The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Open accessAbstract
A procedure for extracting plasmid DNA from bacterial cells 1s described. The method 1s simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circular DNA remains double-stranded. Adequate pH control is accomplished without using a pH meter. Upon neutralization, chromosomal DNA renatures to form an insoluble clot, leaving plasmid DNA in the supernatant. Large and small plasmid DNAs have been extracted by this method.
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“With the multiple-sequencing results, some random errors can be eliminated on the basis of the principle of plurality voting to improve the sequencing accuracy (Fig. 4, C and D) (51, 52).”
From Electrochemical DNA synthesis and sequencing on a single electrode with scalability for integrated data storage (2021) · cites this paper as [52]
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Cites
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.
Bolívar, F. G., Rl, R., Pj, G., Betlach, M. C., Hl, H., Boyer, H. W., Crosa, J. H., & Falkow, S. (1992) · PubMed · Cited by 4,672
A complementation analysis of the restriction and modification of DNA in Escherichia coli
Boyer, H. W., & Roulland-Dussoix, D. (1969) · Journal of Molecular Biology · doi:10.1016/0022-2836(69)90288-5 · Cited by 3,768
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Bolivar, F., Rodriguez, R. L., Greene, P. J., Betlach, M. C., Heyneker, H. L., Boyer, H. W., Crosa, J. H., & Falkow, S. (1977) · PubMed · Cited by 2,750
Clewell, D. B., & Helinski, D. R. (1969) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.62.4.1159 · Cited by 1,801
Nature of Col E 1 Plasmid Replication in Escherichia coli in the Presence of Chloramphenicol
Clewell, D. B. (1972) · Journal of Bacteriology · doi:10.1128/jb.110.2.667-676.1972 · Cited by 1,166
A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria
Eckhardt, T. (1978) · Plasmid · doi:10.1016/0147-619x(78)90016-1 · Cited by 945
THE PREPARATION AND CHARACTERIZATION OF RIBONUCLEIC ACIDS FROM YEAST
Crestfield, A. M., Smith, K. C., & Allen, F. W. (1955) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52295-5 · Cited by 440
Isolation of covalently closed circular DNA of high molecular weight from bacteria
Currier, T. C., & Nester, E. W. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90338-9 · Cited by 408
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli
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Collins, J. F., & Höhn, B. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.9.4242 · Cited by 360
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Feinberg, A. P., & Vogelstein, B. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90418-9 · Cited by 24,512
Yanisch-Perron, C., Vieira, J., & Messing, J. W. (1985) · Gene · doi:10.1016/0378-1119(85)90120-9 · Cited by 14,909
16S ribosomal DNA amplification for phylogenetic study
Weisburg, W. G., Barns, S. M., Pelletier, D. A., & Lane, D. J. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.2.697-703.1991 · Cited by 11,970
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
Felgner, P. L., Gadek, T. R., Holm, M., Roman, R., Chan, H. W., Wenz, M., Northrop, J. P., RINGOLD, G. M., & Danielsen, M. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.21.7413 · Cited by 4,954
Frohman, M. A., Dush, M. K., & Martin, G. R. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.23.8998 · Cited by 4,554
Metadata
- Authors
- Birnboim, H. C., & Doly, J.
- Affiliations
- Laboratoire de Génétique Moléculaire, Institut de Recherche en Biologie Moleculaire, F-75221 Paris Cedex 05, France
- ORCID
- Birnboim, H.C.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1979-11-24
- Volume
- 7
- Issue
- 6
- Pages
- 1513-1523
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC342324
- OpenAlex
- W2019410656
- Cited by
- 14,686
- References
- 20
- Citations by year
- 2026: 27 · 2025: 39 · 2024: 51 · 2023: 59 · 2022: 76 · 2021: 100 · 2020: 85 · 2019: 100 · 2018: 97 · 2017: 101 · 2016: 123 · 2015: 99 · 2014: 147 · 2013: 195 · 2012: 175
- FWCI
- 90.00
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Nucleic Acids Research (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- plasmid DNA extraction, alkaline extraction, recombinant plasmid, bacterial clones, DNA purification
- MeSH terms
- Animals, Chromosomes, Bacterial, Cloning, Molecular, DNA Restriction Enzymes, DNA, Circular, DNA, Recombinant, Escherichia coli, Methods, Molecular Weight, Plasmids, Transformation, Bacterial, Mice
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