[67] A molecular titration assay to measure transcript prevalence levels
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The probe excess titration method is extremely sensitive, permitting reliable detection of <0.05 mRNA molecules per average embryonic cell.
“it is extremely sensitive, permitting reliable detection of <0.05 mRNA molecules per average embryonic cell”
From Expression of the <i>Hox</i> gene complex in the indirect development of a sea urchin (1998) · cites this paper as [20]
Cites
Mapping adenines, guanines, and pyrimidines in RNA
Donis-Keller, H., Maxam, A. M., & Gilbert, W. (1977) · Nucleic Acids Research · doi:10.1093/nar/4.8.2527 · Cited by 1,380 · see citing works
Hybridization of nucleic acids immobilized on solid supports
Meinkoth, J. L., & Wahl, G. M. (1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90808-x · Cited by 1,351 · see citing works
Assay for nanogram quantities of DNA in cellular homogenates
Brunk, C. F., Jones, K. C., & James, T. W. (1979) · Analytical Biochemistry · doi:10.1016/0003-2697(79)90690-0 · Cited by 904 · see citing works
Casey, J. W., & Davidson, N. (1977) · Nucleic Acids Research · doi:10.1093/nar/4.5.1539 · Cited by 713 · see citing works
The making of strand-specific M13 probes
Hu, N.-T., & Messing, J. W. (1982) · Gene · doi:10.1016/0378-1119(82)90143-3 · Cited by 649 · see citing works
Human β-globin pre-mRNA synthesized in vitro is accurately spliced in xenopus oocyte nuclei
Green, M. R., Maniatis, T., & Melton, D. A. (1983) · Cell · doi:10.1016/0092-8674(83)90054-5 · Cited by 474 · see citing works
Nucleic acid hybridization: A practical approach
Flavell, A. J. (1987) · Clinica Chimica Acta · doi:10.1016/0009-8981(87)90057-x · Cited by 406 · see citing works
Activation of sea urchin actin genes during embryogenesis
Lee, J. J., Calzone, F. J., Britten, R. J., Angerer, R. C., & Davidson, E. H. (1986) · Journal of Molecular Biology · doi:10.1016/0022-2836(86)90302-5 · Cited by 131 · see citing works
Self-citationWilliams, J. G., & Lloyd, M. M. (1979) · Journal of Molecular Biology · doi:10.1016/0022-2836(79)90056-1 · Cited by 105 · see citing works
Specific representation of cloned repetitive DNA sequences in sea urchin RNAs
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Construction of a cloned library of expressed embryonic gene sequences from Xenopus laevis
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BDNF mRNA is decreased in the hippocampus of individuals with Alzheimer's disease
Phillips, H. S., Hains, J. M., Armanini, M., Laramee, G. R., Johnson, S. A., & Winslow, J. W. (1991) · Neuron · doi:10.1016/0896-6273(91)90273-3 · Cited by 921 · see citing works
Nicotine binding and nicotinic receptor subunit RNA after chronic nicotine treatment
Marks, M., Pauly, Jr., Gross, S., Deneris, E., Hermans-Borgmeyer, I., Heinemann, S., & Collins, A. (1992) · The Journal of Neuroscience · doi:10.1523/jneurosci.12-07-02765.1992 · Cited by 551 · see citing works
Expression of the <i>Hox</i> gene complex in the indirect development of a sea urchin
Arenas-Mena, C., Martinez, P., Cameron, R. A., & Davidson, E. H. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.22.13062 · Cited by 137 · see citing works
Peterson, K. J., Irvine, S. Q., Cameron, R. A., & Davidson, E. H. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.9.4487 · Cited by 47 · see citing works
- Authors
- Lee, J. J., & Costlow, N. A.
- ORCID
- Lee, James J.
- Venue
- Methods in Enzymology
- Publisher
- Elsevier
- Published
- 1987
- Volume
- 152
- Pages
- 633-648
- ISSN
- 0076-6879 (ISSN-L)
- OpenAlex
- W126023894
- Cited by
- 149
- References
- 12
- Citations by year
- 2017: 1 · 2013: 1
- FWCI
- 5.47
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Methods in enzymology on CD-ROM/Methods in enzymology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Cancer Society
- National Institutes of Health
- National Institute of General Medical Sciences (GM07616)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD05753)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- RNA quantification, gene expression quantification
- MeSH terms
- Animals, Cloning, Molecular, Ribonuclease T1, Indicators and Reagents, Nucleic Acid Hybridization, RNA, Transcription, Genetic
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
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