The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Quantitative reversed-phase high performance liquid chromatographic determination of major and modified deoxyribonucleosides in DNA
Open accessAbstract
We have developed a method to accurately determine (< 3% RSD) the complete major and modified base composition of a few micrograms of unlabeled DNA. The DNA samples were quantitatively hydrolyzed with DNase 1, Nuclease P1, and bacterial alkaline phosphatase. The resulting deoxyribonucleosides were directly separated in 70 min by reversed-phase high performance liquid chromatography with detection by ultraviolet absorption at 254 nm and 280 nm (RP-HPLC). The highly sensitive and selective dual wavelength quantitation greatly enhances the precision and accuracy of the chromatographic analysis. Contamination of DNA preparations with RNA does not interfere with the DNA analysis due to the high resolution of the chromatography. We have used this method for the quantitation of m5dCyd in 5 microgram of calf thymus and salmon sperm DNA in which the m5dCyd comprises only 1 to 2% of the total bases. This method should be a useful research tool in studies on various DNAs and DNA subfractions and should help to elucidate the functions of methylation of DNA.
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Cites
A simple method for DNA restriction site mapping
Smith, H. O., & Birnstiel, M. L. (1976) · Nucleic Acids Research · doi:10.1093/nar/3.9.2387 · Cited by 1,010
Distribution of 5-methylcytosine in chromatin.
Razin, A., & Cedar, H. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.7.2725 · Cited by 205
Lapeyre, J.-N., & Becker, F. F. (1979) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(79)92015-1 · Cited by 180
The content of 5-methylcytosine in animal DNA: The species and tissue specificity
Vanyushin, B. F., Mazin, A. L., Vasilyev, V. K., et al. (1973) · Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis · doi:10.1016/0005-2787(73)90264-5 · Cited by 156
Methylated bases in DNA from Paramecium aurelia
Cummings, D. J., Tait, A., & Goddard, J. M. (1974) · Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis · doi:10.1016/0005-2787(74)90194-4 · Cited by 140
Rae, P. M. M., & Steele, R. E. (1978) · Biosystems · doi:10.1016/0303-2647(78)90027-8 · Cited by 110
Mouse DNA methylase: Methylation of native DNA
Adams, R. L. P., McKay, E. L., Craig, L., & Burdon, R. H. (1979) · Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis · doi:10.1016/0005-2787(79)90143-6 · Cited by 85
Wakizaka, A., Kurosaka, K., & Okuhara, E. (1979) · Journal of Chromatography B Biomedical Sciences and Applications · doi:10.1016/s0378-4347(00)81518-2 · Cited by 48
Breter, H.-J., & Zahn, R. K. (1973) · Analytical Biochemistry · doi:10.1016/0003-2697(73)90362-x · Cited by 22
Cited by
DNMT1 and DNMT3b cooperate to silence genes in human cancer cells
Rhee, I., Bachman, K. E., Park, B. H., Jair, K.-W., Yen, R.-W. C., Schuebel, K. E., Cui, H., Feinberg, A. P., Lengauer, C., Kinzler, K. W., et al. (2002) · Nature · doi:10.1038/416552a · Cited by 1,039
Metadata
- Authors
- Kuo, K. C., McCune, R. A., Gehrke, C. W., Midgett, R., & Ehrlich, M.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1980
- Volume
- 8
- Issue
- 20
- Pages
- 4763-4776
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2001425587
- Cited by
- 329
- References
- 9
- FWCI
- 5.75
- Type
- journal article
- Language
- en
- Open access
- green
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- Kuo, K. C., McCune, R. A., Gehrke, C. W., Midgett, R., & Ehrlich, M. (1980). Quantitative reversed-phase high performance liquid chromatographic determination of major and modified deoxyribonucleosides in DNA. Nucleic Acids Research. https://doi.org/10.1093/nar/8.20.4763
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
