Cited by 14,686Molecular Biology Techniques and ApplicationsBacterial Genetics and BiotechnologyDNA and Nucleic Acid Chemistry

The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

Open access

Birnboim, H.C., Doly, J.·1979·Nucleic Acids Research

DOI 10.1093/nar/7.6.1513PMC PMC342324

Open this paper in CoChat — free account

Abstract

A procedure for extracting plasmid DNA from bacterial cells 1s described. The method 1s simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circular DNA remains double-stranded. Adequate pH control is accomplished without using a pH meter. Upon neutralization, chromosomal DNA renatures to form an insoluble clot, leaving plasmid DNA in the supernatant. Large and small plasmid DNAs have been extracted by this method.

Integrity signals

Registry

Crossref · 10.1093/nar/7.6.1513

Cites

20 of 20 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.
Bolívar, F. G., Rl, R., Pj, G., Betlach, M. C., Hl, H., Boyer, H. W., Crosa, J. H., & Falkow, S. (1992) · PubMed · Cited by 4,672
A complementation analysis of the restriction and modification of DNA in Escherichia coli
Boyer, H. W., & Roulland-Dussoix, D. (1969) · Journal of Molecular Biology · doi:10.1016/0022-2836(69)90288-5 · Cited by 3,768
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Bolivar, F., Rodriguez, R. L., Greene, P. J., Betlach, M. C., Heyneker, H. L., Boyer, H. W., Crosa, J. H., & Falkow, S. (1977) · PubMed · Cited by 2,750
SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN Escherichia coli : PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM
Clewell, D. B., & Helinski, D. R. (1969) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.62.4.1159 · Cited by 1,830
Nature of Col E 1 Plasmid Replication in Escherichia coli in the Presence of Chloramphenicol
Clewell, D. B. (1972) · Journal of Bacteriology · doi:10.1128/jb.110.2.667-676.1972 · Cited by 1,193
A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria
Eckhardt, T. (1978) · Plasmid · doi:10.1016/0147-619x(78)90016-1 · Cited by 945
THE PREPARATION AND CHARACTERIZATION OF RIBONUCLEIC ACIDS FROM YEAST
Crestfield, A. M., Smith, K. C., & Allen, F. W. (1955) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52295-5 · Cited by 440
Isolation of covalently closed circular DNA of high molecular weight from bacteria
Currier, T. C., & Nester, E. W. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90338-9 · Cited by 408
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli
Sharp, P. A., Hsu, M., Ohtsubo, E., & Davidson, N. (1972) · Journal of Molecular Biology · doi:10.1016/0022-2836(72)90363-4 · Cited by 380
Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads.
Collins, J. F., & Höhn, B. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.9.4242 · Cited by 360

