Gene expression activity and pathway selection for sucrose metabolism in developing storage root of sweet potato
Abstract
Development of sweet potato (Ipomoea batatas) storage root coincides with starch accumulation made using cleaved products of imported photoassimilate sucrose. The genes and pathways are predominantly active for sucrose metabolism in developing storage root were unknown. In this study, we used both an expressed sequence tag (EST) approach and a reverse transcription-polymerase chain reaction (RT-PCR) approach to answer this question. Sucrose synthase (SuSy) was found to be significantly more frequent in storage root ESTs than in fibrous root ESTs. SuSy was the most abundant carbohydrate-metabolism gene in the storage-root ESTs. RT-PCR results confirmed this by showing that invertase was active in fibrous roots but rapidly decreased to an undetectable level during storage root development while SuSy became predominant. Invertase expression was also detectable in young immature storage root and shoot tips, suggesting an involvement in cell formation. SuSy expression pattern showed considerable similarity to that of ADP-glucose pyrophosphorylase, an essential enzyme for starch synthesis. The results indicated that (i). SuSy was the most actively expressed enzyme in sucrose metabolism in developing storage root and was correlated with sink strength, and (ii). whereas invertase was active at cell formation stages, SuSy pathway was predominant for sucrose cleavage related to starch-accumulation.
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The dry matter content (ranging from 18 to 42%) and starch content of storage roots varies greatly among sweet potato genotypes.
“Furthermore, the dry matter content (ranging from 18 to 42%) and starch content of the SRs varies greatly among sweet potato genotypes (Li and Zhang, 2003; Ravi et al., 2009; Tumwegamire et al., 2011)”
From Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato (2017) · cites this paper as Li and Zhang, 2003
In storage roots, starch is synthesized from the cleaved products of imported photoassimilate sucrose from photosynthetic organs.
“In SRs, starch is synthesized from the cleaved products of imported photoassimilate sucrose from photosynthetic organs (Li and Zhang, 2003).”
From Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato (2017) · cites this paper as Li and Zhang, 2003
Starch is synthesized in plant storage organs using the cleaved products of sucrose, which is the main photoassimilate from photosynthetic organs.
“Starch is synthesized in plant storage organs using the cleaved products of sucrose, which is the main photoassimilate from photosynthetic organs (Li and Zhang, 2003).”
From Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato (2017) · cites this paper as Li and Zhang, 2003
The SuSy pathway was shown to be the predominant pathway underlying sucrose cleavage related to starch accumulation in sweet potato.
“The SuSy pathway was shown to be the predominant pathway underlying sucrose cleavage related to starch accumulation in sweet potato (Li and Zhang, 2003).”
From Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato (2017) · cites this paper as Li and Zhang, 2003
Two pathways mediate sucrose cleavage in the cytosol; in one, sucrose is converted to glucose and fructose by invertase, and in the other, sucrose is converted to UDP-glucose and fructose by SuSy, and the UDP-glucose is then converted to glucose-1-phosphate by UGPase.
“Two pathways mediate sucrose cleavage in the cytosol; in one, sucrose is converted to glucose and fructose by invertase, and in the other, sucrose is converted to UDP-glucose and fructose by SuSy (Li and Zhang, 2003; Wind et al., 2010), and the UDP-glucose is then converted to glucose-1-phosphate by UGPase, for use in subsequent reactions related to starch synthesis (Figure S2; Li and Zhang, 2003;”
From Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato (2017) · cites this paper as Li and Zhang, 2003
In sweet potato storage roots, starch is mainly synthesized from sucrose produced from photosynthesis.
“In sweet potato storage roots, starch is mainly synthesized from sucrose that is produced from photosynthesis [25].”
From RNA-Seq and iTRAQ reveal multiple pathways involved in storage root formation and development in sweet potato (Ipomoea batatas L.) (2019) · cites this paper as [25]
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- Authors
- Li, X.-Q., & Zhang, D.
- Affiliations
- Agriculture and Agri-Food Canada (CA)
- International Potato Center (PE)
- Venue
- Plant and Cell Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-06-15
- Volume
- 44
- Issue
- 6
- Pages
- 630-636
- ISSN
- 0032-0781 (ISSN-L)
- 1471-9053
- OpenAlex
- W2161904346
- Cited by
- 89
- References
- 32
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 11 · 2023: 6 · 2022: 2 · 2021: 5 · 2020: 5 · 2019: 8 · 2018: 6 · 2017: 5 · 2016: 7 · 2015: 4 · 2014: 3 · 2013: 3 · 2012: 3
- FWCI
- 2.75
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Plant and Cell Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Agriculture and Agri-Food Canada
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Postharvest Quality and Shelf Life Management
- Keywords
- Ipomoea batatas, storage root development, sucrose metabolism, sucrose synthase, invertase, ADP-glucose pyrophosphorylase, starch accumulation, gene expression, expressed sequence tags, sink strength, fibrous roots, cell formation
- MeSH terms
- Base Sequence, Cell Differentiation, Glucosyltransferases, Glycoside Hydrolases, Molecular Sequence Data, Nucleotidyltransferases, RNA, Messenger, Starch, Sucrose, Genomic Library, Gene Expression Regulation, Enzymologic, DNA, Complementary, Gene Expression Regulation, Plant, Plant Roots, Plant Shoots, Genome, Plant, Ipomoea, beta-Fructofuranosidase, Glucose-1-Phosphate Adenylyltransferase
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