Cited by 14,686Molecular Biology Techniques and ApplicationsBacterial Genetics and BiotechnologyDNA and Nucleic Acid Chemistry

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A rapid alkaline extraction procedure for screening recombinant plasmid DNA

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Birnboim, H.C., Doly, J.·1979·Nucleic Acids Research

DOI 10.1093/nar/7.6.1513PMC PMC342324

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Abstract

A procedure for extracting plasmid DNA from bacterial cells 1s described. The method 1s simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circular DNA remains double-stranded. Adequate pH control is accomplished without using a pH meter. Upon neutralization, chromosomal DNA renatures to form an insoluble clot, leaving plasmid DNA in the supernatant. Large and small plasmid DNAs have been extracted by this method.

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Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.
Bolívar, F. G., Rl, R., Pj, G., Betlach, M. C., Hl, H., Boyer, H. W., Crosa, J. H., & Falkow, S. (1992) · PubMed · Cited by 4,672
A complementation analysis of the restriction and modification of DNA in Escherichia coli
Boyer, H. W., & Roulland-Dussoix, D. (1969) · Journal of Molecular Biology · doi:10.1016/0022-2836(69)90288-5 · Cited by 3,768
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Bolivar, F., Rodriguez, R. L., Greene, P. J., Betlach, M. C., Heyneker, H. L., Boyer, H. W., Crosa, J. H., & Falkow, S. (1977) · PubMed · Cited by 2,750
SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN Escherichia coli : PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM
Clewell, D. B., & Helinski, D. R. (1969) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.62.4.1159 · Cited by 1,830
Nature of Col E 1 Plasmid Replication in Escherichia coli in the Presence of Chloramphenicol
Clewell, D. B. (1972) · Journal of Bacteriology · doi:10.1128/jb.110.2.667-676.1972 · Cited by 1,193
A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria
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THE PREPARATION AND CHARACTERIZATION OF RIBONUCLEIC ACIDS FROM YEAST
Crestfield, A. M., Smith, K. C., & Allen, F. W. (1955) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52295-5 · Cited by 440
Isolation of covalently closed circular DNA of high molecular weight from bacteria
Currier, T. C., & Nester, E. W. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90338-9 · Cited by 408
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli
Sharp, P. A., Hsu, M., Ohtsubo, E., & Davidson, N. (1972) · Journal of Molecular Biology · doi:10.1016/0022-2836(72)90363-4 · Cited by 380
Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads.
Collins, J. F., & Höhn, B. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.9.4242 · Cited by 360

