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Targeted high-throughput sequencing of tagged nucleic acid samples
Open accessAbstract
High-throughput 454 DNA sequencing technology allows much faster and more cost-effective sequencing than traditional Sanger sequencing. However, the technology imposes inherent limitations on the number of samples that can be processed in parallel. Here we introduce parallel tagged sequencing (PTS), a simple, inexpensive and flexible barcoding technique that can be used for parallel sequencing any number and type of double-stranded nucleic acid samples. We demonstrate that PTS is particularly powerful for sequencing contiguous DNA fragments such as mtDNA genomes: in theory as many as 250 mammalian mtDNA genomes can be sequenced in a single GS FLX run. PTS dramatically increases the sequencing throughput of samples in parallel and thus fully mobilizes the resources of the 454 technology for targeted sequencing.
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Cited by
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Kircher, M., Sawyer, S., & Meyer, M. (2012) · Nucleic Acids Research · doi:10.1093/nar/gkr771 · Cited by 1,106
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Metadata
- Authors
- Meyer, M., Stenzel, U., Myles, S., Prüfer, K., & Hofreiter, M.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-08
- Volume
- 35
- Issue
- 15
- Pages
- e97
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2095901358
- Cited by
- 194
- References
- 0
- FWCI
- 8.02
- Type
- journal article
- Language
- en
- Open access
- gold
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- Meyer, M., Stenzel, U., Myles, S., Prüfer, K., & Hofreiter, M. (2007). Targeted high-throughput sequencing of tagged nucleic acid samples. Nucleic Acids Research. https://doi.org/10.1093/nar/gkm566
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
