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Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments
Open accessAbstract
The largely unused uracil-excision molecular cloning technique has excellent features in most aspects compared to other modern cloning techniques. Its application has, however, been hampered by incompatibility with proof-reading DNA polymerases. We have advanced the technique by identifying PfuCx as a compatible proof-reading DNA polymerase and by developing an improved vector design strategy. The original features of the technique, namely simplicity, speed, high efficiency and low cost are thus combined with high fidelity as well as a transparent, simple and flexible vector design. A comprehensive set of vectors has been constructed covering a wide range of different applications and their functionality has been confirmed.
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Cited by
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Self-citationRonda, C., Pedersen, L. E., Hansen, H. G., Kallehauge, T. B., Betenbaugh, M. J., Nielsen, A. T., & Kildegaard, H. F. (2014) · Biotechnology and Bioengineering · doi:10.1002/bit.25233 · Cited by 195
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Metadata
- Authors
- Nour-Eldin, H. H., Hansen, B. G., Nørholm, M. H. H., Jensen, J. K., & Halkier, B. A.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-09-25
- Volume
- 34
- Issue
- 18
- Pages
- e122-e122
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2161645265
- Cited by
- 467
- References
- 0
- FWCI
- 3.56
- Type
- journal article
- Language
- en
- Open access
- gold
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- Nour-Eldin, H. H., Hansen, B. G., Nørholm, M. H. H., Jensen, J. K., & Halkier, B. A. (2006). Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments. Nucleic Acids Research. https://doi.org/10.1093/nar/gkl635
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
