The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus
Open accessAbstract
Caulobacter crescentus is widely used as a powerful model system for the study of prokaryotic cell biology and development. Analysis of this organism is complicated by a limited selection of tools for genetic manipulation and inducible gene expression. This study reports the identification and functional characterization of a vanillate-regulated promoter (P(van)) which meets all requirements for application as a multi-purpose expression system in Caulobacter, thus complementing the established xylose-inducible system (P(xyl)). Furthermore, we introduce a newly constructed set of integrating and replicating shuttle vectors that considerably facilitate cell biological and physiological studies in Caulobacter. Based on different narrow and broad-host range replicons, they offer a wide choice of promoters, resistance genes, and fusion partners for the construction of fluorescently or affinity-tagged proteins. Since many of these constructs are also suitable for use in other bacteria, this work provides a comprehensive collection of tools that will enrich many areas of microbiological research.
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Cited by
Wilhelm, R. C., Singh, R., Eltis, L. D., & Mohn, W. W. (2019) · The ISME Journal · doi:10.1038/s41396-018-0279-6 · Cited by 391
Duerig, A., Abel, S., Folcher, M., Nicollier, M., Schwede, T., Amiot, N., Giese, B., & Jenal, U. (2009) · Genes & Development · doi:10.1101/gad.502409 · Cited by 311
Rapid range shifts and megafaunal extinctions associated with late Pleistocene climate change
Seersholm, F. V., Werndly, D. J., Grealy, A., Johnson, T., Keenan Early, E. M., Lundelius, E. L., Winsborough, B., Farr, G. E., Toomey, R., Hansen, A. J., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-16502-3 · Cited by 92
(2022) · doi:10.1371/journal.pgen.1010274 · Cited by 18
BR-bodies facilitate adaptive responses and survival during copper stress in Caulobacter crescentus
Passos, C., Tomares, D. T., Yassine, H., Schnorr, W. E., Hunter, H., Wolfe-Feichter, H. K., Velier, J., Dzurik, K. G., Grillo, J., Gega, A., et al. (2025) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2025.110648 · Cited by 6
Metadata
- Authors
- Thanbichler, M., Iniesta, A. A., & Shapiro, L.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-11-29
- Volume
- 35
- Issue
- 20
- Pages
- e137-e137
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2006591980
- Cited by
- 368
- References
- 44
- FWCI
- 4.52
- Type
- journal article
- Language
- en
- Open access
- gold
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- Thanbichler, M., Iniesta, A. A., & Shapiro, L. (2007). A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus. Nucleic Acids Research. https://doi.org/10.1093/nar/gkm818
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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