Silicon Enhances the Accumulation of Diterpenoid Phytoalexins in Rice: A Potential Mechanism for Blast Resistance

Abstract

Although several reports underscore the importance of silicon (Si) in controlling Magnaporthe grisea on rice, no study has associated this beneficial effect with specific mechanisms of host defense responses against this fungal attack. In this study, however, we provide evidence that higher levels of momilactone phytoalexins were found in leaf extracts from plants inoculated with M. grisea and amended with silicon (Si+) than in leaf extracts from inoculated plants not amended with silicon (Si- ) or noninoculated Si+ and Si- plants. On this basis, the more efficient stimulation of the terpenoid pathway in Si+ plants and, consequently, the increase in the levels of momilactones appears to be a factor contributing to enhanced rice resistance to blast. This may explain the lower level of blast severity observed on leaves of Si+ plants at 96 h after inoculation with M. grisea. The results of this study strongly suggest that Si plays an active role in the resistance of rice to blast rather than the formation of a physical barrier to penetration by M. grisea.