Enhanced S -Nitroso-Albumin Formation From Inhaled NO During Ischemia/Reperfusion
Abstract
In the present study, we investigated whether inhaled nitric oxide (NO) was transported by plasma proteins, such as S-nitroso-albumin (SNO-Alb), in the feline circulation and whether this molecule delivers NO to the periphery under conditions of stress, specifically ischemia/reperfusion (I/R). A flow probe was interposed between the femoral and superior mesenteric artery for blood flow measurements, and a branch of the superior mesenteric vein was cannulated for arterial-venous sampling. In animals breathing room air, SNO-Alb was below detection level in arterial or venous blood. NO inhalation resulted in a significant arterial-venous gradient for SNO-Alb. Concomitant with this loss of SNO-Alb across the intestinal vasculature was an increase in nitrite (NO2-). However, this release of NO was not sufficient to alter intestinal blood flow. I/R during NO inhalation caused a very large increase in arterial SNO-Alb that permitted a 5-fold increase in SNO-Alb consumption and significant generation of NO2- within the postischemic intestinal vasculature. The increased SNO-Alb consumption was sufficient to dramatically improve intestinal blood flow. The very large burst of arterial SNO-Alb during I/R was completely blocked by the administration of superoxide dismutase, suggesting that oxidative stress contributed to the increased SNO-Alb formation. Our data suggest that inhaled NO can increase nitrosothiol production and these molecules may be a functional NO delivery system during cardiovascular disease.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Nitrite reduction back to NO under appropriate pathophysiological conditions can generate many nitroso species, or nitrite itself may mediate intracellular nitrosation reactions such as RSNO formation.
“Recent studies show the potential role of nitrite reduction back to NO under appropriate pathophysiological conditions to generate many nitroso species or that nitrite itself may mediate intracellular nitrosation reactions (e.g. RSNO formation) [22, 23].”
From Inorganic nitrite therapy: historical perspective and future directions (2011) · cites this paper as [23]
Cites
Stamler, J. S., Simon, D. I., Osborne, J. A., Mullins, M. E., Jaraki, O., Michel, T. M., Singel, D. J., & Loscalzo, J. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.1.444 · Cited by 1,471 · see citing works
Nitric oxide circulates in mammalian plasma primarily as an S-nitroso adduct of serum albumin.
Stamler, J. S., Jaraki, O., Osborne, J., Simon, D. I., Keaney, J., Vita, J., Singel, D., Valeri, C. R., & Loscalzo, J. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.16.7674 · Cited by 1,238 · see citing works
Methods in nitric oxide research
Feelisch, M., & Stamler, J. S. (1996) · Wiley-Blackwell eBooks · Cited by 819 · see citing works
Xanthine oxidase and neutrophil infiltration in intestinal ischemia
Grisham, M. B., Hernandez, L. A., & Granger, D. N. (1986) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1986.251.4.g567 · Cited by 722 · see citing works
Lauer, T., Preik, M., Rassaf, T., Strauer, B. E., Deussen, A., Feelisch, M., & Kelm, M. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.221381098 · Cited by 585 · see citing works
The oxyhemoglobin reaction of nitric oxide
Gow, A. J., Luchsinger, B. P., Pawloski, J. R., Singel, D. J., & Stamler, J. S. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.16.9027 · Cited by 414 · see citing works
Feelisch, M., Rassaf, T., Mnaimneh, S., Singh, N., Bryan, N. S., Jourd'Heuil, D., & Kelm, M. (2002) · The FASEB Journal · doi:10.1096/fj.02-0363com · Cited by 401 · see citing works
Kinetics of Nitrosation of Thiols by Nitric Oxide in the Presence of Oxygen
Kharitonov, V. G., Sundquist, A. R., & Sharma, V. S. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.47.28158 · Cited by 365 · see citing works
NO forms an adduct with serum albumin that has endothelium-derived relaxing factor-like properties.
