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The detection of DNA-binding proteins by protein blotting
Open accessAbstract
A method, called "protein blotting," for the detection of DNA-binding proteins is described. Proteins are separated on an SDA-polyacrylamide gel. The gel is sandwiched between 2 nitrocellulose filters and the proteins allowed to diffuse out of the gel and onto the filters. The proteins are tightly bound to each filter, producing a replica of the original gel pattern. The replica is used to detect DNA-binding proteins, RNA-binding proteins or histone-binding proteins by incubation of the filter with [32P]DNA, [125I]RNA, or [125I] histone. Evidence is also presented that specific protein-DNA interactions may be detected by this technique; under appropriate conditions, the lac repressor binds only to DNA containing the lac operator. Strategies for the detection of specific protein-DNA interactions are discussed.
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Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Self-citation
Cited by
Mitochondrial DNA sequences of primates: tempo and mode of evolution
Brown, W. M., Prager, E. M., Wang, A., & Wilson, A. C. (1982) · Journal of Molecular Evolution · doi:10.1007/bf01734101 · Cited by 1,554
Protein blotting: Principles and applications
Gershoni, J. M., & Palade, G. E. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90128-8 · Cited by 853
DNA, RNA, and Protein Extraction: The Past and The Present
Tan, S. C., & Yiap, B. C. (2009) · BioMed Research International · doi:10.1155/2009/574398 · Cited by 771
Three myosin heavy-chain isozymes appear sequentially in rat muscle development
Whalen, R. G., Sell, S. M., Butler-Browne, G. S., Schwartz, K., Bouveret, P., & Pinset-Härström, I. (1981) · Nature · doi:10.1038/292805a0 · Cited by 578
NGL Viewer: a web application for molecular visualization
Rose, A. S., & Hildebrand, P. W. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv402 · Cited by 462
Metadata
- Authors
- Bowen, B., Steinberg, J., Laemmli, U. K., & Weintraub, H.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1980
- Volume
- 8
- Issue
- 1
- Pages
- 1-20
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2008148453
- Cited by
- 726
- References
- 1
- FWCI
- 52.25
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Bowen, B., Steinberg, J., Laemmli, U. K., & Weintraub, H. (1980). The detection of DNA-binding proteins by protein blotting. Nucleic Acids Research. https://doi.org/10.1093/nar/8.1.1
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
