Molecular approach to thermogenesis in brown adipose tissue: cDNA cloning of the mitochondrial uncoupling protein.

Bouillaud, F; Ricquier, D; Thibault, J; Weissenbach, J

doi:10.1073/pnas.82.2.445

Cited by 244Adipose Tissue and MetabolismMitochondrial Function and PathologyPeroxisome Proliferator-Activated Receptors

Molecular approach to thermogenesis in brown adipose tissue: cDNA cloning of the mitochondrial uncoupling protein.

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Bouillaud, F, Ricquier, D, Thibault, J, Weissenbach, J·1985·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.82.2.445PMC PMC397055

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Abstract

The uncoupling protein (UCP) of mammalian brown fat is a specialized and unique component responsible for energy dissipation as heat. Translation and immunoprecipitation from sucrose-fractionated mRNA indicated that the mRNA of UCP sedimented at 14-16 S. A recombinant cDNA library prepared from mRNA of thermoactive brown fat enriched for UCP mRNA has been constructed and cloned in Escherichia coli. Recombinant plasmids were screened by differential colony hybridization to a cDNA probe complementary to poly(A)+ RNA isolated from thermogenic or from weakly thermogenic brown fat. Several differentially hybridizing plasmids were shown to contain UCP cDNA sequences by their ability to select a mRNA coding for an in vitro translation product that was immunoprecipitable with antibodies against UCP. Blot hybridization of brown fat mRNA to a 32P-labeled UCP cDNA probe revealed two major species of mRNA (15S and 18S). As compared to non-thermogenic tissue, a strikingly increased hybridization to the probe was observed with brown fat mRNA from thermoactive tissue. Moreover, hybridization was observed with RNA of brown adipose tissue from rat, hamster, or mouse but not with RNA from rat or mouse liver.

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UCP1 hinders the establishment of, or dissipates, a proton gradient of sufficient magnitude to sustain the synthetic activity of the mitochondrial ATPase, thus driving continuous accelerated electron transport.
“UCP1 hinders the establishment of, or dissipates, a proton gradient of sufficient magnitude to sustain the synthetic activity of the mitochondrial ATPase (150, 153-155), thus driving continuous accelerated electron transport.”
From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [153]
In brown adipose tissue, electron transport is greatly accelerated due to tissue-specific expression of the mitochondrial UCP1.
“In this tissue, electron transport is greatly accelerated due to tissue-specific expression of the mitochondrial UCP1.”
From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [153]

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Authors: Bouillaud, F., Ricquier, D., Thibault, J., & Weissenbach, J.
Affiliations: Centre National de la Recherche Scientifique (FR)
ORCID: Bouillaud, F
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 1985-01
Volume: 82
Issue: 2
Pages: 445-448
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.82.2.445
PMC: PMC397055
OpenAlex: W2088076536
Cited by: 244
References: 27
Citations by year: 2026: 4 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 8 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 6 · 2015: 4 · 2014: 3 · 2013: 6 · 2012: 6
FWCI: 5.45
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Centre National de la Recherche Scientifique
Topics: Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
Keywords: uncoupling proteins, brown adipose tissue, thermogenesis, cDNA cloning, mitochondrial uncoupling, energy dissipation, tissue-specific gene expression, rats, hamster, mice
MeSH terms: Uncoupling Protein 1, Animals, Body Temperature Regulation, Adipose Tissue, Brown, Carrier Proteins, Centrifugation, Density Gradient, Cloning, Molecular, DNA, Electrophoresis, Polyacrylamide Gel, Ion Channels, Male, Membrane Proteins, Nucleic Acid Hybridization, Plasmids, Rats, Inbred Strains, RNA, Messenger, Mitochondrial Proteins, Rats

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Molecular approach to thermogenesis in brown adipose tissue: cDNA cloning of the mitochondrial uncoupling protein.

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