Keaney, J. F., Simon, D. I., Stamler, J. S., Jaraki, O., Scharfstein, J., Vita, J. A., & Loscalzo, J. (1993) · Journal of Clinical Investigation · doi:10.1172/jci116364 · Cited by 298 · see citing works
Cannon, R. O., Schechter, A. N., Panza, J. A., Ognibene, F. P., Pease-Fye, M. E., Waclawiw, M. A., Shelhamer, J. H., & Gladwin, M. T. (2001) · Journal of Clinical Investigation · doi:10.1172/jci200112761 · Cited by 298 · see citing works
Pharmacology of the Endothelium in Ischemia-Reperfusion and Circulatory Shock
Lefer, A. M., & Lefer, D. J. (1993) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pa.33.040193.000443 · Cited by 292 · see citing works
Cannon, R. O., Schechter, A. N., Panza, J. A., Ognibene, F. P., Pease‐Fye, M. E., Waclawiw, M. A., Shelhamer, J. H., & Gladwin, M. T. (2001) · Journal of Clinical Investigation · doi:10.1172/jci12761 · Cited by 289 · see citing works
Gladwin, M. T., Ognibene, F. P., Pannell, L. K., Nichols, J. S., Pease‐Fye, M. E., Shelhamer, J. H., & Schechter, A. N. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.180155397 · Cited by 274 · see citing works
Fox-Robichaud, A., Payne, D., Hasan, S. U., Ostrovsky, L., Fairhead, T., Reinhardt, P., & Kubes, P. (1998) · Journal of Clinical Investigation · doi:10.1172/jci2736 · Cited by 227 · see citing works
Evidence for in vivo transport of bioactive nitric oxide in human plasma
Rassaf, T., Preik, M., Kleinbongard, P., Lauer, T., Heiß, C., Strauer, B. E., Feelisch, M., & Kelm, M. (2002) · Journal of Clinical Investigation · doi:10.1172/jci14995 · Cited by 224 · see citing works
Chemical nature of nitric oxide storage forms in rat vascular tissue
Rodriguez, J., Maloney, R. E., Rassaf, T., Bryan, N. S., & Feelisch, M. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0234600100 · Cited by 210 · see citing works
Oxidation and Nitrosation of Thiols at Low Micromolar Exposure to Nitric Oxide
Jourd’heuil, D., Jourd’heuil, F. L., & Feelisch, M. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m300203200 · Cited by 194 · see citing works
Delivery and monitoring of inhaled nitric oxide in patients with pulmonary hypertension
WESSEL, D. L., Adatia, I., Thompson, J. E., & Hickey, P. R. (1994) · Critical Care Medicine · doi:10.1097/00003246-199406000-00009 · Cited by 188 · see citing works
Evidence for in vivo transport of bioactive nitric oxide in human plasma
Rassaf, T., Preik, M., Kleinbongard, P., Lauer, T., Heiß, C., Strauer, B. E., Feelisch, M., & Kelm, M. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0214995 · Cited by 183 · see citing works
Rassaf, T., Kleinbongard, P., Preik, M., Dejam, A., Gharini, P., Lauer, T., Erckenbrecht, J., Duschin, A., Schulz, R., Heusch, G., et al. (2002) · Circulation Research · doi:10.1161/01.res.0000035038.41739.cb · Cited by 178 · see citing works
van der Vliet, A., Hoen, P. A. C. ’., Wong, P., Bast, A., & Cross, C. E. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.46.30255 · Cited by 150 · see citing works
Protective role of NO in hepatic microcirculatory dysfunction during endotoxemia
Nishida, J., McCuskey, R. S., McDonnell, D., & Fox, E. S. (1994) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1994.267.6.g1135 · Cited by 138 · see citing works
Elevated Levels of <i>S</i> -Nitrosoalbumin in Preeclampsia Plasma
Tyurin, V. A., Liu, S.-X., Tyurina, Y. Y., Sussman, N. B., Hubel, C. A., Roberts, J. M., Taylor, R. N., & Kagan, V. E. (2001) · Circulation Research · doi:10.1161/hh1101.092179 · Cited by 120 · see citing works
Gut Ischemia Mediates Lung Injury by a Xanthine Oxidase-Dependent Neutrophil Mechanism
Koike, K., Moore, F. A., Moore, E. E., Read, R. A., Carl, V. S., & Banerjee, A. N. (1993) · Journal of Surgical Research · doi:10.1006/jsre.1993.1072 · Cited by 91 · see citing works
Prevention of ischemia-reperfusion lung injury by inhaled nitric oxide in neonatal piglets
Barbotin-Larrieu, F., Mazmanian, M. G., Baudet, B., Détruit, H., Chapelier, A. R., Libert, J., Dartevelle, P., & Hervé, P. (1996) · Journal of Applied Physiology · doi:10.1152/jappl.1996.80.3.782 · Cited by 86 · see citing works
Extra-pulmonary effects of inhaled nitric oxide in swine with and without phenylephrine
Troncy, É., Francoeur, M., Salazkin, I., Yang, F., Charbonneau, M., Leclerc, G., Vinay, P., & Blaise, G. A. (1997) · British Journal of Anaesthesia · doi:10.1093/bja/79.5.631 · Cited by 76 · see citing works
Kermarrec, N., Zunic, P., Béloucif, S., Bénessiano, J., Drouet, L. O., & Payen, D. M. (1998) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.158.3.9709097 · Cited by 63 · see citing works
Nitrosothiol Quantification in Human Plasma
Goldman, R. K., Vlessis, A. A., & Trunkey, D. D. (1998) · Analytical Biochemistry · doi:10.1006/abio.1998.2651 · Cited by 61 · see citing works
Synthesis and anti-inflammatory activity of a chimeric recombinant superoxide dismutase: SOD2/3
Gao, B., Flores, S. C., Leff, J. A., Bose, S. K., & McCord, J. M. (2003) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00374.2002 · Cited by 58 · see citing works
Nitrosyl hemoglobin in blood of normoxic and hypoxic sheep during nitric oxide inhalation
Takahashi, Y. K., Kobayashi, H., Tanaka, N., Sato, T., Takizawa, N., & Tomita, T. (1998) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.1998.274.1.h349 · Cited by 50 · see citing works
Effect of Thiol Status on Nitric Oxide Metabolism in the Circulation
Minamiyama, Y., Takemura, S., & Inoue, M. (1997) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.1997.9956 · Cited by 49 · see citing works
Inhaled NO impacts vascular but not extravascular compartments in postischemic peripheral organs
Kubes, P., Payne, D., Grisham, M. B., Jourd’heuil, D., & Fox‐Robichaud, A. (1999) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.1999.277.2.h676 · Cited by 47 · see citing works
Inhaled nitric oxide at the time of harvest improves early lung allograft function
Fujino, S., Nagahiro, I., Triantafillou, A. N., Boasquevisque, C. H. R., Yano, M., Cooper, J. D., & Patterson, G. A. (1997) · The Annals of Thoracic Surgery · doi:10.1016/s0003-4975(97)00236-1 · Cited by 41 · see citing works
Ma, X. L., Gao, F., Lopez, B. L., Christopher, T. A., & Vinten‐Johansen, J. (2000) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(24)35370-4 · Cited by 41 · see citing works
The pharmacology of inhaled nitric oxide.