Flagged first, then by citations

Show all 20 references

Sorted by citations

Items 801–900 of 1,027

Mutational Analysis of the Consensus Sequence of a Replication Origin from Yeast Chromosome III
van Houten, J. V., & Newlon, C. S. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.8.3917-3925.1990 · Cited by 177
Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family.
Hanks, S. K. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.2.388 · Cited by 177
Drag&Drop cloning in yeast
Jansen, G., Wu, C., Schade, B., Thomas, D. Y., & Whiteway, M. S. (2004) · Gene · doi:10.1016/j.gene.2004.10.016 · Cited by 176
Characterization of Escherichia coli Strains from Cases of Childhood Diarrhea in Provincial Southwestern Nigeria
Okeke, I. N., Lamikanra, A., Steinrück, H., & Kaper, J. B. (2000) · Journal of Clinical Microbiology · doi:10.1128/jcm.38.1.7-12.2000 · Cited by 176
Subcellular localization and immunological detection of proteins encoded by the vir locus of Bordetella pertussis
Stibitz, S., & Yang, M. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.14.4288-4296.1991 · Cited by 180
Analysis of the COL1A1 and COL1A2 Genes by PCR Amplification and Scanning by Conformation-Sensitive Gel Electrophoresis Identifies Only COL1A1 Mutations in 15 Patients with Osteogenesis Imperfecta Type I: Identification of Common Sequences of Null-Allele Mutations
Körkkö, J., Ala‐Kokko, L., Paepe, A. D., Nuytinck, L., Earley, J. J., & Prockop, D. J. (1998) · The American Journal of Human Genetics · doi:10.1086/301689 · Cited by 176
Genetic Transformation of Banana and Plantain (Musa spp.) via Particle Bombardment
Sági, L., Panis, B., Remy, S., Schoofs, H., Smet, K. D., Swennen, R. L., & Cammue, B. P. A. (1995) · Nature Biotechnology · doi:10.1038/nbt0595-481 · Cited by 176
Human substance P receptor (NK-1): organization of the gene, chromosome localization and functional expression of cDNA clones
Gerard, N. P., Garraway, L. A., Eddy, R. L., Shows, T. B., Iijima, H., Paquet, J. L., & Gerard, C. J. (1991) · Biochemistry · doi:10.1021/bi00108a006 · Cited by 176
Heterogeneous Virulence of EnteroaggregativeEscherichia coliStrains Isolated from Children in Southwest Nigeria
Okeke, I. N., Lamikanra, A., Czeczulin, J. R., Dubovsky, F., Kaper, J. B., & Nataro, J. P. (2000) · The Journal of Infectious Diseases · doi:10.1086/315204 · Cited by 176
Mip protein of Legionella pneumophila exhibits peptidyl‐prolyl‐cis/trans isomerase (PPIase) activity
Fischer, G., Bang, H., Ludwig, B., Mann, K., & Hacker, J. (1992) · Molecular Microbiology · doi:10.1111/j.1365-2958.1992.tb00858.x · Cited by 176
The RcsB–RcsC regulatory system of Salmonella typhi differentially modulates the expression of invasion proteins, flagellin and Vi antigen in response to osmolarity
Arricau, N., Hermant, D., Waxin, H., Ecobichon, C., Duffey, P. S., & Popoff, M. Y. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.00976.x · Cited by 182
A Simple and Rapid Method for Genetic Transformation of Lactic Streptococci by Electroporation
Powell, I. B., Achen, M. G., Hillier, A. J., & Davidson, B. E. (1988) · Applied and Environmental Microbiology · doi:10.1128/aem.54.3.655-660.1988 · Cited by 176
Alteration of a Yeast SH3 Protein Leads to Conditional Viability with Defects in Cytoskeletal and Budding Patterns
Bauer, F. F., Urdaci, M. C., Aigle, M., & Crouzet, M. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.8.5070 · Cited by 176
Physical and genetic map of a Rhizobium meliloti nodulation gene region and nucleotide sequence of nodC
Jacobs, T. W., Egelhoff, T. T., & Long, S. R. (1985) · Journal of Bacteriology · doi:10.1128/jb.162.2.469-476.1985 · Cited by 176
Genetics of digalactoside-binding adhesin from a uropathogenic Escherichia coli strain
Normark, S. J., Lark, D., Hull, R. A., Norgren, M., Båga, M., O'Hanley, P. D., Schoolnik, G. K., & Falkow, S. (1983) · Infection and Immunity · doi:10.1128/iai.41.3.942-949.1983 · Cited by 176
Production of Cytolethal Distending Toxins by Pathogenic Escherichia coli Strains Isolated from Human and Animal Sources: Establishment of the Existence of a New cdt Variant (Type IV)
Tóth, I., Hérault, F., Beutin, L., & Oswald, É. (2003) · Journal of Clinical Microbiology · doi:10.1128/jcm.41.9.4285-4291.2003 · Cited by 175
