The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Rapid and efficient cosmid cloning
Open accessAbstract
We present a procedure for cosmid cloning that allows rapid and efficient cloning of individual DNA fragments of between 32kb and 45kb. By appropriate treatment of the cloning vector, pJb8, we make left-hand and right-hand vector ends that are incapable of self-ligation but which accept dephosporylated insert DNA fragments. The inserted fragments are generated by partial digestion with MboI or Sau3A and are dephosphorylated to prevent ligation and insertion of non-contiguous fragments. The method eliminates the need to size the insert DNA fragments and prevents formation of clones containing short or multiple inserts. 1 microgram of target Drosophila DNA gives about 5 x 10(5) clones, with an average insert size of 38kb. We also describe a rapid and efficient method for preparing plasmid and cosmid DNA.
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Citations
Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
[17] A rapid alkaline extraction method for the isolation of plasmid DNA
Birnboim, H. C. (1983) · Methods in Enzymology · doi:10.1016/0076-6879(83)00059-2 · Cited by 964
Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factor
Scott, J., Selby, M., Urdea, M., Quiroga, M., Bell, G. I., & Rutter, W. J. (1983) · Nature · doi:10.1038/302538a0 · Cited by 466
Ochsner, U. A., Fiechter, A., & Reiser, J. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)32089-6 · Cited by 332
Weiss, E. H., Golden, L., Fahrner, K., Mellor, A. L., Devlin, J. J., Bullman, H., Tiddens, H., Bud, H., & Flavell, R. A. (1984) · Nature · doi:10.1038/310650a0 · Cited by 316
Bouillaud, F., Ricquier, D., Thibault, J., & Weissenbach, J. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.2.445 · Cited by 244
Metadata
- Authors
- Ish-Horowicz, D., & Burke, J. F.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1981
- Volume
- 9
- Issue
- 13
- Pages
- 2989-2898
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2049635421
- Cited by
- 1,798
- References
- 1
- FWCI
- 51.28
- Type
- journal article
- Language
- en
- Open access
- green
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- Ish-Horowicz, D., & Burke, J. F. (1981). Rapid and efficient cosmid cloning. Nucleic Acids Research. https://doi.org/10.1093/nar/9.13.2989
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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