Edwards, A. D. (1995) · Archives of Disease in Childhood Fetal & Neonatal · doi:10.1136/fn.72.2.f127 · Cited by 35 · see citing works
Malmros, C., Blomquist, S., Dahm, P., Mårtensson, L., & Thörne, J. (1996) · Critical Care Medicine · doi:10.1097/00003246-199605000-00019 · Cited by 33 · see citing works
Kuo, W., & Kocis, J. M. (2002) · Molecular and Cellular Biochemistry · doi:10.1023/a:1015510207489 · Cited by 27 · see citing works
Brigham, K. L. (1994) · CRC Press eBooks · doi:10.1201/9781439805510 · Cited by 23 · see citing works
Inhaled Nitric Oxide in Anesthesia and Critical Care Medicine
Kavanagh, B. P., & Pearl, R. G. (1995) · International Anesthesiology Clinics · doi:10.1097/00004311-199500000-00010 · Cited by 19 · see citing works
Inhaled Nitric Oxide in Anesthesia and Critical Care Medicine
Kavanagh, B. P., & Pearl, R. G. (1994) · International Anesthesiology Clinics · doi:10.1097/00004311-199432010-00012 · Cited by 15 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Oettl, K., & Stauber, R. E. (2007) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0707251 · Cited by 359 · see citing works
“…is in the nanomolar range (Marley et al., 2001; Tsikas and Frölich, 2002; Tsikas et al., 2002; Ng et al., 2004; Stamler, 2004; Zhang and Hogg, 2005), while under pathological conditions, micromolar concentrations of SNO-alb may occur (Ottesen et al., 2001; Bayir et al., 2003; Ng et al., 2004).”
BackgroundDejam, A., Hunter, C. J., Tremonti, C., Pluta, R. M., Hon, Y. Y., Grimes, G., Partovi, K., Pelletier, M. M., Oldfield, E. H., Cannon, R. O., et al. (2007) · Circulation · doi:10.1161/circulationaha.107.712133 · Cited by 334 · see citing works
Gladwin, M. T., Raat, N. J. H., Shiva, S., Dezfulian, C., Hogg, N., Kim-Shapiro, D. B., & Patel, R. P. (2006) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00407.2006 · Cited by 329 · see citing works
“Studies by Ng and colleagues (68), evaluating the effects of 80 ppm NO gas inhalation on feline intestinal ischemia-reperfusion blood flow, revealed increases in plasma nitrite from 100 to 489 nM (68), well above the effective doses of nitrite observed in the Duranski studies.”
BackgroundInfluentialDirect evidence for S-nitrosation of mitochondrial complex I
Burwell, L. S., Nadtochiy, S. M., Tompkins, A. J., Young, S., & Brookes, P. S. (2006) · Biochemical Journal · doi:10.1042/bj20051435 · Cited by 274 · see citing works
S -Nitrosylation: NO-Related Redox Signaling to Protect Against Oxidative Stress
Sun, J., Steenbergen, C., & Murphy, E. (2006) · Antioxidants and Redox Signaling · doi:10.1089/ars.2006.8.1693 · Cited by 235 · see citing works
Lang, J. D., Teng, X., Chumley, P., Crawford, J. H., Isbell, T. S., Chacko, B. K., Liu, Y., Jhala, N., Crowe, D. R., Smith, A. B., et al. (2007) · Journal of Clinical Investigation · doi:10.1172/jci31892 · Cited by 224 · see citing works
Gonzalez, F. M., Shiva, S., Vincent, P. S., Ringwood, L. A., Hsu, L.-Y., Hon, Y. Y., Aletras, A. H., Cannon, R. O., Gladwin, M. T., & Arai, A. E. (2008) · Circulation · doi:10.1161/circulationaha.107.748814 · Cited by 175 · see citing works
<i>S</i> -Nitrosothiols in the Blood: Roles, Amounts, and Methods of Analysis
Stamler, J. S. (2004) · Circulation Research · doi:10.1161/01.res.0000122071.55721.bc · Cited by 170 · see citing works
Hemoglobin, nitric oxide and molecular mechanisms of hypoxic vasodilation
Allen, B. W., Stamler, J. S., & Piantadosi, C. A. (2009) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2009.08.002 · Cited by 165 · see citing works
Hataishi, R., Rodrigues, A. C. T., Neilan, T. G., Morgan, J. G., Buys, E. S., Shiva, S., Tambouret, R., Jassal, D. S., Raher, M. J., Furutani, E., et al. (2006) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.01172.2005 · Cited by 159 · see citing works
“NO may be carried by the blood to the reperfused myocardium by S-nitrosothiols (35, 44), Snitrosohemoglobin (45), nitrosylhemoglobin (48), and nitrite (9).”
