A rapid alkaline extraction procedure for screening recombinant plasmid DNA

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Items 101–200 of 1,027

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2. Cloning of the Alcaligenes eutrophus genes for synthesis of poly-beta-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli
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3. The complete nucleotide sequence of theRattus norvegicus mitochondrial genome: Cryptic signals revealed by comparative analysis between vertebrates
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4. Insulin-like growth factor-I is an essential regulator of the differentiation of 3T3-L1 adipocytes.
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5. Cloning and Characterization of the Gene for β-Tubulin from a Benomyl-Resistant Mutant of Neurospora crassa and Its Use as a Dominant Selectable Marker
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6. Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes
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7. Hypomethylation of ras oncogenes in primary human cancers
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8. A sensitive genetic assay for the detection of cytosine deamination: determination of rate constants and the activation energy
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9. The receptor for transepithelial transport of IgA and IgM contains multiple immunoglobulin-like domains
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10. Primary structure and subunit stoichiometry of F1-ATPase from bovine mitochondria
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11. Illnesses Associated with Escherichia coli 0157:H7 Infections
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12. The product of the yeast UPF1 gene is required for rapid turnover of mRNAs containing a premature translational termination codon.
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13. Chimeric genes as dominant selectable markers in plant cells
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14. Species-Specific and Ubiquitous-DNA-Based Assays for Rapid Identification ofStaphylococcus aureus
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15. Multiple Antibiotic–Resistant Klebsiella and Escherichia coli in Nursing Homes
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16. Carboxy-terminal phenylalanine is essential for the correct assembly of a bacterial outer membrane protein
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17. Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis
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18. Human β-globin pre-mRNA synthesized in vitro is accurately spliced in xenopus oocyte nuclei
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19. Packaging capacity and stability of human adenovirus type 5 vectors
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20. The Diarrheal Response of Humans to Some Classic Serotypes of Enteropathogenic Escherichia coli is Dependent on a Plasmid Encoding an Enteroadhesiveness Factor
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21. Mapping of deletions in the genome of the highly attenuated vaccinia virus MVA and their influence on virulence
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22. Poly-β-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16
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23. Hospital-acquired Infection with Vancomycin-resistant Enterococcus faecium Transmitted by Electronic Thermometers
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24. White collar‐1, a central regulator of blue light responses in Neurospora, is a zinc finger protein.
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25. Hormone-mediated repression: a negative glucocorticoid response element from the bovine prolactin gene.
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26. Multimerization of high copy number plasmids causes instability: Cole 1 encodes a determinant essential for plasmid monomerization and stability
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27. Specific cloning of DNA fragments absent from the DNA of a male patient with an X chromosome deletion.
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28. The zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons
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29. Regulation of ribosomal RNA promoters with a synthetic lac operator.
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30. The barley stem rust-resistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases
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31. Fine Structure Genetic Analysis of a β-Globin Promoter
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32. Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms
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33. Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes
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34. A molecular map of the human major histocompatibility complex class III region linking complement genes C4, C2 and factor B
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35. Listeriolysin O is essential for virulence of Listeria monocytogenes: direct evidence obtained by gene complementation
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37. Phylogenetic Analysis of Aquaspirillum magnetotacticum Using Polymerase Chain Reaction-Amplified 16S rRNA-Specific DNA
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38. Genome linking with yeast artificial chromosomes
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39. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs bytrans-complementation ofSchizosaccharomyces pombe
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40. Herpes simplex virus type 1 ICP27 is an essential regulatory protein
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41. The genetic and transcriptional organization of the vir region of the A6 Ti plasmid of Agrobacterium tumefaciens.
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42. Plasmid vectors for the selection of promoters
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43. Antisense Promoter of Human L1 Retrotransposon Drives Transcription of Adjacent Cellular Genes
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44. The Yeast Cell Cycle Gene CDC34 Encodes a Ubiquitin-Conjugating Enzyme
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45. The structural basis of the multiple forms of human complement component C4
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46. pIJ101, a multi-copy broad host-range Streptomyces plasmid: Functional analysis and development of DNA cloning vectors
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47. Global and local genome mapping in Arabidopsis thaliana by using recombinant inbred lines and random amplified polymorphic DNAs.
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48. A Waterborne Outbreak in Missouri of Escherichia coli O157:H7 Associated with Bloody Diarrhea and Death
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49. The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids — a review
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50. A Tn3 lacZ transposon for the random generation of beta‐galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium.
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51. Isolation and expression of an altered mouse dihydrofolate reductase cDNA.
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52. Identification and Comparison of Stable and Unstable mRNAs in Saccharomyces cerevisiae
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53. Complementary DNA and protein sequences of ethanol-inducible rat and human cytochrome P-450s. Transcriptional and post-transcriptional regulation of the rat enzyme.
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54. Molecular Cloning of the Human XRCC1 Gene, Which Corrects Defective DNA Strand Break Repair and Sister Chromatid Exchange
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55. Nucleotide sequence analysis of TL-DNA of Agrobacterium rhizogenes agropine type plasmid. Identification of open reading frames.
