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Cited by 28Light effects on plantsAlgal biology and biofuel productionPhotoreceptor and optogenetics research

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Aureochromes maintain polyunsaturated fatty acid content in Nannochloropsis oceanica

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Poliner, Eric, Busch, Andrea W U, Newton, Linsey, Kim, Young Uk, Clark, Rachel, Gonzalez-Martinez, Sofía C, Jeong, Byeong-Ryool, Montgomery, Beronda L, Farré, Eva M·2022·Plant Physiology
DOI 10.1093/plphys/kiac052PMC PMC9157131
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Abstract

Nannochloropsis oceanica, like other stramenopile microalgae, is rich in long-chain polyunsaturated fatty acids (LC-PUFAs) such as eicosapentaenoic acid (EPA). We observed that fatty acid desaturases (FADs) involved in LC-PUFA biosynthesis were among the strongest blue light-induced genes in N. oceanica CCMP1779. Blue light was also necessary for maintaining LC-PUFA levels in CCMP1779 cells, and growth under red light led to a reduction in EPA content. Aureochromes are stramenopile-specific proteins that contain a light–oxygen–voltage (LOV)-sensing domain that associates with a flavin mononucleotide and is able to sense blue light. These proteins also contain a basic leucine zipper DNA-binding motif and can act as blue light-regulated transcription factors by associating with an E-box like motif, which we found enriched in the promoters of blue light-induced genes. We demonstrated that, in vitro, two CCMP1779 aureochromes were able to absorb blue light. Moreover, the loss or reduction of the expression of any of the three aureochrome genes led to a decrease in the blue light-specific induction of several FADs in CCMP1779. EPA content was also significantly reduced in NoAUREO2 and NoAUREO4 mutants. Taken together, our results indicate that aureochromes mediate blue light-dependent regulation of LC-PUFA content in N. oceanica CCMP1779 cells.

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  • Several studies have investigated the impact of LED light on the enhancement of lipids in microalgae, highlighting the significant role of blue light.

    “Several studies have specifically investigated the impact of LED light on the enhancement of lipids in microalgae, highlighting the significant role of blue light in this process [137, 145, 146].”

    From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [146]

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Metadata

Authors
Poliner, E., Busch, A. W. U., Newton, L., Kim, Y. U., Clark, R., Gonzalez-Martinez, S. C., Jeong, B.-R., Montgomery, B. L., & Farré, E. M.
Affiliations
  • Michigan State University (US)
  • Korea Advanced Institute of Science and Technology (KR)
  • Chinese Academy of Sciences (CN)
Corresponding author
Farré, Eva M
ORCID
Venue
Plant Physiology
Publisher
Oxford University Press (OUP)
Published
2022-02-15
Volume
189
Issue
2
Pages
906-921
ISSN
  • 0032-0889 (ISSN-L)
  • 1532-2548
DOI
10.1093/plphys/kiac052
PMC
PMC9157131
OpenAlex
W4211032254
Cited by
28
References
1
Citations by year
2026: 5 · 2025: 3 · 2024: 11 · 2023: 5 · 2022: 4
FWCI
3.17
Type
journal article
Language
en
Open access
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations
  • PLANT PHYSIOLOGY (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Scholarworks@UNIST (Ulsan National Institute of Science and Technology) (submittedVersion)
Funders
  • Nationa Science Foundation (IOS-1354721)
  • Michigan State University
  • US Department of Energy (DE-FG02–91ER20021)
  • PlantGenomics@MSU program [NSF (DBI-1358474)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
Keywords
Nannochloropsis oceanica, long-chain polyunsaturated fatty acids, eicosapentaenoic acid, fatty acid desaturase, blue light signaling, LOV domain, transcription factors, red light
MeSH terms
Fatty Acids, Fatty Acids, Unsaturated, Light, Eicosapentaenoic Acid, Fatty Acid Desaturases, Stramenopiles, Microalgae

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