BackgroundInhaled NO as a therapeutic agent
Bloch, K. D., Ichinose, F., ROBERTSJR, J., & Zapol, W. M. (2007) · Cardiovascular Research · doi:10.1016/j.cardiores.2007.04.014 · Cited by 159 · see citing works
“Cannon and colleagues did not detect an increase in plasma S-nitrosothiols or SNO-Hb after breathing NO for 1 h. In contrast, Ng and colleagues reported that, in cats, breathing 80 ppm NO increased plasma levels of Snitrosoalbumin and nitrite, both of which were further increased in the setting of intestinal ischemia…”
BackgroundHeterogeneity and oxidation status of commercial human albumin preparations in clinical use*
Bar‐Or, D., Bar-Or, R., Rael, L. T., Gardner, D. K., Slone, D. S., & Craun, M. L. (2005) · Critical Care Medicine · doi:10.1097/01.ccm.0000169876.14858.91 · Cited by 157 · see citing works
Nadtochiy, S. M., Burwell, L. S., & Brookes, P. S. (2007) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2007.01.010 · Cited by 142 · see citing works
Tsikas, D. (2008) · Analytical Biochemistry · doi:10.1016/j.ab.2008.04.018 · Cited by 131 · see citing works
Giustarini, D., Milzani, A., Dalle‐Donne, I., & Rossi, R. (2006) · Journal of Chromatography B · doi:10.1016/j.jchromb.2006.09.031 · Cited by 123 · see citing works
Liu, X., Huang, Y., Pokreisz, P., Vermeersch, P., Marsboom, G., Swinnen, M., Verbeken, E. K., Santos, J., Pellens, M., Gillijns, H., et al. (2007) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2007.04.069 · Cited by 118 · see citing works
Inorganic nitrite therapy: historical perspective and future directions
Kevil, C. G., Kolluru, G. K., Pattillo, C. B., & Giordano, T. (2011) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2011.04.042 · Cited by 113 · see citing works
S-Nitrosylation signaling regulates cellular protein interactions
Marozkina, N. V., & Gaston, B. M. (2011) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2011.06.017 · Cited by 98 · see citing works
Abu‐Amara, M., Yang, S. Y., Seifalian, A. M., Davidson, B. R., & Fuller, B. J. (2012) · Liver International · doi:10.1111/j.1478-3231.2012.02755.x · Cited by 92 · see citing works
How do red blood cells cause hypoxic vasodilation? The SNO-hemoglobin paradigm
Allen, B. W., & Piantadosi, C. A. (2006) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00310.2006 · Cited by 90 · see citing works
“However, this inference disregards the more straightforward explanation that such a gradient reflects the oxidation of EDRF to nitrite as blood transits the microcirculatory unit from artery to vein (31).”
BackgroundGladwin, M. T., & Schechter, A. N. (2004) · Circulation Research · doi:10.1161/01.res.0000126697.64381.37 · Cited by 85 · see citing works
McMahon, T. J., & Doctor, A. (2006) · Proceedings of the American Thoracic Society · doi:10.1513/pats.200507-066bg · Cited by 87 · see citing works
Myocardial protection by nitrite
Calvert, J. W., & Lefer, D. J. (2009) · Cardiovascular Research · doi:10.1093/cvr/cvp079 · Cited by 82 · see citing works
Ruan, S., Huang, T., Wu, H., Wu, H., Yu, C., & Lai, M. (2015) · Critical Care · doi:10.1186/s13054-015-0880-2 · Cited by 77 · see citing works
“Cumulative data from physiological studies and clinical trials suggest that iNO therapy has a good safety profile [8-10], and that its potential adverse effects, including methemoglobinemia, inhibition of platelet aggregation and systemic vasodilatation, are usually clinically insignificant [8,11,12].”
BackgroundWorking with nitric oxide and hydrogen sulfide in biological systems
Yuan, S. S., Patel, R. P., & Kevil, C. G. (2014) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00327.2014 · Cited by 74 · see citing works
Bice, J. S., Jones, B. R., Chamberlain, G. R., & Baxter, G. F. (2016) · Basic Research in Cardiology · doi:10.1007/s00395-016-0540-y · Cited by 72 · see citing works
Clinical translation of nitrite therapy for cardiovascular diseases
Calvert, J. W., & Lefer, D. J. (2010) · Nitric Oxide · doi:10.1016/j.niox.2009.11.001 · Cited by 72 · see citing works
Inhaled Nitric Oxide Attenuates Reperfusion Inflammatory Responses in Humans
Mathru, M., Huda, R., Solanki, D. R., Hays, S., & Lang, J. D. (2007) · Anesthesiology · doi:10.1097/00000542-200702000-00015 · Cited by 84 · see citing works
“cept that replenishing nitric oxide may provide an efficacious therapy in ischemia–reperfusion injury.(10) In this”
BackgroundResultNitric oxide and blood: a review
Wallis, J. P. (2005) · Transfusion Medicine · doi:10.1111/j.1365-3148.2005.00542.x · Cited by 67 · see citing works
“In support of this, Ng et al. (2004) have recently shown that in cats breathing NO, arterial AlbuminSNO increased up to 300 nmol L 1 and was further increased in the presence of ischaemic injury.”
