Glutathione Reductase in the Sea Urchin Egg
Abstract
The G-200 flow-through fraction of the extract of sea urchin eggs contained a complex form of glutathione reductase (GR) [EC 1.6.4.2]. The complex was unstable and gradually dissociated with ain increase in GR activity. The activation was facilitated by high concentrations of EDTA, KCI or (NH4)2SO4. The rate of activation by salts was apparently dependent on the ionic strength. The complex form was also activated rather quickly by treatment with proteinases such as trypsin [EC 3.4.21.4], alpha-chymotrypsin [EC 3.4.21.1] or subtilisin [EC 3.4.21.14]. Trypsin caused the complex to release the free form of GR. Even after trypsin treatment, little change was observed in the dependence of the GR activity on GSSG or NADPH concentration. The GR activity of the complex form was not inhibited at all by 0.2 mM N-ethylmaleimide (NEM) in the presence of GSSG, but was reduced to 3% in the presence of NADPH. When excess NEM was sequestered with GSH, the NEM-treated complex form was strikingly activated by trypsin, while no activation was detected with the free form of enzyme pretreated with NEM. These results suggest that the active site of GR in the complex form is largely masked by a polypeptide moiety of theinhbitiory component.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
GSR (SPU_025989) is expressed at high levels during development.
“GSR (SPU_025989) is also expressed at high levels during development, as has been known for some time (Ichio, 1977;Ii and Sakai, 1974a,b).”
From The chemical defensome: Environmental sensing and response genes in the Strongylocentrotus purpuratus genome (2006) · cites this paper as Ichio, 1977
Cited by
Sorted by citations
Goldstone, J. V., Hamdoun, A., Cole, B. J., Howard-Ashby, M., Nebert, D. W., Scally, M., Dean, M., Epel, D., Hahn, M. E., & Stegeman, J. J. (2006) · Developmental Biology · doi:10.1016/j.ydbio.2006.08.066 · Cited by 280 · see citing works
“GSR (SPU_025989) is also expressed at high levels during development, as has been known for some time (Ichio, 1977; Ii and Sakai, 1974a,b).”
BackgroundMouse‐Liver Glutathione Reductase
López‐Barea, J., & Lee, C. (1979) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1979.tb13210.x · Cited by 117 · see citing works
Färber, P. M., Becker, K., Müller, S., et al. (1996) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1996.0655u.x · Cited by 54 · see citing works
Roth, Z., Chayen, N. E., & Dikstein, S. (1983) · International review of cytology · doi:10.1016/s0074-7696(08)62369-4 · Cited by 13 · see citing works
Glutathione peroxidases and glutathione reductase activities during Bufo bufo development
Ilio, C. D., Boccio, G. D., Miranda, M., et al. (1986) · Comparative Biochemistry and Physiology Part C Comparative Pharmacology · doi:10.1016/0742-8413(86)90004-6 · Cited by 7 · see citing works
Nardi, G., & Cipollaro, M. (1988) · Development Growth & Differentiation · doi:10.1111/j.1440-169x.1988.00383.x · Cited by 6 · see citing works
Rebhun, L. I. (1978) · Elsevier eBooks · doi:10.1016/b978-0-12-217850-4.50053-8 · Cited by 2 · see citing works
- Authors
- Ichio
- Affiliations
- The University of Tokyo (JP)
- Corresponding author
- Ichio
- Venue
- The Journal of Biochemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1977-03
- Volume
- 81
- Issue
- 3
- Pages
- 673-680
- ISSN
- 0021-924X (ISSN-L)
- 1756-2651
- OpenAlex
- W1933320676
- Cited by
- 7
- References
- 0
- FWCI
- 0.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glutathione Transferases and Polymorphisms
- Health Sciences › Nursing › Nutrition and Dietetics › Selenium in Biological Systems
- Keywords
- glutathione reductase, sea urchin eggs, enzyme complex, enzyme activation, trypsin, protease activation, N-ethylmaleimide, ionic strength
- MeSH terms
- Animals, Binding Sites, Chymotrypsin, Enzyme Activation, Ethylmaleimide, Female, Glutathione, Glutathione Reductase, Kinetics, NADP, Ovum, Oxidation-Reduction, Peptide Hydrolases, Sea Urchins, Subtilisins, Trypsin
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
