TNF-α-induced aquaporin 9 in synoviocytes from patients with OA and RA
Abstract
OBJECTIVES: To determine whether aquaporins (AQPs) are expressed in the synovial tissues of patients with OA and RA, and to examine the patterns of expression in patients with and without hydrarthrosis. METHODS: AQPs were detected in synovial tissue samples from patients with OA and RA using RT-PCR and immunohistochemistry. Fibroblast-like synoviocytes (FLSs) from patients with OA and RA were cultured and stimulated with TNF-alpha. The expression of AQPs in FLSs was examined using RT-PCR and western blot analyses and the function of aquaglyceroporins was examined by a glycerol uptake assay. RESULTS: AQP1, -3 and -9 mRNAs were expressed in synovial tissues from patients with OA and RA. AQP1, -3 and -9 proteins were also detected by immunohistochemistry. AQP9 mRNA was expressed more strongly in the synovial tissues of OA patients with hydrarthrosis than those without. AQP9 mRNA and protein expression were strongly induced with TNF-alpha treatment in FLSs, whereas the expression of AQP1 and -3 mRNAs was not induced with TNF-alpha treatment. AQP9 as an aquaglyceroporin was induced by TNF-alpha. CONCLUSIONS: AQP9 mRNA was detected in synovial tissues from OA and RA patients with hydrarthrosis. AQP9 expression was strongly induced in FLSs with TNF-alpha. Although the functions of AQP1, -3 and -9 in synovial tissues remain to be elucidated, it suggested that AQP9 might be related to the pathogenesis of hydrarthrosis and inflammatory synovitis.
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High expression of AQP9 was detected in synovial tissues and fibroblast-like synoviocytes from osteoarthritis and rheumatoid arthritis patients and may have a role in the pathogenesis of inflammatory synovitis.
“A high expression of this transporter was detected in synovial tissues and fibroblast-like synoviocytes from osteoarthritis and rheumatoid arthritis patients and may have a role in the pathogenesis of inflammatory synovitis [145,146].”
From Cellular and Molecular Mechanisms in the Pathogenesis of Classical, Vascular, and Hypermobile Ehlers‒Danlos Syndromes (2019) · cites this paper as [145]
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- Authors
- Nagahara, M., Waguri-Nagaya, Y., Yamagami, T., Aoyama, M., Tada, T., Inoue, K., Asai, K., & Otsuka, T.
- Affiliations
- Nagoya City University (JP)
- Venue
- Rheumatology
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-02-24
- Volume
- 49
- Issue
- 5
- Pages
- 898-906
- ISSN
- 1462-0324 (ISSN-L)
- 1462-0332
- OpenAlex
- W2135599760
- Cited by
- 36
- References
- 43
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 3 · 2022: 5 · 2020: 3 · 2019: 7 · 2018: 3 · 2017: 2 · 2016: 2 · 2015: 2 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 0.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Lara D. Veeken (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
- Health Sciences › Medicine › Rheumatology › Rheumatoid Arthritis Research and Therapies
- Keywords
- AQP9, aquaporins, TNF-α, synovial fibroblasts, osteoarthritis, rheumatoid arthritis, joint effusion, synovium, glycerol uptake, aquaglyceroporins, aquaporin-1, AQP3
- MeSH terms
- Aged, Aged, 80 and over, Arthritis, Arthritis, Rheumatoid, Cells, Cultured, Female, Humans, Hydrarthrosis, Knee Joint, Male, Middle Aged, Osteoarthritis, Synovial Membrane, Synovitis, Tumor Necrosis Factor-alpha, Aquaporins
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