BackgroundTrzeciak, S., Glaspey, L. J., Dellinger, R. P., Durflinger, P., Anderson, K., Dezfulian, C., Roberts, B. W., Chansky, M. E., Parrillo, J. E., & Hollenberg, S. M. (2014) · Critical Care Medicine · doi:10.1097/ccm.0000000000000549 · Cited by 60 · see citing works
“Age, yr, mean (sd) 59 (17) 59 (15) 58 (20)”
Role of the Red Blood Cell in Nitric Oxide Homeostasis and Hypoxic Vasodilation
Gladwin, M. T. (2007) · Advances in Experimental Medicine and Biology · doi:10.1007/978-0-387-34817-9_17 · Cited by 59 · see citing works
“... WKLV�oHOG�LV�H[WUHPHO\�FRQWURYH UVLDO��ODUJHO\�VHFRQGDU\� to major questions about the concentrations and importance of SNO-albumin and SNO-he-PRJORELQ�LQ�WKH�KXPDQ�FLUFXODWLRQ��UHSRUWHG�YDOXHV�UDQJH�IURP�D with more modern methodologies documenting levels of less than 10 nM) (18, 20, 50, 70, 71, 84, 92), it is li…”
BackgroundMethodInhaled nitric oxide: Current clinical concepts
Bhatraju, P. K., Crawford, J. H., Hall, M. K., & Lang, J. D. (2015) · Nitric Oxide · doi:10.1016/j.niox.2015.08.007 · Cited by 57 · see citing works
Inactivation of Soluble Guanylate Cyclase by Stoichiometric S-Nitrosation
Mayer, B., Kleschyov, A. L., Stessel, H., Russwurm, M., Münzel, T. F., Koesling, D., & Schmidt, K. (2008) · Molecular Pharmacology · doi:10.1124/mol.108.052142 · Cited by 55 · see citing works
“lished previously (Ng et al., 2004).”
Nitric oxide signaling in the brain: A new target for inhaled nitric oxide?
Charriaut‐Marlangue, C., Bonnin, P., Pham, H., Loron, G., Léger, P., Gressèns, P., Renolleau, S., & Baud, O. (2013) · Annals of Neurology · doi:10.1002/ana.23842 · Cited by 50 · see citing works
Nitric Oxide and the Cardiovascular System
Bohlen, H. G. (2015) · Comprehensive physiology · doi:10.1002/cphy.c140052 · Cited by 54 · see citing works
Inhaled pulmonary vasodilators: a narrative review
Liu, K., Wang, H., Yu, S.-J., Tu, G.-W., & Luo, Z. (2021) · Annals of Translational Medicine · doi:10.21037/atm-20-4895 · Cited by 47 · see citing works
“Biochemistry and physiological actions NO will react with oxygen, other free radical species, various amino acids, and transition metals, with a potential for nitrogen dioxide (NO2), peroxynitrite, and methemoglobin (7,8).”
BackgroundRole of Circulating S -Nitrosothiols in Control of Blood Pressure
Foster, M. W., Pawloski, J. R., Singel, D. J., & Stamler, J. S. (2004) · Hypertension · doi:10.1161/01.hyp.0000150160.41992.71 · Cited by 44 · see citing works
Derwall, M., Ebeling, A., Nolte, K. W., Weis, J., Rossaint, R., Ichinose, F., Nix, C., Fries, M., & Brücken, A. (2015) · Critical Care · doi:10.1186/s13054-015-1050-2 · Cited by 43 · see citing works
“The ability of iNO to reduce I/R (ischemia/reperfusion) injury was subsequently reproduced in proof-of-principle human studies [7, 27].”
MethodLang, J. D., Smith, A. B., Brandon, A. P., Bradley, K. M., Liu, Y., Li, W., Crowe, D. R., Jhala, N. C., Cross, R. C., Frenette, L., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0086053 · Cited by 40 · see citing works
“S-nitrosoalbumin), whereas studies in humans and mice indicate nitrite as a possible mediator [35].”
BackgroundBrücken, A., Derwall, M., Bleilevens, C., Stoppe, C., Goetzenich, A., Gaisa, N. T., Weis, J., Nolte, K. W., Rossaint, R., Ichinose, F., et al. (2015) · Critical Care · doi:10.1186/s13054-015-1128-x · Cited by 40 · see citing works
“It has been suggested that NO is transported to remote organs as more stable bioactive molecules including iron nitrosyl [48], S-nitrosothiols [49], nitrite [50], and nitrosolipids [51].”
BackgroundHendgen-Cotta, U. B., Grau, M., Rassaf, T., Gharini, P. P. R., Kelm, M., & Kleinbongard, P. (2008) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(08)01216-0 · Cited by 39 · see citing works
Anti-inflammatory properties of a chimeric recombinant superoxide dismutase: SOD2/3
Hernández-Saavedra, D., Zhou, H., & McCord, J. M. (2005) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2005.03.001 · Cited by 38 · see citing works
Gladwin, M. T., Wang, X., & Hogg, N. (2006) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2006.05.025 · Cited by 35 · see citing works
Zhu, C., Sun, Y., Gao, J., Wang, X., Plesnila, N., & Blomgren, K. (2012) · Translational Stroke Research · doi:10.1007/s12975-012-0217-2 · Cited by 34 · see citing works
Franceschi, L. D., Malpeli, G., Scarpa, A., Janin, A., Muchitsch, E. M., Roncada, P., Lebœuf, C., Corrocher, R., Beuzard, Y., & Brugnara, C. (2006) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00462.2005 · Cited by 33 · see citing works
Bohlen, H. G., Zhou, X., Unthank, J. L., Miller, S. J., & Bills, R. G. (2009) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00171.2009 · Cited by 30 · see citing works
Guikema, B. J., Lu, Q., & Jourd’heuil, D. (2005) · Antioxidants and Redox Signaling · doi:10.1089/ars.2005.7.593 · Cited by 29 · see citing works
Angelis, D., Savani, R. C., & Chalak, L. F. (2020) · Pediatric Research · doi:10.1038/s41390-020-1017-0 · Cited by 29 · see citing works
Nitrosothiol stores in vascular tissue: Modulation by ultraviolet light, acetylcholine and ionomycin
Ng, E. S. M., Cheng, Z. A., Ellis, A., Ding, H., Jiang, Y., Li, Y., Hollenberg, M. D., & Triggle, C. R. (2007) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2007.01.016 · Cited by 29 · see citing works
“Furthermore, changes in the levels of nitrite, the major oxidative breakdown product of nitric oxide in biological fluids in the absence of hemoglobin (Lauer et al., 2002; Ng et al., 2004), served to indicate that nitric oxide was released from tissues and cells in our experimental protocols.”
