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Yeast targets for mRNA methylation
Open accessAbstract
N(6)-Methyladenosine (m(6)A) is a modified base present in the mRNA of all higher eukaryotes and in Saccharomyces cerevisiae, where there is an increase in m(6)A levels during sporulation. The methyltransferase, Ime4, is responsible for this modification and has a role in the initiation of meiosis. However, neither the function, nor the extent of distribution of this nucleotide modification is established. We demonstrate that in S. cerevisiae, substantial levels of internal adenosine methylation are present in the GpA context in mRNA from sporulating cells, which is consistent with the preferred methylation consensus of higher eukaryotes. Based upon our quantification data, every second transcript could contain one m(6)A during meiosis. As methylation is distributed across all mRNA size ranges, it is likely that m(6)A is not limited to a small population of messages. We developed a new antibody based method for identifying m(6)A containing messages, and using this method the transcripts of three key, early regulators of meiosis, IME1, IME2 and IME4 itself, were identified as being methylated. The position of m(6)A in IME2 was narrowed down to a region in the 3'-end. Methylation of these and other targets suggests mechanisms by which IME4 could control developmental choices leading to meiosis.
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Cited by
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Meyer, K. D., Saletore, Y., Zumbo, P., Elemento, O., Mason, C. E., & Jaffrey, S. R. (2012) · Cell · doi:10.1016/j.cell.2012.05.003 · Cited by 4,223
ALKBH5 Is a Mammalian RNA Demethylase that Impacts RNA Metabolism and Mouse Fertility
Zheng, G., Dahl, J. A., Niu, Y., Fedorcsak, P., Huang, C.-M., Li, C. J., Vågbø, C. B., Shi, Y., Wang, W.-L., Song, S.-H., et al. (2013) · Molecular Cell · doi:10.1016/j.molcel.2012.10.015 · Cited by 3,550
“In mammals, each mRNA contains, on average, 3–5 m(6)A modifications within a consensus sequence previously revealed as Pu[G > A]m(6)AC[A/C/U] (Bodi et al., 2010; Dominissini et al., 2012; Bokar, 2005; Harper et al., 1990; Meyer et al., 2012); experimental results indicate that only a portion of the putative methylatio…”
InfluentialMammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase
Ping, X.-L., Sun, B.-F., Wang, L., Xiao, W., Yang, X., Wang, W.-J., Adhikari, S., Shi, Y., Lv, Y., Chen, Y.-S., et al. (2014) · Cell Research · doi:10.1038/cr.2014.3 · Cited by 2,375
MODOMICS: a database of RNA modification pathways—2013 update
Machnicka, M. A., Milanowska, K., Osman Oglou, O., Purta, E., Kurkowska, M., Olchowik, A., Januszewski, W., Kalinowski, S., Dunin-Horkawicz, S., Rother, K. M., et al. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks1007 · Cited by 1,013
Wang, Y., Li, Y., Yue, M., Wang, J., Kumar, S., Wechsler-Reya, R. J., Zhang, Z., Ogawa, Y., Kellis, M., Duester, G., et al. (2018) · Nature Neuroscience · doi:10.1038/s41593-017-0057-1 · Cited by 438
Metadata
- Authors
- Bodi, Z., Button, J. D., Grierson, D., & Fray, R. G.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-04-26
- Volume
- 38
- Issue
- 16
- Pages
- 5327-5335
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2096867044
- Cited by
- 159
- References
- 0
- FWCI
- 3.54
- Type
- journal article
- Language
- en
- Open access
- gold
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