Identification and cloning of a hemin storage locus involved in the pigmentation phenotype of Yersinia pestis
Perry, R. D., Pendrak, M. L., & Schuetze, P. (1990) · Journal of Bacteriology · doi:10.1128/jb.172.10.5929-5937.1990 · Cited by 175
A Nine-Residue Synthetic Propeptide Enhances Secretion Efficiency of Heterologous Proteins in Lactococcus lactis
Loir, Y. L., Gruss, A., Ehrlich, S. D., & Langella, P. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.7.1895-1903.1998 · Cited by 175
Efficient plasma membrane expression of a functional platelet glycoprotein Ib-IX complex requires the presence of its three subunits.
López, J. A., Leung, B., Reynolds, C. C., Li, C. Q., & Fox, J. E. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)42354-x · Cited by 175
Nucleotide sequence and genetic organization of the Bacillus subtilis comG operon
Albano, M., Breitling, R., & Dubnau, D. A. (1989) · Journal of Bacteriology · doi:10.1128/jb.171.10.5386-5404.1989 · Cited by 175
Construction of full-length cDNA clones of cucumber mosaic virus RNAs 1, 2 and 3: Generation of infectious RNA transcripts
Rizzo, T. M., & Palukaitis, P. (1990) · Molecular and General Genetics MGG · doi:10.1007/bf00633825 · Cited by 175
A yeast replication origin consists of multiple copies of a small conserved sequence
Palzkill, T. G., & Newlon, C. S. (1988) · Cell · doi:10.1016/0092-8674(88)90164-x · Cited by 175
Structure, expression and regulation of a nuclear gene encoding a mitochondrial protein: the yeast L(+)‐lactate cytochrome c oxidoreductase (cytochrome b2).
Guiard, B. (1985) · The EMBO Journal · doi:10.1002/j.1460-2075.1985.tb04076.x · Cited by 175
Identification of multiple HTF‐island associated genes in the human major histocompatibility complex class III region.
Sargent, C. A., Dunham, I., & Campbell, R. D. (1989) · The EMBO Journal · doi:10.1002/j.1460-2075.1989.tb08357.x · Cited by 175
Facilitated diffusion during catalysis by EcoRI endonuclease. Nonspecific interactions in EcoRI catalysis.
Terry, B., Jack, W. E., & Modrich, P. L. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)38848-8 · Cited by 174
Expression and regulation of the antimonite, arsenite, and arsenate resistance operon of Staphylococcus xylosus plasmid pSX267
Rosenstein, R., Peschel, A., Wieland, B., & Götz, F. (1992) · Journal of Bacteriology · doi:10.1128/jb.174.11.3676-3683.1992 · Cited by 174
Polymerase chain reaction identification of Vibrio vulnificus in artificially contaminated oysters
Hill, W. E., Keasler, S. P., Trucksess, M. W., Feng, P., Kaysner, C. A., & Lampel, K. A. (1991) · Applied and Environmental Microbiology · doi:10.1128/aem.57.3.707-711.1991 · Cited by 176
Cloning, nucleotide sequence, and expression in Escherichia coli of the gene for poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis
Saito, T., Suzuki, K., Yamamoto, J., Fukui, T., Miwa, K., Tomita, K., Nakanishi, S., Odani, S., Suzuki, J., & Ishikawa, K. (1989) · Journal of Bacteriology · doi:10.1128/jb.171.1.184-189.1989 · Cited by 174
Diffuse-Adhering Escherichia coli (DAEC) as a Putative Cause of Diarrhea in Mayan Children in Mexico
Gioon, J. A., Jones, T., Millan-Velasco, F., Castro-Munoz, E., Zárate, L. L., Fry, J., Frankel, G. M., Moseley, S. L., Baudry, B., Kaper, J. B., et al. (1991) · The Journal of Infectious Diseases · doi:10.1093/infdis/163.3.507 · Cited by 174
Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae
Clark‐Curtiss, J. E., Jacobs, W. R., Docherty, M., Ritchie, L. R., & Curtiss, R. (1985) · Journal of Bacteriology · doi:10.1128/jb.161.3.1093-1102.1985 · Cited by 174
Amino acid sequence of alpha-amylase from Bacillus amyloliquefaciens deduced from the nucleotide sequence of the cloned gene.
Takkinen, K., Pettersson, R. F., Kalkkinen, N., Palva, I., Söderlund, H., & Kääriäinen, L. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)33151-x · Cited by 174
LcrD, a membrane-bound regulator of the Yersinia pestis low-calcium response
Plano, G. V., Barve, S. S., & Straley, S. C. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.22.7293-7303.1991 · Cited by 174
Secretion and autoproteolytic maturation of subtilisin.
Power, S., Adams, R. M., & Wells, J. A. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.10.3096 · Cited by 174
