The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Novel Aspects of Tomato Root Colonization and Infection by Fusarium oxysporum f. sp. radicis-lycopersici Revealed by Confocal Laser Scanning Microscopic Analysis Using the Green Fluorescent Protein as a Marker
Open accessAbstract
The fungus Fusarium oxysporum f. sp. radicis-lycopersici is the causal agent of tomato foot and root rot disease. The green fluorescent protein (GFP) was used to mark this fungus in order to visualize and analyze the colonization and infection processes in vivo. Transformation of F. oxysporum f. sp. radicis-lycopersici was very efficient and gfp expression was stable for at least nine subcultures. Microscopic analysis of the transformants revealed homogeneity of the fluorescent signal, which was clearly visible in the hyphae as well as in the chlamydospores and conidia. To our knowledge, this is the first report in which this is shown. The transformation did not affect the pathogenicity. Using confocal laser scanning microscopy, colonization, infection, and disease development on tomato roots were visualized in detail and several new aspects of these processes were observed, such as (i) the complete colonization pattern of the tomato root system; (ii) the very first steps of contact between the fungus and the host, which takes place at the root hair zone by mingling and by the attachment of hyphae to the root hairs; (iii) the preferential colonization sites on the root surface, which are the grooves along the junctions of the epidermal cells; and (iv) the absence of specific infection sites, such as sites of emergence of secondary roots, root tips, or wounded tissue, and the absence of specific infection structures, such as appressoria. The results of this work prove that the use of GFP as a marker for F. oxysporum f. sp. radicis-lycopersici is a convenient, fast, and effective approach for studying plant-fungus interactions.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Tsien, R. Y. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.509 · Cited by 6,143
Engineered GFP as a vital reporter in plants
Chiu, W.-L., Niwa, Y., Zeng, W., Hirano, T., Kobayashi, H., & Sheen, J. (1996) · Current Biology · doi:10.1016/s0960-9822(02)00483-9 · Cited by 1,364
Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli
Punt, P. J., Oliver, R. P., Dingemanse, M. A., Pouwels, P. H., & van den Hondel, C. A. M. J. J. (1987) · Gene · doi:10.1016/0378-1119(87)90164-8 · Cited by 937
Self-citationM OLECULAR D ETERMINANTS OF R HIZOSPHERE C OLONIZATION BY P SEUDOMONAS
Lugtenberg, B. J. J., Dekkers, L., & Bloemberg, G. V. (2001) · Annual Review of Phytopathology · doi:10.1146/annurev.phyto.39.1.461 · Cited by 804
Self-citationMethods for Research on Soilborne Phytopathogenic Fungi
Bills, G. F., Singleton, L. L., Mihail, J. D., & Rush, C. M. (1993) · Mycologia · doi:10.2307/3760494 · Cited by 622
Solubilization of rock phosphate by rape
Hoffland, E., Findenegg, G. R., & Nelemans, J. A. (1989) · Plant and Soil · doi:10.1007/bf02280176 · Cited by 493
Simons, M. (1996) · Molecular Plant-Microbe Interactions · doi:10.1094/mpmi-9-0600 · Cited by 396
Chin-A-Woeng, T. F. C., Bloemberg, G. V., van der Bij, A. J., van der Drift, K. M. G. M., Schripsema, J., Kroon, B., Scheffer, R. J., Keel, C., Bakker, P. A. H. M., Tichy, H.-V., et al. (1998) · Molecular Plant-Microbe Interactions® · doi:10.1094/mpmi.1998.11.11.1069 · Cited by 363
Self-citationGreen Fluorescent Protein Is Lighting Up Fungal Biology
Lorang, J. M., Tuori, R. P., Martinez, J. P., Sawyer, T., Redman, R. S., Rollins, J. A., Wolpert, T., Johnson, K. B., Rodriguez, R. J., Dickman, M. B., et al. (2001) · Applied and Environmental Microbiology · doi:10.1128/aem.67.5.1987-1994.2001 · Cited by 327
Cited by
Pathogen profile update: Fusarium oxysporum
MICHIELSE, C. B., & REP, M. (2009) · Molecular Plant Pathology · doi:10.1111/j.1364-3703.2009.00538.x · Cited by 786
Fusarium oxysporum : exploring the molecular arsenal of a vascular wilt fungus
Pietro, A. D., Madrid, M. P., Caracuel, Z., Delgado‐Jarana, J., & Roncero, M. I. G. (2003) · Molecular Plant Pathology · doi:10.1046/j.1364-3703.2003.00180.x · Cited by 464
Phenazines and their role in biocontrol by Pseudomonas bacteria
Chin‐A‐Woeng, T. F. C., Bloemberg, G. V., & Lugtenberg, B. J. J. (2003) · New Phytologist · doi:10.1046/j.1469-8137.2003.00686.x · Cited by 375
Self-citationKhan, N., Martínez-Hidalgo, P., Ice, T. A., Maymon, M., Humm, E. A., Nejat, N., Sanders, E. R., Kaplan, D., & Hirsch, A. M. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02363 · Cited by 282
Bleichrodt, R.-J., Vinck, A., Read, N. D., & Wösten, H. A. B. (2015) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2015.06.010 · Cited by 38
“The cloned fragment was cut from the resulting vector pRB068 with NcoI/HindIII and ligated in pGPDGFP (Lagopodi et al., 2002) that had been digested with the same enzymes.”
Method
Metadata
- Authors
- Lagopodi, A. L., Ram, A. F. J., Lamers, G. E. M., Punt, P. J., Van den Hondel, C. A. M. J. J., Lugtenberg, B. J. J., & Bloemberg, G. V.
- Venue
- Molecular Plant-Microbe Interactions®
- Publisher
- Scientific Societies
- Published
- 2002-02
- Volume
- 15
- Issue
- 2
- Pages
- 172-179
- ISSN
- 0894-0282 (ISSN-L)
- 1943-7706
- OpenAlex
- W2021079617
- Cited by
- 269
- References
- 16
- FWCI
- 4.00
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Novel Aspects of Tomato Root Colonization and Infection by Fusarium oxysporum f. sp. radicis-lycopersici Revealed by Confocal Laser Scanning Microscopic Analysis Using the Green Fluorescent Protein as a Marker. https://app.cochat.ai/papers/10.1094/mpmi.2002.15.2.172
- The paper
- Lagopodi, A. L., Ram, A. F. J., Lamers, G. E. M., Punt, P. J., Van den Hondel, C. A. M. J. J., Lugtenberg, B. J. J., & Bloemberg, G. V. (2002). Novel Aspects of Tomato Root Colonization and Infection by Fusarium oxysporum f. sp. radicis-lycopersici Revealed by Confocal Laser Scanning Microscopic Analysis Using the Green Fluorescent Protein as a Marker. Molecular Plant-Microbe Interactions®. https://doi.org/10.1094/mpmi.2002.15.2.172
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
