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Cited by 244HIV Research and TreatmentRNA Interference and Gene DeliveryRNA regulation and disease

Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid

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Yeung, Man Lung, Bennasser, Yamina, Watashi, Koichi, Le, Shu-Yun, Houzet, Laurent, Jeang, Kuan-Teh·2009·Nucleic Acids Research
DOI 10.1093/nar/gkp707PMC PMC2770672
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Abstract

Small non-coding RNAs of 18-25 nt in length can regulate gene expression through the RNA interference (RNAi) pathway. To characterize small RNAs in HIV-1-infected cells, we performed linker-ligated cloning followed by high-throughput pyrosequencing. Here, we report the composition of small RNAs in HIV-1 productively infected MT4 T-cells. We identified several HIV-1 small RNA clones and a highly abundant small 18-nt RNA that is antisense to the HIV-1 primer-binding site (PBS). This 18-nt RNA apparently originated from the dsRNA hybrid formed by the HIV-1 PBS and the 3' end of the human cellular tRNAlys3. It was found to associate with the Ago2 protein, suggesting its possible function in the cellular RNAi machinery for targeting HIV-1.

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  • An 18-nt ncRNA complementary to the PBS, termed PBSncRNA, was discovered in cells latently infected with HIV-1 and in HeLa cells transfected with HIV-1 proviral DNA.

    “An 18-nt ncRNA complementary to the PBS, termed PBSncRNA, was discovered in cells latently infected with HIV-1 and in HeLa cells transfected with HIV-1 proviral DNA (96).”

    From Role of host tRNAs and aminoacyl-tRNA synthetases in retroviral replication (2019) · cites this paper as [96]

  • This tRNA fragment may be generated by the host cell RNAi machinery and serve to down-regulate HIV-1 gene expression.

    “Thus, this tRNA fragment may be generated by the host cell RNAi machinery and serve to down-regulate HIV-1 gene expression (96).”

    From Role of host tRNAs and aminoacyl-tRNA synthetases in retroviral replication (2019) · cites this paper as [96]

  • PBS ncRNA is believed to be produced from an annealed PBS-tRNA Lys,3 duplex by dicer processing, a source different from that of tRFs expressed in uninfected cells.

    “However, it is believed that PBS ncRNA is produced from an annealed PBS-tRNA Lys,3 duplex by dicer processing (96), a source different from that of tRFs expressed in uninfected cells.”

    From Role of host tRNAs and aminoacyl-tRNA synthetases in retroviral replication (2019) · cites this paper as [96]

  • Some tRFs have regulatory functions in the replication cycle of retroelements, but the underlying mechanisms remain unclear.

    “Some tRFs have regulatory functions in the replication cycle of retroelements (95,96), but the underlying mechanisms remain unclear.”

    From Role of host tRNAs and aminoacyl-tRNA synthetases in retroviral replication (2019) · cites this paper as [96]

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Cited by

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Authors
Yeung, M. L., Bennasser, Y., Watashi, K., Le, S.-Y., Houzet, L., & Jeang, K.-T.
Affiliations
National Institutes of Health (US)
Corresponding author
Jeang, Kuan-Teh
ORCID
Venue
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Published
2009-09-03
Volume
37
Issue
19
Pages
6575-6586
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
DOI
10.1093/nar/gkp707
PMC
PMC2770672
OpenAlex
W2115688236
Cited by
244
References
2
Citations by year
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FWCI
5.26
Type
journal article
Language
en
Open access
Licence
Locations
  • Nucleic Acids Research (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • The HKU Scholars Hub (University of Hong Kong) (submittedVersion, cc-by-nc-nd)
Funders
  • National Institutes of Health
  • NIH Office of the Director
  • National Cancer Institute
  • National Institute of Allergy and Infectious Diseases
Topics
  • Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Keywords
HIV-1, small RNA, RNA interference, primer binding site, Argonaute 2
MeSH terms
Cell Line, Humans, RNA, Double-Stranded, RNA, Transfer, Lys, RNA, Viral, T-Lymphocytes, Virus Replication, HIV-1, Sequence Analysis, RNA, RNA, Untranslated

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