Cited by 73Alzheimer's disease research and treatmentsDementia and Cognitive Impairment ResearchNeuroinflammation and Neurodegeneration Mechanisms

Cells with a familial Alzheimerʼs disease mutation produce authentic β-peptide

Dovey, Harry F., Suomensaari-Chrysler, Susanna, Lieberburg, Ivan, Sinha, Sukanto, Keim, Pam S.·1993·NeuroReport

Abstract

Cells overexpressing the beta-amyloid precursor protein possessing a mutation found in familial Alzheimer's disease overproduce beta-amyloid peptide (A beta). Because these findings were based on immunological identification, we have chemically characterized the peptides produced. Purified A beta fragments from the conditioned media of these cells were found to have N-terminal sequence consistent with the A beta found in cerebral plaques. Mass spectrometric data demonstrated a series of A beta fragments consistent with those found in Alzheimer's disease (AD); the major species corresponding to A beta(1-40). Significantly, a longer fragment corresponding to A beta(1-42) was found. These findings suggest that this cellular system may be useful for mechanistic studies of A beta generation and possibly for the development of therapeutic agents to treat AD.

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Crossref · 10.1097/00001756-199308000-00011

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  • Aβs ending at Aβ42(43) are a minor component of the Aβ in human cerebrospinal fluid and plasma.

    “These observations indicate that Aps ending at Ap42(43), which are a minor component of the Ap in human cerebrospinal fluid and”

    From Amyloid β Protein (Aβ) in Alzheimeri's Disease Brain (1995) · cites this paper as [7]

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Authors
Dovey, H. F., Suomensaari-Chrysler, S., Lieberburg, I., Sinha, S., & Keim, P. S.
Venue
NeuroReport
Published
1993-08
Volume
4
Issue
8
Pages
1039-1042
ISSN
0959-4965 (ISSN-L)
OpenAlex
W44439419
Cited by
71
References
0
Citations by year
2023: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 1 · 2013: 2
FWCI
7.11
Type
journal article
Language
en
Open access
closed
Locations
  • Neuroreport (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
familial Alzheimer's disease, amyloid-beta, amyloid precursor protein, APP mutations, amyloid-beta 42, Aβ40, conditioned medium, peptide characterization, Alzheimer's disease
MeSH terms
Alzheimer Disease, Amino Acid Sequence, Cell Line, Chromatography, Enzyme-Linked Immunosorbent Assay, Humans, Immunologic Techniques, Molecular Sequence Data, Mutation, Mass Spectrometry, Amyloid beta-Peptides

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