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The use of nuclease P1in sequence analysis of end group labeled RNA
Open accessAbstract
A method is described for the direct sequence analysis of 20-25 nucleotides from the termini of 5'- or 3'-end-group [32P] labeled RNA. The method involves partial endonucleolytic digestion of the labeled RNA with nuclease P1 (from Penicillium citrinum) followed by separation of the partial digestion products by two-dimensional homochromatography, the nucleotide sequence being determined by mobility shift analysis. This procedure has been applied to the sequence analysis of the terminal regions of tRNAs and of high molecular weight RNA, such as messenger RNA or viral RNA. A further application involves its use in conjunction with snake venom phosphodiesterase to determine the sequence of 5'-end group labeled oligonucleotides, containing modified bases, derived from T1 or pancreatic RNase digestion of tRNA.
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Citations
Cited by
Kozak, M. (1981) · Nucleic Acids Research · doi:10.1093/nar/9.20.5233 · Cited by 1,242
Probing the structure of RNAs in solution
Ehresmann, C., Baudin, F., Mougel, M., Romby, P., Ebel, J.-P., & Ehresmann, B. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.22.9109 · Cited by 730
Takeshita, M., Chang, C. N., Johnson, F., Will, S., & Grollman, A. P. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)61093-2 · Cited by 400
Ohtsuka, E., Matsuki, S., Ikehara, M., Takahashi, Y., & Matsubara, K. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)89400-5 · Cited by 302
The RNA required in the first step of chlorophyll biosynthesis is a chloroplast glutamate tRNA
Schön, A., Krupp, G., Gough, S., Berry-Lowe, S., Kannangara, C. G., & Söll, D. (1986) · Nature · doi:10.1038/322281a0 · Cited by 301
Metadata
- Authors
- Silberklang, M., Gillum, A. M., & Rajbhandary, U. L.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1977
- Volume
- 4
- Issue
- 12
- Pages
- 4091-4108
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2040179169
- Cited by
- 238
- References
- 0
- FWCI
- 8.99
- Type
- journal article
- Language
- en
- Open access
- green
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- Silberklang, M., Gillum, A. M., & Rajbhandary, U. L. (1977). The use of nuclease P1in sequence analysis of end group labeled RNA. Nucleic Acids Research. https://doi.org/10.1093/nar/4.12.4091
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