The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
iSpinach: a fluorogenic RNA aptamer optimized for in vitro applications
Open accessAbstract
Using random mutagenesis and high throughput screening by microfluidic-assisted In Vitro Compartmentalization, we report the isolation of an order of magnitude times brighter mutants of the light-up RNA aptamers Spinach that are far less salt-sensitive and with a much higher thermal stability than the parent molecule. Further engineering gave iSpinach, a molecule with folding and fluorescence properties surpassing those of all currently known aptamer based on the fluorogenic co-factor 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI). We illustrate the potential of iSpinach in a new sensitive and high throughput-compatible fluorogenic assay that measures co-transcriptionally the catalytic constant (kcat) of a model ribozyme.
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Filonov, G. S., Moon, J. D., Svensen, N., & Jaffrey, S. R. (2014) · Journal of the American Chemical Society · doi:10.1021/ja508478x · Cited by 780
Fluorescent monitoring of RNA assembly and processing using the split-spinach aptamer
Rogers, T. A., Andrews, G. E., Jaeger, L., & Grabow, W. W. (2015) · ACS Synthetic Biology · doi:10.1021/sb5000725 · Cited by 86
Cited by
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Specht, E. A., Braselmann, E., & Palmer, A. E. (2017) · Annual Review of Physiology · doi:10.1146/annurev-physiol-022516-034055 · Cited by 444
Development and future of droplet microfluidics
Nan, L., Zhang, H., Weitz, D. A., & Shum, H. C. (2024) · Lab on a Chip · doi:10.1039/d3lc00729d · Cited by 253
Metadata
- Authors
- Autour, A., Westhof, E., & Ryckelynck, M.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2016-03-01
- Volume
- 44
- Issue
- 6
- Pages
- 2491-2500
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2293575659
- Cited by
- 168
- References
- 2
- FWCI
- 9.18
- Type
- journal article
- Language
- en
- Open access
- gold
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