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56. Clusters of genes encoding mouse transplantation antigens
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57. Discoordinate expression of stromelysin, collagenase, and tissue inhibitor of metalloproteinases-1 in rheumatoid human synovial fibroblasts. Synergistic effects of interleukin-1 and tumor necrosis factor-alpha on stromelysin expression.
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58. A mutant insulin receptor with defective tyrosine kinase displays no biologic activity and does not undergo endocytosis.
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59. RFLP analysis and linkage mapping in Solanum tuberosum
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60. A nonsecretable cell surface mutant of tumor necrosis factor (TNF) kills by cell-to-cell contact
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61. Construction and characterisation of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator
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62. Genetic and structural characterization of the avirulence gene avrBs3 from Xanthomonas campestris pv. vesicatoria
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63. Drug-Resistant Salmonella from Animals Fed Antimicrobials
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64. Construction of a cloning site near one end of Tn917 into which foreign DNA may be inserted without affecting transposition in Bacillus subtilis or expression of the transposon-borne erm gene
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65. ‘ATG vectors’ for regulated high-level expression of cloned genes in Escherichia coli
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66. Sequence-specific recognition of RNA hairpins by bacteriophage antiterminators requires a conserved arginine-rich motif
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67. Rapid and easy sequencing of large linear double-stranded DNA and supercoiled plasmid DNA
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68. Agrobacterium-mediated delivery of infectious maize streak virus into maize plants
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69. Pseudomonas aeruginosa lectin LecB is located in the outer membrane and is involved in biofilm formation
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70. Effects of Ionizing Radiation on a Plant Genome: Analysis of Two Arabidopsis transparent testa Mutations
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71. Characterization of the phosphoenolpyruvate carboxykinase (GTP) promoter-regulatory region. II. Identification of cAMP and glucocorticoid regulatory domains.
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72. The mouse glucocorticoid receptor: mapping of functional domains by cloning, sequencing and expression of wild‐type and mutant receptor proteins.
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73. A Comparative Study of Different Methods of Yeast Strain Characterization
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74. Complete sequence of the cDNA for human .alpha.1-antitrypsin and the gene for the S variant
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75. Effect of Subinhibitory Antibiotic Concentrations on Polysaccharide Intercellular Adhesin Expression in Biofilm-Forming Staphylococcus epidermidis
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76. Mitochondrial DNA polymorphism in a maternal lineage of Holstein cows.
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77. Kinetic Characterization of Individual Hexose Transporters of Saccharomyces Cerevisiae and their Relation to the Triggering Mechanisms of Glucose Repression
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78. Cloning and sequence of a cDNA coding for the human beta-migrating endothelial-cell-type plasminogen activator inhibitor.
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79. Transformation of rat cells by an altered polyoma virus genome expressing only the middle-T protein
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80. Isolation and structural analysis of chloroplast DNA
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81. Uncoupling of the cytochrome P-450cam monooxygenase reaction by a single mutation, threonine-252 to alanine or valine: possible role of the hydroxy amino acid in oxygen activation.
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82. Efflux-mediated multidrug resistance in Bacillus subtilis: similarities and dissimilarities with the mammalian system.
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83. Mapping of steroid 21-hydroxylase genes adjacent to complement component C4 genes in HLA, the major histocompatibility complex in man.
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84. Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics
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85. Adult forms of the Ca2+ATPase of sarcoplasmic reticulum. Expression in developing skeletal muscle.
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86. Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene from Thermus aquaticus
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87. Staphylococcal protein A consists of five IgG-binding domains
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88. EndoS, a novel secreted protein from Streptococcus pyogenes with endoglycosidase activity on human IgG
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89. Virulence genes regulated at the transcriptional level by Ca2+ in Yersinia pestis include structural genes for outer membrane proteins
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91. Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase II transcripts
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92. Effects of Enterococcus faecalis fsr Genes on Production of Gelatinase and a Serine Protease and Virulence
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93. Transfer of multiple drug resistance plasmids between bacteria of diverse origins in natural microenvironments
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94. E.coli Fis protein activates ribosomal RNA transcription in vitro and in vivo.
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95. Embryonic ε and γ globin genes of a prosimian primate (Galago crassicaudatus)
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96. Root lectin as a determinant of host–plant specificity in the Rhizobium–legume symbiosis
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97. The dhb Operon of Bacillus subtilisEncodes the Biosynthetic Template for the Catecholic Siderophore 2,3-Dihydroxybenzoate-Glycine-Threonine Trimeric Ester Bacillibactin
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98. Identification and Characterization of a Gene Cluster for Synthesis of the Polyketide Antibiotic 2,4-Diacetylphloroglucinol from Pseudomonas fluorescens Q2-87
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99. Detection of Human Cytomegalovirus in Peripheral Blood Lymphocytes in a Natural Infection
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100. Hormone phage: An enrichment method for variant proteins with altered binding properties
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