BackgroundMethodInfluentialZhang, L., Croix, C. M. S., Cao, R., Wasserloos, K., Watkins, S. C., Stevens, T., Li, S., Tyurin, V. A., Kagan, V. E., & Pitt, B. R. (2006) · Experimental Biology and Medicine · doi:10.1177/153537020623100909 · Cited by 29 · see citing works
Emerging role of nitrite in myocardial protection
Lefer, D. J. (2009) · Archives of Pharmacal Research · doi:10.1007/s12272-009-1804-y · Cited by 27 · see citing works
“, 1998), 4) increase intestinal blood flow during concurrent NO synthase inhibition or after intestinal ischemia-reperfusion injury in cats (Fox-Robichaud et al., 1998; Kubes et al., 1999; Ng et al., 2004), 5) prevent forearm vasoconstriction during regional NO synthase inhibition in human volunteers (Rassaf et al.”
BackgroundMaher, A. R., Arif, S., Madhani, M., Abozguia, K., Ahmed, I., Fernandez, B. O., Feelisch, M., O'Sullivan, A., Christopoulos, A., Sverdlov, A. L., et al. (2013) · British Journal of Pharmacology · doi:10.1111/bph.12152 · Cited by 26 · see citing works
“…being indicative of NO generation and therefore indicative of diminished eNOS activity in CHF (Lauer et al., 2001), whereas RXNO concentrations reflect the generation of S-nitrosoproteins from either NO or NO2- (Bryan et al., 2005), as well as a measure of prevalent redox stress (Ng et al., 2004).”
BackgroundRoutes for Formation of S-Nitrosothiols in Blood
Nagababu, E., & Rifkind, J. M. (2011) · Cell Biochemistry and Biophysics · doi:10.1007/s12013-011-9321-2 · Cited by 25 · see citing works
“In spite of these kinetic constraints, reports indicate the formation of RSNO in plasma following the incubation of NO donors with whole blood [42], intravenous infusion of NO in human subjects [44], inhalation of NO [43, 98], and”
BackgroundWang, Q., Akhavan, B., Jing, F., Cheng, D., Sun, H. L., Xie, D., Leng, Y. X., Bilek, M., & Huang, N. (2018) · Advanced Materials Interfaces · doi:10.1002/admi.201701487 · Cited by 24 · see citing works
Baldim, V., Ismail, A., Taladriz‐Blanco, P., Griveau, S., de Oliveira, M. G., & Fethi Bedioui, A. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.5b04035 · Cited by 20 · see citing works
Nitric oxide facilitates the targeting Kupffer cells of a nano-antioxidant for the treatment of NASH
Maeda, H., Ishima, Y., Saruwatari, J., Mizuta, Y., Minayoshi, Y., Ichimizu, S., Yanagisawa, H., Nagasaki, T., Yasuda, K., Oshiro, S., et al. (2021) · Journal of Controlled Release · doi:10.1016/j.jconrel.2021.11.039 · Cited by 19 · see citing works
Can Nitric Oxide–Based Therapy Prevent Bronchopulmonary Dysplasia?
Raffay, T. M., Martin, R. J., & Reynolds, J. D. H. (2012) · Clinics in Perinatology · doi:10.1016/j.clp.2012.06.011 · Cited by 18 · see citing works
Yi, J., Khan, M. A., Lee, J., & Richter-Addo, G. B. (2005) · Nitric Oxide · doi:10.1016/j.niox.2005.03.005 · Cited by 18 · see citing works
Bonder, C. S., Knight, D., Hernández-Saavedra, D., McCord, J. M., & Kubes, P. (2004) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00049.2004 · Cited by 17 · see citing works
“This dose of 1 U/g body wt was chosen on the basis of previous reports that these protocols were efficacious in vivo in other inflammatory models (5, 9, 16, 39).”
MethodInhaled nitric oxide does not reduce systemic vascular resistance in mice
Hataishi, R., Zapol, W. M., Bloch, K. D., & Ichinose, F. (2005) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00938.2005 · Cited by 16 · see citing works
“While our current results do not suggest that enough NO or NO adducts reach the periphery to cause vasodilation, these findings are not surprising in light of recent reports by Ng and colleagues (11), who found that mesenteric I/R, but not NOS inhibition by L-NAME, was associated with sufficient production and consump…”
ResultBohlen, H. G. (2012) · Microcirculation · doi:10.1111/micc.12003 · Cited by 15 · see citing works
“Measurements of S-nitrosothiol compounds have used light and chemical measurement assays rather than NO-sensitive electrodes [18,25,28,29,31,32].”
BackgroundMethodSummary: Systemic Effects of Inhaled Nitric Oxide
Gaston, B. M. (2006) · Proceedings of the American Thoracic Society · doi:10.1513/pats.200506-049bg · Cited by 15 · see citing works
“More recently, Ng and coworkers ( 9 ) have gone back to this model of the effect of inhaled NO on mesenteric ischemia to investigate the mechanism by which the systemic effect is caused.”
BackgroundS -Nitrosoalbumin and Other S -Nitrosothiols in the Blood: Is Their Quantity of No Relevance?
