially digested with a restriction enzyme labelled tdna fragments are obtained which form an overlapping series of molecules, all with a common labelled terminus. ta restriction map can then be constructed from an analysis of the size distribution of these molecules. This technique has been used for the restriction site mapping of cloned histone DNA (h22) where as many as 35 cleavage sites may be accurately determined in a single experiment.

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Metadata

Authors
Smith, H. O., & Birnstiel, M. L.
Venue
Nucleic Acids Research
Published
1976-09-01
Volume
3
Issue
9
Pages
2387-2398
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W1984917385
Cited by
1,010
References
0
FWCI
19.43
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: A simple method for DNA restriction site mapping. https://app.cochat.ai/papers/10.1093/nar/3.9.2387
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Smith, H. O., & Birnstiel, M. L. (1976). A simple method for DNA restriction site mapping. Nucleic Acids Research. https://doi.org/10.1093/nar/3.9.2387

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