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[17] A rapid alkaline extraction method for the isolation of plasmid DNA
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Arthur, M., Molinas, C., Depardieu, F., & Courvalin, P. M. (1993) · Journal of Bacteriology · doi:10.1128/jb.175.1.117-127.1993 · Cited by 600
Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli
Misawa, N., Nakagawa, M., Kobayashi, K., Yamano, S., Izawa, Y., Nakamura, K., & Harashima, K. (1990) · Journal of Bacteriology · doi:10.1128/jb.172.12.6704-6712.1990 · Cited by 596
Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype
Wells, J. G., DAVIS, B. R., Wachsmuth, I. K., Riley, L. W., Remis, R. S., Sokolow, R., & Morris, G. K. (1983) · Journal of Clinical Microbiology · doi:10.1128/jcm.18.3.512-520.1983 · Cited by 580
Pichia pastoris as a Host System for Transformations
Cregg, J. M., Barringer, K., Hessler, A. Y., & Madden, K. R. (1985) · Molecular and Cellular Biology · doi:10.1128/mcb.5.12.3376-3385.1985 · Cited by 575
Resistance-Nodulation-Cell Division-Type Efflux Pump Involved in Aminoglycoside Resistance in Acinetobacter baumannii Strain BM4454
Magnet, S., Courvalin, P. M., & Lambert, T. (2001) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.45.12.3375-3380.2001 · Cited by 574
Genetic analysis of crown gall: Fine structure map of the T-DNA by site-directed mutagenesis
Garfinkel, D. J. (1981) · Cell · doi:10.1016/0092-8674(81)90368-8 · Cited by 574
Complete sequence of the staphylococcal gene encoding protein A. A gene evolved through multiple duplications.
Uhlén, M., Guss, B., Nilsson, B., Gatenbeck, S., Philipson, L., & Lindberg, M. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)43463-6 · Cited by 555
A Review on Macroscale and Microscale Cell Lysis Methods
Islam, M. S., Aryasomayajula, A., & Selvaganapathy, P. R. (2017) · Micromachines · doi:10.3390/mi8030083 · Cited by 555
Wide host range cloning vectors: A cosmid clone bank of an Agrobacterium Ti plasmid
Knauf, V. C., & Nester, E. W. (1982) · Plasmid · doi:10.1016/0147-619x(82)90040-3 · Cited by 555
Pyelonephritogenic Escherichia coli and killing of cultured human renal proximal tubular epithelial cells: role of hemolysin in some strains
Mobley, H. L. T., Green, D. M., Trifillis, A. L., Johnson, D. E., Chippendale, G. R., Lockatell, C. V., Jones, B. D., & Warren, J. W. (1990) · Infection and Immunity · doi:10.1128/iai.58.5.1281-1289.1990 · Cited by 550
Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity.
Johnson, D. I., & Pringle, J. R. (1990) · The Journal of Cell Biology · doi:10.1083/jcb.111.1.143 · Cited by 543
Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen
Lanford, R. E., & Butel, J. S. (1984) · Cell · doi:10.1016/0092-8674(84)90415-x · Cited by 542
Primary structure of an extracellular matrix proteoglycan core protein deduced from cloned cDNA.
Krusius, T., & Ruoslahti, E. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.20.7683 · Cited by 529
Identification of a novel transposon-associated phosphoethanolamine transferase gene, mcr-5, conferring colistin resistance in d-tartrate fermenting Salmonella enterica subsp. enterica serovar Paratyphi B
Borowiak, M., Fischer, J., Hammerl, J. A., Hendriksen, R. S., Szabó, I., & Malorny, B. (2017) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkx327 · Cited by 527
Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes
White, F. F., Taylor, B. H., Huffman, G. A., Gordon, M. P., & Nester, E. W. (1985) · Journal of Bacteriology · doi:10.1128/jb.164.1.33-44.1985 · Cited by 524
Human leukocyte interferon produced by E. coli is biologically active
Goeddel, D. V., Yelverton, E., Ullrich, A., Heyneker, H. L., Miozzari, G., Holmes, W., Seeburg, P. H., Dull, T. J., May, L., STEBBING, N., et al. (1980) · Nature · doi:10.1038/287411a0 · Cited by 520

Authors: Birnboim, H. C., & Doly, J.
Affiliations: Laboratoire de Génétique Moléculaire, Institut de Recherche en Biologie Moleculaire, F-75221 Paris Cedex 05, France
ORCID: Birnboim, H.C.
Venue: Nucleic Acids Research
Publisher: Oxford University Press (OUP)
Published: 1979-11-24
Volume: 7
Issue: 6
Pages: 1513-1523
ISSN: 0305-1048 (ISSN-L)
1362-4962
DOI: 10.1093/nar/7.6.1513
PMC: PMC342324
OpenAlex: W2019410656
Cited by: 14,686
References: 20
Citations by year: 2026: 27 · 2025: 39 · 2024: 51 · 2023: 59 · 2022: 76 · 2021: 100 · 2020: 85 · 2019: 100 · 2018: 97 · 2017: 101 · 2016: 123 · 2015: 99 · 2014: 147 · 2013: 195 · 2012: 175
FWCI: 90.00
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: Nucleic Acids Research (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords: plasmid DNA extraction, alkaline extraction, recombinant plasmid, bacterial clones, DNA purification
MeSH terms: Animals, Chromosomes, Bacterial, Cloning, Molecular, DNA Restriction Enzymes, DNA, Circular, DNA, Recombinant, Escherichia coli, Methods, Molecular Weight, Plasmids, Transformation, Bacterial, Mice

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A rapid alkaline extraction procedure for screening recombinant plasmid DNA

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