The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Modeling glioblastoma invasion using human brain organoids and single-cell transcriptomics
Open accessAbstract
Background: Glioblastoma (GBM) consists of devastating neoplasms with high invasive capacity, which have been difficult to study in vitro in a human-derived model system. Therapeutic progress is also limited by cellular heterogeneity within and between tumors, among other factors such as therapy resistance. To address these challenges, we present an experimental model using human cerebral organoids as a scaffold for patient-derived GBM cell invasion. Methods: This study combined tissue clearing and confocal microscopy with single-cell RNA sequencing of GBM cells before and after co-culture with organoid cells. Results: We show that tumor cells within organoids extend a network of long microtubes, recapitulating the in vivo behavior of GBM. Transcriptional changes implicated in the invasion process are coherent across patient samples, indicating that GBM cells reactively upregulate genes required for their dispersion. Potential interactions between GBM and organoid cells identified by an in silico receptor–ligand pairing screen suggest functional therapeutic targets. Conclusions: Taken together, our model has proven useful for studying GBM invasion and transcriptional heterogeneity in vitro, with applications for both pharmacological screens and patient-specific treatment selection on a time scale amenable to clinical practice.
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Massively parallel digital transcriptional profiling of single cells
Zheng, G. X. Y., Terry, J. M., Belgrader, P., Ryvkin, P., Bent, Z. W., Wilson, R., Ziraldo, S. B., Wheeler, T. D., McDermott, G. P., Zhu, J., et al. (2017) · Nature Communications · doi:10.1038/ncomms14049 · Cited by 7,353
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Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastoma
Patel, A. P., Tirosh, I., Trombetta, J. J., Shalek, A. K., Gillespie, S. M., Wakimoto, H., Cahill, D. P., Nahed, B. V., Curry, W. T., Martuza, R. L., et al. (2014) · Science · doi:10.1126/science.1254257 · Cited by 5,063
A draft network of ligand–receptor-mediated multicellular signalling in human
Ramilowski, J. A., Goldberg, T., Harshbarger, J., Kloppmann, E., Lizio, M., Satagopam, V. P., Itoh, M., Kawaji, H., Carninci, P., Rost, B., et al. (2015) · Nature Communications · doi:10.1038/ncomms8866 · Cited by 887
Glioblastoma Model Using Human Cerebral Organoids
Ogawa, J., Pao, G. M., Shokhirev, M. N., & Verma, I. M. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.03.105 · Cited by 391
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Organoids: The current status and biomedical applications
Yang, S., Hu, H., Kung, H., Zou, R., Dai, Y., Hu, Y., Wang, T., Lv, T., Yu, J., & Li, F. (2023) · MedComm · doi:10.1002/mco2.274 · Cited by 266
Tumor Cell Infiltration into the Brain in Glioblastoma: From Mechanisms to Clinical Perspectives
Seker-Polat, F., Pinarbasi Degirmenci, N., Solaroglu, I., & Bagci-Onder, T. (2022) · Cancers · doi:10.3390/cancers14020443 · Cited by 253
Chen, C., Rengarajan, V., Kjar, A., & Huang, Y. (2021) · Bioactive Materials · doi:10.1016/j.bioactmat.2020.10.003 · Cited by 77
Present Application and Perspectives of Organoid Imaging Technology
Fei, K., Zhang, J., Yuan, J., & Xiao, P. (2022) · Bioengineering · doi:10.3390/bioengineering9030121 · Cited by 65
Glioblastoma-cortical organoids recapitulate cell state heterogeneity and intercellular transfer
Mangena, V., Chanoch-Myers, R., Sartore, R., Paulsen, B., Gritsch, S., Weisman, H., Hara, T., Breakefield, X. O., Breyne, K., Regev, A., et al. (2025) · Cancer Discovery · doi:10.1158/2159-8290.cd-23-1336 · Cited by 49
Metadata
- Authors
- Krieger, T. G., Tirier, S. M., Park, J., Jechow, K., Eisemann, T., Peterziel, H., Angel, P., Eils, R., & Conrad, C.
- Venue
- Neuro-Oncology
- Publisher
- Oxford University Press (OUP)
- Published
- 2020-04-16
- Volume
- 22
- Issue
- 8
- Pages
- 1138-1149
- ISSN
- 1522-8517 (ISSN-L)
- 1523-5866
- OpenAlex
- W3016319591
- Cited by
- 181
- References
- 7
- FWCI
- 8.42
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Krieger, T. G., Tirier, S. M., Park, J., Jechow, K., Eisemann, T., Peterziel, H., Angel, P., Eils, R., & Conrad, C. (2020). Modeling glioblastoma invasion using human brain organoids and single-cell transcriptomics. Neuro-Oncology. https://doi.org/10.1093/neuonc/noaa091
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