Common 82-nucleotide sequence unique to brain RNA.
Sutcliffe, J. G., Milner, R. J., Bloom, F. E., & Lerner, R. A. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.16.4942 · Cited by 174
Expression Cloning of Oncogenes by Retroviral Transfer of cDNA Libraries
Whitehead, I. P., Kirk, H., & Kay, R. J. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.2.704 · Cited by 174
MICROBIAL RESISTANCE TO ANTIBIOTICS
Levy, S. (1982) · The Lancet · doi:10.1016/s0140-6736(82)91701-9 · Cited by 173
Characterization of the plasmidic beta-lactamase CMY-2, which is responsible for cephamycin resistance
Bauernfeind, A., Stemplinger, I., Jungwirth, R., & Giamarellou, H. (1996) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.40.1.221 · Cited by 173
Modular organization of the lytic enzymes of Streptococcus pneumoniae and its bacteriophages
Garcı́a, P., García, J., Garcı́a, E., Sánchez-Puelles, J.-M., & López, R. (1990) · Gene · doi:10.1016/0378-1119(90)90116-9 · Cited by 173
Keratin gene expression in mouse epidermis and cultured epidermal cells.
Roop, D. R., Hawley‐Nelson, P., Cheng, C., & Yuspa, S. H. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.3.716 · Cited by 174
Nucleotide sequence of a macronuclear DNA molecule coding for α-tubulin from the ciliateStylonychia lemnae. Special codon usage: TAA is not a translation termination codon
Helftenbem, E. (1985) · Nucleic Acids Research · doi:10.1093/nar/13.2.415 · Cited by 173
Erratum The opacity proteins of Neisseria gonorrhoeae strain MS11 are encoded by a family of 11 complete genes
Bhat, K. S., Gibbs, C. P., Barrera, O., Morrison, S. G., Jähnig, F., Stem, A., Kupsch, E.-M., Meyer, T. F., & Swanson, J. (1992) · Molecular Microbiology · doi:10.1111/j.1365-2958.1992.tb02172.x · Cited by 173
Optimization of Surfactin Production by Bacillus subtilis Isolate BS5
Abdel‐Mawgoud, A. M., Aboulwafa, M., & Hassouna, N. A.-H. (2008) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-008-8155-x · Cited by 172
High-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae: a new vector for high-level expression
Lopes, T. S., Klootwijk, J., Veenstra, A. E., van der Aar, P. C., van Heerikhuizen, H., Raué, H. A., & Planta, R. J. (1989) · Gene · doi:10.1016/0378-1119(89)90202-3 · Cited by 172
Infectivity of the cloned geminivirus genome requires sequences from both DNAs
Stanley, J. (1983) · Nature · doi:10.1038/305643a0 · Cited by 172
Molecular structure and polymorphic map of the human phenylalanine hydroxylase gene
DiLella, A. G., Kwok, S. C. M., Ledley, F. D., Marvit, J., & Woo, S. L. C. (1986) · Biochemistry · doi:10.1021/bi00352a001 · Cited by 172
The human neurokinin A (substance K) receptor. Molecular cloning of the gene, chromosome localization, and isolation of cDNA from tracheal and gastric tissues
Gerard, N. P., Eddy, R. L., Shows, T. B., & Gerard, C. J. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)30526-4 · Cited by 172
Application of DNA-DNA colony hybridization to the detection of catabolic genotypes in environmental samples
Sayler, G. S., Shields, M. S., Tedford, E. T., Breen, A., Hooper, S. W., Sirotkin, K. M., & Davis, J. W. (1985) · Applied and Environmental Microbiology · doi:10.1128/aem.49.5.1295-1303.1985 · Cited by 172
A new method for the detection of alkane-monooxygenase homologous genes (alkB) in soils based on PCR-hybridization
Kloos, K., Munch, J. C., & Schloter, M. (2006) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2006.01.014 · Cited by 171
A Coxiella burnetti repeated DNA element resembling a bacterial insertion sequence
Hoover, T. A., Vodkin, M. H., & Williams, J. C. (1992) · Journal of Bacteriology · doi:10.1128/jb.174.17.5540-5548.1992 · Cited by 171
Intra- and intramolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase
Dale, E. C., & Ow, D. W. (1990) · Gene · doi:10.1016/0378-1119(90)90165-n · Cited by 171
Physiological properties of a Pseudomonas strain which grows with p-xylene in a two-phase (organic-aqueous) medium
Cruden, D. L., Wolfram, J. H., Rogers, R. D., & Gibson, D. T. (1992) · Applied and Environmental Microbiology · doi:10.1128/aem.58.9.2723-2729.1992 · Cited by 171
