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Cited by 84Nitric Oxide and Endothelin EffectsCardiac Ischemia and ReperfusionAntioxidant Activity and Oxidative Stress

Inhaled Nitric Oxide Attenuates Reperfusion Inflammatory Responses in Humans

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Mathru, Mali, Huda, Ruksana, Solanki, Daneshvari R., Hays, Stephen, Lang, John D.·2007·Anesthesiology
DOI 10.1097/00000542-200702000-00015
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Abstract

Background: Reduced bioavailability of endothelium-derived nitric oxide associated with reperfusion could potentially exacerbate the inflammatory response during reperfusion. Evidence suggests the pharmacologic effects of inhaled nitric oxide may extend beyond the pulmonary vasculature, and this is attributed to nitric oxide-derived complexes in blood that ultimately orchestrate antiinflammatory effects. In this study, the authors evaluated the potential for inhaled nitric oxide (80 ppm) to attenuate inflammation instigated by ischemia-reperfusion in a human model using patients undergoing knee surgery where a tourniquet was used to produce a bloodless surgical field. Methods: Inhaled nitric oxide (80 ppm) was administered before tourniquet application and continued throughout reperfusion until the completion of surgery. Venous blood samples were collected before and after reperfusion, for the measurements of nitrate and nitrite, CD11b/CD18, soluble P-selectin, and lipid hydroperoxide. Muscle biopsies were obtained from the quadriceps muscle before skin closure and analyzed for myeloperoxide, conjugated dienes, and nuclear factor-kappaB translocation. Results: Administration of inhaled nitric oxide (80 ppm) significantly attenuated the inflammatory response characterized by reduced expression of CD11b/CD18, P-selectin, and nuclear factor kappaB compared with the control group. This was accompanied by increased plasma levels of nitrate and nitrite and reduced oxidative stress. Conclusions: Administration of inhaled nitric oxide at 80 ppm significantly reduces inflammation in lower extremity ischemia-reperfusion in humans. This observation supports the concept that during diseases characterized by dysfunction in nitric oxide metabolism, inhaled nitric oxide may be an effective therapy to replenish systemic nitric oxide, thus retarding inflammatory-mediated injury.

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Cited by

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Authors
Mathru, M., Huda, R., Solanki, D. R., Hays, S., & Lang, J. D.
Affiliations
  • University of Alabama at Birmingham (US)
  • Instructor of Anesthesiology.
  • Professor of Anesthesiology, Department of Anesthesiology.
  • The University of Texas Medical Branch at Galveston (US)
  • University of Washington (US)
ORCID
Venue
Anesthesiology
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2007-02-01
Volume
106
Issue
2
Pages
275-282
ISSN
0003-3022 (ISSN-L)
DOI
10.1097/00000542-200702000-00015
OpenAlex
W1980292532
Cited by
84
References
3
Citations by year
2025: 1 · 2024: 4 · 2023: 4 · 2022: 2 · 2021: 3 · 2020: 2 · 2019: 7 · 2018: 5 · 2017: 6 · 2016: 3 · 2015: 6 · 2014: 8 · 2013: 4 · 2012: 5
FWCI
4.23
Type
journal article
Language
en
Open access
Locations
  • Anesthesiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Cardiac Ischemia and Reperfusion
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
Keywords
inhaled nitric oxide, ischemia-reperfusion injury, inflammation, nitric oxide bioavailability, tourniquet, knee surgery, CD11b/CD18, P-selectin, nuclear factor-kappaB, oxidative stress, nitrite/nitrate, conjugated dienes, quadriceps, lower limb ischemia
MeSH terms
Administration, Inhalation, Adult, Female, Humans, Inflammation, Lipid Peroxides, Male, Peroxidase, Nitrates, Nitric Oxide, Reperfusion, Macrophage-1 Antigen, NF-kappa B, P-Selectin, Lower Extremity

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