Tsikas, D. (2004) · Circulation Research · doi:10.1161/res.94.12.e106 · Cited by 14 · see citing works
Goździk, W., Zieliński, S., Zielińska, M., Ratajczak, K., Skrzypczak, P., Rodziewicz, S., Kübler, A., Löfström, K., Dzięgiel, P., Olbromski, M., et al. (2018) · International Journal of Immunopathology and Pharmacology · doi:10.1177/0394632017751486 · Cited by 13 · see citing works
Hess, D. T., Foster, M. W., & Stamler, J. S. (2009) · Circulation · doi:10.1161/circulationaha.109.876607 · Cited by 13 · see citing works
Nitric Oxide and the Cardiovascular System
Bohlen, H. G. (2015) · Comprehensive physiology · doi:10.1002/j.2040-4603.2015.tb00628.x · Cited by 12 · see citing works
Sustained lung activity of a novel chimeric protein, SOD2/3, after intratracheal administration
Clarke, M. B., Wright, R., Irwin, D. C., Bose, S. K., Rheen, Z. V., Birari, R. B., Stenmark, K. R., McCord, J. M., & Nozik‐Grayck, E. (2010) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2010.09.028 · Cited by 12 · see citing works
Stojanović, I. R., Djordjević, G., Pavlović, R., Djordjević, V. B., Pavlovic, D. D., Cvetković, T., Ljubisavljević, S., Bašić, J., & Zabar, K. (2012) · Journal of the Neurological Sciences · doi:10.1016/j.jns.2012.09.026 · Cited by 11 · see citing works
“In addition, reactive oxygen species, have been found to potentiate albumin S-nitrosylation [35].”
BackgroundNitric oxide and thiols: Chemical biology, signalling paradigms and vascular therapeutic potential
Dent, M. R., & DeMartino, A. W. (2023) · British Journal of Pharmacology · doi:10.1111/bph.16274 · Cited by 10 · see citing works
“S -Nitrosated species (both proteins and/or small molecules) have been proposed to contribute to this systemic signalling during iNO treatment (McMahon & Doctor, 2006; Ng et al., 2004).”
Conahey, G. R., Power, G. G., Hopper, A. O., Terry, M. H., Kirby, L. S., & Blood, A. B. (2008) · Pediatric Research · doi:10.1203/pdr.0b013e318180f08b · Cited by 9 · see citing works
“Other possibilities include s-nitrosated proteins (RSNOs) (28,29), and iron-nitrosyl Hb, HbNO (5).”
BackgroundRole of nitric oxide in liver transplantation: Should it be routinely used?
Fukazawa, K., & Lang, J. D. (2016) · World Journal of Hepatology · doi:10.4254/wjh.v8.i34.1489 · Cited by 9 · see citing works
Bohlen, H. G. (2011) · Microcirculation · doi:10.1111/j.1549-8719.2011.00127.x · Cited by 9 · see citing works
Szabó, C. (2010) · Wiley eBooks · doi:10.1002/0471266949.bmc178 · Cited by 7 · see citing works
Endocrine Nitric Oxide Bioactivity and Hypoxic Vasodilation by Inhaled Nitric Oxide
Stamler, J. S., Reynolds, J. D. H., & Hess, D. T. (2012) · Circulation Research · doi:10.1161/circresaha.111.263996 · Cited by 7 · see citing works
Interaction between albumin-bound dinitrosyl iron complexes and reactive oxygen species
Шумаев, К. Б., Gubkin, A. A., Gubkina, S. A., Gudkov, L. L., Lakomkin, V. L., Topunov, A. F., Vanin, A. F., & Ruuge, E. K. (2007) · BIOPHYSICS · doi:10.1134/s0006350907030141 · Cited by 6 · see citing works
Troncy, É., Hubert, B., Pang, D., Taha, R. A., Gauvin, D., Beauchamp, G., Veldhuizen, R. A. W., & Blaise, G. A. (2006) · Nitric Oxide · doi:10.1016/j.niox.2006.01.004 · Cited by 6 · see citing works
dos Reis, R. A., Boudier, A., Piquard, F., Pieretti, J. C., Seabra, A. B., & Clarot, I. (2025) · Nitric Oxide · doi:10.1016/j.niox.2025.04.002 · Cited by 5 · see citing works
Inhaled nitric oxide and neonatal brain damage: experimental and clinical evidences
Sun, B. (2012) · The Journal of Maternal-Fetal & Neonatal Medicine · doi:10.3109/14767058.2012.665243 · Cited by 5 · see citing works
Matsushita, S., Nishi, K., Iwao, Y., Ishima, Y., Watanabe, H., Taguchi, K., Yamasaki, K., Maruyama, T., & Otagiri, M. (2017) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.b17-00528 · Cited by 4 · see citing works
Extrapulmonary Effects of Inhaled Nitric Oxide
McMahon, T., & Doctor, A. (2012) · Proceedings of the American Thoracic Society · Cited by 4 · see citing works
Ishibashi, T., Miwa, T., Nishizawa, N., Shinkawa, I., Yoshida, J., Kawada, T., & Nishio, M. (2011) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.34.1307 · Cited by 4 · see citing works
Inhaled Nitric Oxide—Current Practice and Future Potential Uses and Development
Ichinose, F., & Zapol, W. M. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-804273-1.00025-9 · Cited by 4 · see citing works
Ishibashi, T., Nishizawa, N., Nakamoto-Nomura, M., Abe, F., Liu, H., Yoshida, J., Kawada, T., & Nishio, M. (2011) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.34.528 · Cited by 3 · see citing works
