The presence of nitrite during UVA irradiation protects from apoptosis
Abstract
Nitrite occurs ubiquitously in biological fluids such as blood and sweat, representing an oxidation product of nitric oxide. Nitrite has been associated with a variety of adverse effects such as mutagenicity, carcinogenesis, and toxicity. In contrast, here we demonstrate that the presence of nitrite, but not nitrate, during irradiation of endothelial cells in culture exerts a potent and concentration-dependent protection against UVA-induced apoptotic cell death. Protection is half-maximal at a concentration of 3 mM, and complete rescue is observed at 10 mM. Nitrite-mediated protection is mediated via inhibition of lipid peroxidation in a similar manner as seen with butylated hydroxytoluene, a known inhibitor of lipid peroxidation. Interestingly, nitrite-mediated protection is completely abolished by coincubation with the NO scavenger cPTIO. Using electron paramagnetic resonance (EPR) spectroscopy or Faraday modulation spectroscopy, we directly prove UVA-induced NO formation in solutions containing nitrite. In conclusion, evidence is presented that nitrite represents a protective agent against UVA-induced apoptosis due to photodecomposition of nitrite and subsequent formation of NO.
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The presence of nitrite, but not nitrate, reduced the extent of apoptosis in cultured endothelial cells during UVA-irradiation in a concentration-dependent manner by inhibiting lipid peroxidation; this protective effect was abolished by simultaneous administration of a NO scavenger.
“Later on, the presence of nitrite, but not nitrate, reduced the extent of apoptosis in cultured endothelial cells during UVA-irradiation in a concentration-dependent manner by inhibiting lipid peroxidation; this protective effect was abolished by simultaneous administration of a NO scavenger (Suschek et al., 2003)”
From Nitrite in organ protection (2014) · cites this paper as Suschek et al., 2003
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- Authors
- Suschek, C. V., Schroeder, P., Aust, O., Sies, H., Mahotka, C., Horstjann, M., Ganser, H., Mürtz, M., Hering, P., Schnorr, O., Kröncke, K., & Kolb‐Bachofen, V.
- Affiliations
- Heinrich Heine University Düsseldorf (DE)
- Institute of Biochemistry and Molecular Biology I Heinrich‐Heine‐University of Düsseldorf 40225 Düsseldorf Germany
- Department of Immunobiology Heinrich‐Heine‐University of Düsseldorf 40225 Düsseldorf Germany
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2003-10-02
- Volume
- 17
- Issue
- 15
- Pages
- 2342-2344
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W2161907482
- Cited by
- 46
- References
- 36
- Citations by year
- 2025: 2 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2017: 1 · 2016: 2 · 2015: 1 · 2014: 2 · 2013: 2 · 2012: 6
- FWCI
- 1.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The FASEB Journal (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (SFB 503, Project A3, Project B1)
- Topics
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Keywords
- nitrite, UVA irradiation, apoptosis, endothelial cells, lipid peroxidation, nitric oxide, photoprotection, cPTIO, butylated hydroxytoluene, nitrate
- MeSH terms
- Cells, Cultured, Cyclic N-Oxides, Dose-Response Relationship, Drug, Electron Spin Resonance Spectroscopy, Endothelium, Humans, Imidazoles, Models, Biological, Nitric Oxide, Nitrites, Radiography, Ultraviolet Rays, Lipid Peroxidation, Free Radical Scavengers, Apoptosis, Cytoprotection
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