Uropathogenic, Escherichia coli can express serologically identical pili of different receptor binding specificities
Lund, B., Marklund, B. ‐., Strömberg, N., Lindberg, F. P., Karlsson, K., & Normark, S. J. (1988) · Molecular Microbiology · doi:10.1111/j.1365-2958.1988.tb00027.x · Cited by 171
Glucoamylases G1 and G2 from Aspergillus niger are synthesized from two different but closely related mRNAs.
Boel, E., Hjort, I., Svensson, B., Norris, F., Norris, K. E., & Fiil, N. P. (1984) · The EMBO Journal · doi:10.1002/j.1460-2075.1984.tb01935.x · Cited by 171
Suppression of the btuB451 mutation by mutations in the tonB gene suggests a direct interaction between TonB and TonB-dependent receptor proteins in the outer membrane of Escherichia coli
Heller, K. J., Kadner, R. J., & Günther, K. (1988) · Gene · doi:10.1016/0378-1119(88)90488-x · Cited by 171
Chloroplast ATP synthase of spinach contains nine nonidentical subunit species, six of which are encoded by plastid chromosomes in two operons in a phylogenetically conserved arrangement
Hennig, J., & Herrmann, R. G. (1986) · Molecular and General Genetics MGG · doi:10.1007/bf00330392 · Cited by 171
Application of monoliths for plasmid DNA purification
Urthaler, J., Schlegl, R., Podgornik, A., Štrancar, A., Jungbauer, A., & Necina, R. (2004) · Journal of Chromatography A · doi:10.1016/j.chroma.2004.12.007 · Cited by 170
Transcriptional regulation of Bacillus subtilis glucose starvation-inducible genes: control of gsiA by the ComP-ComA signal transduction system
Mueller, J. P., Bukusoglu, G., & Sonenshein, A. L. (1992) · Journal of Bacteriology · doi:10.1128/jb.174.13.4361-4373.1992 · Cited by 170
Molecular evolution of lytic enzymes of Streptococcus pneumoniae and its bacteriophages.
Garcı́a, E., García, J. L., Garcı́a, P., Arrarás, A., Sánchez-Puelles, J.-M., & López, R. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.3.914 · Cited by 170
Amino acid sequence of rat kidney gamma-glutamylcysteine synthetase.
Yan, N., & Meister, A. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)40057-4 · Cited by 170
Sandwich hybridization as a convenient method for the detection of nucleic acids in crude samples
Ranki, M., Palva, A., Virtanen, M., Laaksonen, M., & Söderlund, H. (1983) · Gene · doi:10.1016/0378-1119(83)90149-x · Cited by 170
Structure of the gene encoding the exoglucanase of Cellulomonas fimi
O’Neill, G. P., Goh, S. H., Warren, R. A. J., Kilburn, D. G., & Miller, R. C. (1986) · Gene · doi:10.1016/0378-1119(86)90197-6 · Cited by 170
Molecular cloning and characterization of the cDNA coding for C4b-binding protein, a regulatory protein of the classical pathway of the human complement system
Chung, L.-P., Bentley, D. R., & Reid, K. B. M. (1985) · Biochemical Journal · doi:10.1042/bj2300133 · Cited by 170
Characterization of the Expressed Gene and Several Processed Pseudogenes for the Mouse Ribosomal Protein L30 Gene Family
Wiedemann, L. M., & Perry, R. P. (1984) · Molecular and Cellular Biology · doi:10.1128/mcb.4.11.2518 · Cited by 170
Correction of xeroderma pigmentosum complementation group D mutant cell phenotypes by chromosome and gene transfer: involvement of the human ERCC2 DNA repair gene.
Flejter, W. L., McDaniel, L., Johns, D., Friedberg, E. C., & Schultz, R. A. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.1.261 · Cited by 170
Role of Catalase in Campylobacter jejuni Intracellular Survival
Day, W. A., Sajecki, J. L., Pitts, T. M., & Joens, L. A. (2000) · Infection and Immunity · doi:10.1128/iai.68.11.6337-6345.2000 · Cited by 169
Mutations in SPT16/CDC68 Suppress cis- and trans-Acting Mutations That Affect Promoter Function in Saccharomyces cerevisiae
Malone, E. A., Clark, C. D., Chiang, A., & Winston, F. (1991) · Molecular and Cellular Biology · doi:10.1128/mcb.11.11.5710 · Cited by 169
Yersinia pestis pH 6 antigen: genetic, biochemical, and virulence characterization of a protein involved in the pathogenesis of bubonic plague
Lindler, L. E., Klempner, M. S., & Straley, S. C. (1990) · Infection and Immunity · doi:10.1128/iai.58.8.2569-2577.1990 · Cited by 169
Nucleotide sequence of the thermostable direct hemolysin gene of Vibrio parahaemolyticus
Nishibuchi, M., & Kaper, J. B. (1985) · Journal of Bacteriology · doi:10.1128/jb.162.2.558-564.1985 · Cited by 169
