The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Glutathione Concentration during Maturation and after Fertilization in Pig Oocytes: Relevance to the Ability of Oocytes to Form Male Pronucleus1
Open accessAbstract
The present study examined the kinetics of glutathione (GSH) concentration during maturation and after fertilization in pig oocytes and its relevance to the ability of pig oocytes to form a male pronucleus after in vitro fertilization. The GSH concentration was significantly higher in pig oocytes matured in Waymouth medium than in pig oocytes matured in either modified (m) TCM-199 or mTLP media. The addition of 0.04-0.57 mM cysteine (CySH) to mTLP significantly increased both the GSH concentrations in oocytes matured in vitro and the rate of male pronucleus formation as compared to those in oocytes cultured in mTLP alone. When pig oocytes were cultured 12, 24, or 36 h in mTLP plus 0.14 mM CySH, their GSH concentrations were significantly higher than in uncultured oocytes. After fertilization, the GSH concentration in pig oocytes declined significantly. GSH concentrations in oocytes matured in vivo did not differ from those in oocytes matured in mTLP plus 0.14 or 0.57 mM CySH. The results indicate that 1) the composition of maturation medium affects the GSH concentration in pig oocytes; 2) the addition of CySH to maturation medium permits GSH synthesis by the pig oocytes; 3) GSH levels in pig oocytes change during maturation and after fertilization; and 4) GSH synthesis during oocyte maturation is an important factor for promoting their ability to form a male pronucleus after fertilization.
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Citations
Cites
Tietze, F. (1969) · Analytical Biochemistry · doi:10.1016/0003-2697(69)90064-5 · Cited by 6,047
Selective Modification of Glutathione Metabolism
Meister, A. (1983) · Science · doi:10.1126/science.6836290 · Cited by 1,267 · cited 1×
“[4] that GSH levels in hamster oocytes change during maturation and fertilization and the finding of Meister [26] that intracellular concentrations of GSH are dependent on the availability of CySH.”
BackgroundThe Glutathione Status of Cells
Kosower, N. S., & Kosower, E. M. (1978) · International Review of Cytology · doi:10.1016/s0074-7696(08)60166-7 · Cited by 1,211
Nutrition of Animal Cells in Tissue Culture. I. Initial Studies on a Synthetic Medium.,
Morgan, J. F., Morton, H. J., & Parker, R. C. (1950) · Experimental Biology and Medicine · doi:10.3181/00379727-73-17557 · Cited by 1,049
Perreault, S. D., Barbee, R. R., & Slott, V. L. (1988) · Developmental Biology · doi:10.1016/0012-1606(88)90070-x · Cited by 328
Waymouth, C. (1959) · JNCI: Journal of the National Cancer Institute · doi:10.1093/jnci/22.5.1003 · Cited by 323
Electron microscope studies of sperm incorporation into the golden hamster egg
Yanagimachi, R., & Noda, Y. D. (1970) · American Journal of Anatomy · doi:10.1002/aja.1001280404 · Cited by 222
The role of disulfide bond reduction during mammalian sperm nuclear decondensation in vivo
Perreault, S. D., Wolff, R. A., & Zirkin, B. R. (1984) · Developmental Biology · doi:10.1016/0012-1606(84)90126-x · Cited by 219
Regulation of 'haploid expressed genes' in male germ cells
Hecht, N. B. (1990) · Reproduction · doi:10.1530/jrf.0.0880679 · Cited by 174
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Cited by
The importance of folate, zinc and antioxidants in the pathogenesis and prevention of subfertility
Ebisch, I. M. W., Thomas, C. M. G., Peters, W. H. M., Braat, D. D. M., & Steegers-Theunissen, R. P. M. (2007) · Human Reproduction Update · doi:10.1093/humupd/dml054 · Cited by 503
Production of cloned pigs from in vitro systems
Betthauser, J., Forsberg, E., Augenstein, M., Childs, L., Eilertsen, K., Enos, J., Forsythe, T., Golueke, P., Jurgella, G., Koppang, R., et al. (2000) · Nature Biotechnology · doi:10.1038/80242 · Cited by 489
Sutton, M. L. (2003) · Human Reproduction Update · doi:10.1093/humupd/dmg009 · Cited by 406
Oxidative stress and antioxidants: exposure and impact on female fertility
Ruder, E. H., Hartman, T. J., Blumberg, J., & Goldman, M. B. (2008) · Human Reproduction Update · doi:10.1093/humupd/dmn011 · Cited by 351
Metadata
- Authors
- Yoshida, M., Ishigaki, K., Nagai, T., Chikyu, M., & Pursel, V. G.
- Venue
- Biology of Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 1993-07-01
- Volume
- 49
- Issue
- 1
- Pages
- 89-94
- ISSN
- 0006-3363 (ISSN-L)
- 1529-7268
- OpenAlex
- W2120322690
- Cited by
- 373
- References
- 13
- FWCI
- 13.12
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Yoshida, M., Ishigaki, K., Nagai, T., Chikyu, M., & Pursel, V. G. (1993). Glutathione Concentration during Maturation and after Fertilization in Pig Oocytes: Relevance to the Ability of Oocytes to Form Male Pronucleus1. Biology of Reproduction. https://doi.org/10.1095/biolreprod49.1.89
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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