The DOI matches its Crossref record.
Mapping adenines, guanines, and pyrimidines in RNA
Open accessAbstract
The positions of adenines, guanines, and pyrimidines can be determined by partial nuclease digestion of a terminally labeles RNA molecule. In urea, at elevated temperatures, RNase T1 generates a pattern reflecting cleavage at guanines while RNase U2 cleaves only at adenine. A limited alkaline hydrolysis provides a continuum of fragments derived from breaks at every phosphodiester bond. The reaction products are electrophoretically fractionated by size in adjacent lanes of a polyacrylamide gel. An autoradiograph of the gel displays the sequence up to 100 nucleotides from the end of the molecule, although uracil cannot as yet be distinguished from cytosine. These techniques form the basis of an RNA sequencing method and are demonstrated on yeast 5.8S ribosomal RNA.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Kruger, K., Grabowski, P. J., Zaug, A. J., Sands, J., Gottschling, D. E., & Cech, T. R. (1982) · Cell · doi:10.1016/0092-8674(82)90414-7 · Cited by 2,331
Direct chemical method for sequencing RNA.
Peattie, D. A. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.4.1760 · Cited by 940
3′-Terminal labelling of RNA with T4 RNA ligase
ENGLAND, T. E., & UHLENBECK, O. C. (1978) · Nature · doi:10.1038/275560a0 · Cited by 555
Sequence requirements of the hammerhead RNA self-cleavage reaction
Ruffner, D. E., Stormo, G. D., & Uhlenbeck, O. C. (1990) · Biochemistry · doi:10.1021/bi00499a018 · Cited by 470
[67] A molecular titration assay to measure transcript prevalence levels
Lee, J. J., & Costlow, N. A. (1987) · Methods in Enzymology · doi:10.1016/0076-6879(87)52070-5 · Cited by 149
Metadata
- Authors
- Donis-Keller, H., Maxam, A. M., & Gilbert, W.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1977
- Volume
- 4
- Issue
- 8
- Pages
- 2527-2538
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1970667469
- Cited by
- 1,371
- References
- 0
- FWCI
- 42.50
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Mapping adenines, guanines, and pyrimidines in RNA. https://app.cochat.ai/papers/10.1093/nar/4.8.2527
- The paper
- Donis-Keller, H., Maxam, A. M., & Gilbert, W. (1977). Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Research. https://doi.org/10.1093/nar/4.8.2527
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