Complete nucleotide sequence of phoE, the structural gene for the phosphate limitation inducible outer membrane pore protein of Escherichia coli K12
Overbeeke, N., Bergmans, H. E. N., van Mansfeld, F., & Lugtenberg, B. J. J. (1983) · Journal of Molecular Biology · doi:10.1016/0022-2836(83)90110-9 · Cited by 169
Identification of Basal and Cyclic AMP Regulatory Elements in the Promoter of the Phosphoenolpyruvate Carboxykinase Gene
Quinn, P. G., Wong, T. W., Magnuson, M. A., Shabb, J. B., & Granner, D. K. (1988) · Molecular and Cellular Biology · doi:10.1128/mcb.8.8.3467 · Cited by 169
Versatile expression vectors for high-level synthesis of cloned gene products in Escherichia coli
Crowl, R. M., Seamans, C., Lomedico, P. T., & McAndrew, S. J. (1985) · Gene · doi:10.1016/0378-1119(85)90200-8 · Cited by 169
The extracellular matrix protects Bacillus subtilis colonies from Pseudomonas invasion and modulates plant co-colonization
Molina‐Santiago, C., Pearson, J. R., Navarro, Y., Berlanga‐Clavero, M. V., Caraballo‐Rodríguez, A. M., Petras, D., García‐Martín, M. L., Lamon, G., Haberstein, B., Cazorla, F. M., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-09944-x · Cited by 182
Development of a rapid and efficient restriction endonuclease analysis typing system for Clostridium difficile and correlation with other typing systems
Clabots, C., Johnson, S., Bettin, K. M., Mathie, P., Mulligan, M. E., Schaberg, D. R., Peterson, L. R., & Gerding, D. N. (1993) · Journal of Clinical Microbiology · doi:10.1128/jcm.31.7.1870-1875.1993 · Cited by 168
Transformation of Helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker
Wang, Y., Roos, K. P., & Taylor, D. E. (1993) · Journal of General Microbiology · doi:10.1099/00221287-139-10-2485 · Cited by 168
Helicobacter pylori nickel‐transport gene nixA: synthesis of catalytically active urease in Escherichia coli independent of growth conditions
Mobley, H. L. T., Garner, R. M., & Bauerfeind, P. (1995) · Molecular Microbiology · doi:10.1111/j.1365-2958.1995.tb02395.x · Cited by 168
Decay‐accelerating factor CD55 is identified as the receptor for echovirus 7 using CELICS, a rapid immuno‐focal cloning method.
Ward, T., Pipkin, P. A., Clarkson, N. A., Stone, D. M., Minor, P. D., & Almond, J. W. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06836.x · Cited by 168
Intratumoral injection of an adenovirus expressing interleukin 2 induces regression and immunity in a murine breast cancer model.
Addison, C., Braciak, T. A., Ralston, R., Muller, W. J., Gauldie, J., & Graham, F. L. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.18.8522 · Cited by 168
Cloning of the chromosomal determinants encoding hemolysin production and mannose-resistant hemagglutination in Escherichia coli
Berger, H., Hacker, J. H., Juárez, A., Hughes, C., & Goebel, W. (1982) · Journal of Bacteriology · doi:10.1128/jb.152.3.1241-1247.1982 · Cited by 168
Nucleotide sequence and expression of the pneumococcal autolysin gene from its own promoter in Escherichia coli
Garcı́a, P., García, J. L., Garcı́a, E., & López, R. (1986) · Gene · doi:10.1016/0378-1119(86)90215-5 · Cited by 168
Identification of genes and gene products involved in the nodulation of peas by Rhizobium leguminosarum
Downie, J. A., Knight, C. D., Johnston, A. W. B., & Rossen, L. (1985) · Molecular and General Genetics MGG · doi:10.1007/bf00383003 · Cited by 168
A yeast operator overlaps an upstream activation site
Kronstad, J. W., Holly, J., & MacKay, V. L. (1987) · Cell · doi:10.1016/0092-8674(87)90491-0 · Cited by 168
Immunochemical characterization of and isolation of the gene for a Borrelia burgdorferi immunodominant 60-kilodalton antigen common to a wide range of bacteria
Hansen, K., Bangsborg, J., Fjordvang, H., Pedersen, N. S., & Hindersson, P. (1988) · Infection and Immunity · doi:10.1128/iai.56.8.2047-2053.1988 · Cited by 168
Mutational analysis of the virulence region of an Agrobacterium tumefaciens Ti plasmid
Klee, H. J., White, F. F., Iyer, V. N., Gordon, M. P., & Nester, E. W. (1983) · Journal of Bacteriology · doi:10.1128/jb.153.2.878-883.1983 · Cited by 168
Carbapenem-resistant Escherichia coli and Klebsiella pneumoniae isolates from Turkey with OXA-48-like carbapenemases and outer membrane protein loss
