The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging
Open accessAbstract
Fluorescent tools have revolutionized our ability to probe biological dynamics, particularly at the cellular level. Fluorescent sensors have been developed on several platforms, utilizing either small-molecule dyes or fluorescent proteins, to monitor proteins, RNA, DNA, small molecules, and even cellular properties, such as pH and membrane potential. We briefly summarize the impressive history of tool development for these various applications and then discuss the most recent noteworthy developments in more detail. Particular emphasis is placed on tools suitable for single-cell analysis and especially live-cell imaging applications. Finally, we discuss prominent areas of need in future fluorescent tool development—specifically, advancing our capability to analyze and integrate the plethora of high-content data generated by fluorescence imaging.
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Cites
Ultrasensitive fluorescent proteins for imaging neuronal activity
Chen, T.-W., Wardill, T. J., Sun, Y., Pulver, S. R., Renninger, S. L., Baohan, A., Schreiter, E. R., Kerr, R. A., Orger, M. B., Jayaraman, V., et al. (2013) · Nature · doi:10.1038/nature12354 · Cited by 6,474
Global analysis of protein localization in budding yeast
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“Beginning in the mid-2000s, researchers began using GFP-tagged libraries to screen strains harboring fluorescent versions of nearly every known protein in the yeast genome (63).”
BackgroundSpatially resolved, highly multiplexed RNA profiling in single cells
Chen, K. H., Boettiger, A. N., Moffitt, J. R., Wang, S., & Zhuang, X. (2015) · Science · doi:10.1126/science.aaa6090 · Cited by 2,969
The Comet Assay for DNA Damage and Repair: Principles, Applications, and Limitations
Collins, A. R. (2004) · Molecular Biotechnology · doi:10.1385/mb:26:3:249 · Cited by 2,892
Imaging individual mRNA molecules using multiple singly labeled probes
Raj, A., van den Bogaard, P., Rifkin, S. A., van Oudenaarden, A., & Tyagi, S. (2008) · Nature Methods · doi:10.1038/nmeth.1253 · Cited by 2,028
Visualization of Single RNA Transcripts in Situ
Femino, A. M., Fay, F. S., Fogarty, K., & Singer, R. H. (1998) · Science · doi:10.1126/science.280.5363.585 · Cited by 1,504
Single-cell proteomic analysis of S. cerevisiae reveals the architecture of biological noise
Newman, J. R. S., Ghaemmaghami, S., Ihmels, J., Breslow, D. K., Noble, M., DeRisi, J. L., & Weissman, J. S. (2006) · Nature · doi:10.1038/nature04785 · Cited by 1,483
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Activity-Based Profiling of Proteases
Sanman, L. E., & Bogyo, M. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035352 · Cited by 330
Cited by
Gorin, G., Fang, M., Chari, T., & Pachter, L. (2022) · PLOS Computational Biology · doi:10.1371/journal.pcbi.1010492 · Cited by 186
A general approach to the design of high-performance near-infrared (NIR) D-π-A type fluorescent dyes
Luo, X., Li, J., Zhao, J., Gu, L., Qian, X., & Yang, Y. (2019) · Chinese Chemical Letters · doi:10.1016/j.cclet.2019.03.012 · Cited by 119
Metadata
- Authors
- Specht, E. A., Braselmann, E., & Palmer, A. E.
- Venue
- Annual Review of Physiology
- Publisher
- Annual Reviews
- Published
- 2017-02-10
- Volume
- 79
- Issue
- 1
- Pages
- 93-117
- ISSN
- 0066-4278 (ISSN-L)
- 1545-1585
- OpenAlex
- W2345179863
- Cited by
- 444
- References
- 9
- FWCI
- 2.95
- Type
- journal article
- Language
- en
- Open access
- green
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- Specht, E. A., Braselmann, E., & Palmer, A. E. (2017). A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging. Annual Review of Physiology. https://doi.org/10.1146/annurev-physiol-022516-034055
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