PATHOPHYSIOLOGY OF HEMOGLOBIN AND ITS DISORDERS
Steinberg, M. H. (2009) · Cambridge University Press eBooks · doi:10.1017/cbo9780511596582.012 · Cited by 2 · see citing works
The Biology of Vascular Nitric Oxide
Leopold, J. A., & Loscalzo, J. (2009) · Cambridge University Press eBooks · doi:10.1017/cbo9780511596582.015 · Cited by 2 · see citing works
Inhaled Nitric Oxide Therapy for Extrapulmonary Inflammation
Crawford, J. H., Lang, J. D., & Patel, R. P. (2015) · Future Science OA · doi:10.4155/fso.15.34 · Cited by 1 · see citing works
Triggle, C. R., Ding, H., Ng, E. S. M., & Ellis, A. (2004) · Humana Press eBooks · doi:10.1007/978-1-59259-806-9_3 · Cited by 1 · see citing works
Perera, I. N., Sabbagh, T., Boutros, J. A., & Bayachou, M. (2009) · Talanta · doi:10.1016/j.talanta.2008.12.074 · Cited by 1 · see citing works
The Role of Reactive Oxygen and Nitrogen Species in Ischemia/Reperfusion Injury
Spagnolli, E., & Zapol, W. M. (2011) · Wiley eBooks · doi:10.1002/9781119975427.ch5 · Cited by 1 · see citing works
Sickle Cell Disease does not Decrease Pulmonary Nitric Oxide
Sundararajan, S., & Gaston, B. M. (2011) · The Journal of Pediatrics · doi:10.1016/j.jpeds.2011.08.024 · Cited by 1 · see citing works
(2007) · Advances in experimental biology · doi:10.1016/s1872-2423(07)01010-1
Adler, K. B., & Matalon, S. (2009) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/rcmb.2009-2007ed
Nitric Oxide and Inhaled Pulmonary Vasodilators
Ichinose, F., & Zapol, W. M. (2010) · Elsevier eBooks · doi:10.1016/b978-0-443-06959-8.00031-5
Fincher, S. H., & Butt, W. W. (2025) · Perfusion · doi:10.1177/02676591241239569
“Increased levels of these thiols have been shown with the use of inhaled NO. 153,154 These molecules will demonstrate vasoac-tivity in states of relative NO de fi ciency but are not dependent on hypoxia.”
The effect of inhaled nitric oxide on splanchnic perfusion in a neonatal piglet model
Hameedi, S. G., Wright, J. G., Hovell, M. E., Schafer, C. G., Kamr, A., Sbragia, L., & Olutoye, O. O. (2026) · Pediatric Research · doi:10.1038/s41390-026-04895-8
Ismail, A. G. (2016) · doi:10.70675/d94f53bbzc8bcz447bz898fz2047b4ad9fea · Cited by 1 · see citing works
“Methods of quantification of RSNOs The ideal method to quantify RSNOs is a method that i) has a LOD in the nanomolar range in order to detect LMW- RSNOs and in the micromolar range in order to HMW-RSNOs, ii) is selective to NO (no detection of nitrite or other biological molecule), iii) that does not underor over-esti…”
BackgroundInfluentialInvestigating the role of nitric oxide clones in cardiovascular conditions
Arif, S. (2014) · University of Birmingham Institutional Research Archive (University of Birmingham)
- Authors
- Ng, E. S. M., Jourd’heuil, D., McCord, J. M., Hernandez, D., Yasui, M., Knight, D., & Kubes, P.
- Affiliations
- University of Calgary (CA)
- From the Department of Physiology and Biophysics (E.S.M.N., D.K., P.K.), Immunology Research Group, University of Calgary, Calgary, Alberta, Canada; Center for Cardiovascular Sciences (D.J.), Albany Medical College, Albany, NY; and Webb-Waring Institute (J.M.M., D.H., M.Y.), University of Colorado Health Sciences Center, Denver, Colo.
- Venue
- Circulation Research
- Published
- 2004-03-05
- Volume
- 94
- Issue
- 4
- Pages
- 559-565
- ISSN
- 0009-7330 (ISSN-L)
- 1524-4571
- OpenAlex
- W2096856230
- Cited by
- 97
- References
- 40
- Citations by year
- 2026: 1 · 2025: 2 · 2023: 1 · 2021: 2 · 2020: 1 · 2018: 2 · 2017: 2 · 2016: 4 · 2015: 7 · 2014: 4 · 2013: 2 · 2012: 8
- FWCI
- 7.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Circulation Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Canada Research Chairs
- Canadian Institutes of Health Research
- National Cancer Institute (CA-89366, R01CA089366)
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hemoglobin structure and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- inhaled nitric oxide, ischemia-reperfusion, nitric oxide delivery, S-nitrosothiols, nitrite, intestinal blood flow, oxidative stress, superoxide dismutase, superior mesenteric artery, superior mesenteric vein, feline model
- MeSH terms
- Administration, Inhalation, Animals, Biological Transport, Cats, Constriction, Intestines, Ischemia, Nitric Oxide, Nitrites, Nitroso Compounds, Oxidation-Reduction, Recombinant Fusion Proteins, Serum Albumin, Bovine, Splanchnic Circulation, Superoxides, Superoxide Dismutase, Vasodilation, Reperfusion, Mesenteric Artery, Superior, Oxidative Stress, NG-Nitroarginine Methyl Ester, Nitric Oxide Donors, S-Nitrosoglutathione
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