Gülmez, D., Woodford, N., Palepou, M.-F. I., Mushtaq, S., Metan, G., Yakupoğulları, Y., Kocagöz, S., Uzun, Ö., Hasçelik, G., & Livermore, D. M. (2008) · International Journal of Antimicrobial Agents · doi:10.1016/j.ijantimicag.2008.01.017 · Cited by 167
Overexpression and purification of ferric enterobactin esterase from Escherichia coli. Demonstration of enzymatic hydrolysis of enterobactin and its iron complex.
Brickman, T. J., & McIntosh, M. A. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)49846-3 · Cited by 167
Erv1p from Saccharomyces cerevisiae is a FAD‐linked sulfhydryl oxidase
Lee, J.-E., Hofhaus, G., & Lisowsky, T. (2000) · FEBS Letters · doi:10.1016/s0014-5793(00)01767-1 · Cited by 167
Cloning and molecular characterization of the murine herpesvirus 68 genome
Efstathiou, S., Ho, Y.-S., & Minson, A. C. (1990) · Journal of General Virology · doi:10.1099/0022-1317-71-6-1355 · Cited by 167
A maturation protein is essential for production of active forms of Lactococcus lactis SK11 serine proteinase located in or secreted from the cell envelope
Vos, P., van Asseldonk, M., van Jeveren, F., Siezen, R. J., Simons, G., & de Vos, W. M. (1989) · Journal of Bacteriology · doi:10.1128/jb.171.5.2795-2802.1989 · Cited by 168
Exonucleolytic proofreading enhances the fidelity of DNA synthesis by chick embryo DNA polymerase-gamma.
Kunkel, T. A., & Soni, A. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)68947-1 · Cited by 167
Nucleotide Sequence of Potato Leafroll Luteovirus RNA
Mayo, M. A., Robinson, D. J., Jolly, C. A., & Hyman, L. (1989) · Journal of General Virology · doi:10.1099/0022-1317-70-5-1037 · Cited by 167
Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.
Joyce, C. M., & Grindley, N. D. F. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.7.1830 · Cited by 167
Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces
Bibb, M. J., Bibb, M. J., Ward, J. M., & Cohen, S. N. (1985) · Molecular and General Genetics MGG · doi:10.1007/bf00327505 · Cited by 167
A DNA‐based method for identification of krill species and its application to analysing the diet of marine vertebrate predators
Jarman, S., Gales, N. J., Tierney, M., Gill, P. C., & Elliott, N. G. (2002) · Molecular Ecology · doi:10.1046/j.1365-294x.2002.01641.x · Cited by 166
Cloning of novel enterotoxin genes from Bacillus cereus and Bacillus thuringiensis
Asano, S., Nukumizu, Y., Bando, H., Iizuka, T., & Yamamoto, T. (1997) · Applied and Environmental Microbiology · doi:10.1128/aem.63.3.1054-1057.1997 · Cited by 166
The Smooth Muscle α-Actin Gene Promoter Is Differentially Regulated in Smooth Muscle versus Non-smooth Muscle Cells
Shimizu, R. T., Blank, R. S., Jervis, R., Lawrenz-Smith, S. C., & Owens, G. K. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.13.7631 · Cited by 166
Linker-insertion nonsense and restriction-site deletion mutations of the gB glycoprotein gene of herpes simplex virus type 1
Cai, W. Z., Person, S., Warner, S. C., Zhou, J. H., & DeLuca, N. A. (1987) · Journal of Virology · doi:10.1128/jvi.61.3.714-721.1987 · Cited by 166
Both positive and negative regulatory elements mediate expression of a photoregulated CAB gene from Nicotiana plumbaginifolia.
Castresana, C., Garcı́a, I., Alonso, E., Malik, V. S., & Cashmore, A. R. (1988) · The EMBO Journal · doi:10.1002/j.1460-2075.1988.tb03030.x · Cited by 166
Heterogeneity of Escherichia Coli Phages Encoding Vero Cytotoxins: Comparison of Cloned Sequences Determining VT1 and VT2 and Development of Specific Gene Probes
Willshaw, G. A., Smith, H. R., Scotland, S. M., Field, A. M., & Rowe, B. (1987) · Microbiology · doi:10.1099/00221287-133-5-1309 · Cited by 166
Nucleotide sequences of cDNAs encoding precursors of human insulin‐like growth factor II (IGF‐II) and an IGF‐II variant
Jansen, M., van Schaik, P. M. A., van Tol, H., den Brande, J. L. V., & Sussenbach, J. S. (1985) · FEBS Letters · doi:10.1016/0014-5793(85)80527-5 · Cited by 166
The primary structure of theSaccharomyces cerevisiaegene for 3-phosphogrycerate kinase
Hitzeman, R. A., Hagie, F. E., Hayflick, J. S., Chen, C. Y., Seeburg, P. H., & Derynck, R. (1982) · Nucleic Acids Research · doi:10.1093/nar/10.23.7791 · Cited by 166

