The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
In vitro selection, characterization, and application of deoxyribozymes that cleave RNA
Open accessAbstract
Over the last decade, many catalytically active DNA molecules (deoxyribozymes; DNA enzymes) have been identified by in vitro selection from random-sequence DNA pools. This article focuses on deoxyribozymes that cleave RNA substrates. The first DNA enzyme was reported in 1994 and cleaves an RNA linkage. Since that time, many other RNA-cleaving deoxyribozymes have been identified. Most but not all of these deoxyribozymes require a divalent metal ion cofactor such as Mg2+ to catalyze attack by a specific RNA 2'-hydroxyl group on the adjacent phosphodiester linkage, forming a 2',3'-cyclic phosphate and a 5'-hydroxyl group. Several deoxyribozymes that cleave RNA have utility for in vitro RNA biochemistry. Some DNA enzymes have been applied in vivo to degrade mRNAs, and others have been engineered into sensors. The practical impact of RNA-cleaving deoxyribozymes should continue to increase as additional applications are developed.
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Cites
Gilbert, W. (1986) · Nature · doi:10.1038/319618a0 · Cited by 2,721
The chemical repertoire of natural ribozymes
Doudna, J. A., & Cech, T. R. (2002) · Nature · doi:10.1038/418222a · Cited by 745
Thermodynamic parameters to predict stability of RNA/DNA hybrid duplexes
Sugimoto, N., Nakano, S.-I., Katoh, M., Matsumura, A., Nakamuta, H., Ohmichi, T., Yoneyama, M., & Sasaki, M. (1995) · Biochemistry · doi:10.1021/bi00035a029 · Cited by 697 · cited 1×
“A proposed rule of thumb for designing the binding arms is that each arm should have a binding free energy (DG ) of at least 10– 12 kcal/mol (72), as calculated on the basis of tabulated RNA:DNA interaction energies (79).”
BackgroundLi, Y., & Breaker, R. R. (1999) · Journal of the American Chemical Society · doi:10.1021/ja990592p · Cited by 587
Cech, T. R. (2000) · Science · doi:10.1126/science.289.5481.878 · Cited by 382
Peptide bond formation by in vitro selected ribozymes
Zhang, B., & Cech, T. R. (1997) · Nature · doi:10.1038/36375 · Cited by 299
In vitro evolution suggests multiple origins for the hammerhead ribozyme
Salehi-Ashtiani, K., & Szostak, J. W. (2001) · Nature · doi:10.1038/35102081 · Cited by 183
Cited by
Liu, J., & Lu, Y. (2007) · Angewandte Chemie International Edition · doi:10.1002/anie.200702006 · Cited by 593
McConnell, E. M., Cozma, I., Mou, Q., Brennan, J. D., Lu, Y., & Li, Y. (2021) · Chemical Society Reviews · doi:10.1039/d1cs00240f · Cited by 477
Isaacs, F. J., Dwyer, D. J., & Collins, J. J. (2006) · Nature Biotechnology · doi:10.1038/nbt1208 · Cited by 373
Nucleic Acid Conjugated Nanomaterials for Enhanced Molecular Recognition
Wang, H., Yang, R., Yang, L., & Tan, W. (2009) · ACS Nano · doi:10.1021/nn9006303 · Cited by 329
A Highly Selective DNAzyme Sensor for Mercuric Ions
Hollenstein, M., Hipolito, C., Lam, C., Dietrich, D., & Perrin, D. M. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200800960 · Cited by 317
Metadata
- Authors
- Silverman, S. K.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-10-24
- Volume
- 33
- Issue
- 19
- Pages
- 6151-6163
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2142786354
- Cited by
- 303
- References
- 7
- FWCI
- 6.57
- Type
- journal article
- Language
- en
- Open access
- gold
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