Sustained delivery of proangiogenic microRNA‐132 by nanoparticle transfection improves endothelial cell transplantation

Abstract

Transplantation of endothelial cells (ECs) for therapeutic vascularization or tissue engineering is a promising method for increasing tissue perfusion. Here, we report on a new approach for enhanced EC transplantation using targeted nanoparticle transfection to deliver proangiogenic microRNA‐132 (miR‐132) to cultured ECs before their transplantation, thereby sensitizing cells to the effects of endogenous growth factors. We synthesized biodegradable PLGA polymer nanoparticles (NPs) that were loaded with miR‐132 and coated with cyclic RGD (cRGD) peptides that target integrin αvβ3 expressed on cultured human umbilical vein ECs (HUVECs), increasing NP uptake through clathrin‐coated pits. Unlike previously reported NPs for miR delivery, these NPs slowly release RNA for several weeks. The endocytosed NPs remain in clathrin‐coated vesicles from which they mediate intracellular delivery of siRNA or miRNA. Transfection of HUVECs with miR‐132 enhances growth factor‐induced proliferation and migration in 2D culture, producing a 1.8‐ and 5‐fold increase, respectively. However, while the effects of conventional transfection were short‐lived, NP transfection produced protein knockdown and biological effects that were significantly longer in duration (≥6 d). Transfection of HUVECs with miR‐132 NP resulted in a 2‐fold increase in the number of microvessels per square millimeter compared to lipid after transplantation into immunodeficient mice and led to a higher number of mural cell‐invested vessels than control transfection. These data suggest that sustained delivery of miR‐132 encapsulated in a targeted biodegradable polymer NP is a safe and efficient strategy to improve EC transplantation and vascularization.—Devalliere, J., Chang, W. G., Andrejecsk, J. W., Abrahimi, P., Cheng, C. J., Jane‐wit, D., Saltzman, W. M., Pober, J. S. Sustained delivery of proangiogenic microRNA‐132 by nanoparticle transfection improves endothelial cell transplantation. FASEB J. 28, 908–922 (2014). www.fasebj.org