Authors: Birnboim, H. C., & Doly, J.
Affiliations: Laboratoire de Génétique Moléculaire, Institut de Recherche en Biologie Moleculaire, F-75221 Paris Cedex 05, France
ORCID: Birnboim, H.C.
Venue: Nucleic Acids Research
Publisher: Oxford University Press (OUP)
Published: 1979-11-24
Volume: 7
Issue: 6
Pages: 1513-1523
ISSN: 0305-1048 (ISSN-L)
1362-4962
DOI: 10.1093/nar/7.6.1513
PMC: PMC342324
OpenAlex: W2019410656
Cited by: 14,686
References: 20
Citations by year: 2026: 27 · 2025: 39 · 2024: 51 · 2023: 59 · 2022: 76 · 2021: 100 · 2020: 85 · 2019: 100 · 2018: 97 · 2017: 101 · 2016: 123 · 2015: 99 · 2014: 147 · 2013: 195 · 2012: 175
FWCI: 90.00
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: Nucleic Acids Research (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords: plasmid DNA extraction, alkaline extraction, recombinant plasmid, bacterial clones, DNA purification
MeSH terms: Animals, Chromosomes, Bacterial, Cloning, Molecular, DNA Restriction Enzymes, DNA, Circular, DNA, Recombinant, Escherichia coli, Methods, Molecular Weight, Plasmids, Transformation, Bacterial, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks