Cells with a familial Alzheimerʼs disease mutation produce authentic β-peptide
Abstract
Cells overexpressing the beta-amyloid precursor protein possessing a mutation found in familial Alzheimer's disease overproduce beta-amyloid peptide (A beta). Because these findings were based on immunological identification, we have chemically characterized the peptides produced. Purified A beta fragments from the conditioned media of these cells were found to have N-terminal sequence consistent with the A beta found in cerebral plaques. Mass spectrometric data demonstrated a series of A beta fragments consistent with those found in Alzheimer's disease (AD); the major species corresponding to A beta(1-40). Significantly, a longer fragment corresponding to A beta(1-42) was found. These findings suggest that this cellular system may be useful for mechanistic studies of A beta generation and possibly for the development of therapeutic agents to treat AD.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Aβs ending at Aβ42(43) are a minor component of the Aβ in human cerebrospinal fluid and plasma.
“These observations indicate that Aps ending at Ap42(43), which are a minor component of the Ap in human cerebrospinal fluid and”
From Amyloid β Protein (Aβ) in Alzheimeri's Disease Brain (1995) · cites this paper as [7]
Cites
Still being checked.
Cited by
Sorted by citations
Scheuner, D., Eckman, C., Jensen, M., Song, X., Citron, M., Suzuki, N., Bird, T. D., Hardy, J., Hutton, M., Kukull, W., et al. (1996) · Nature Medicine · doi:10.1038/nm0896-864 · Cited by 2,599 · see citing works
Iwatsubo, T., Odaka, A., Suzuki, N., Mizusawa, H., Nukina, N., & Ihara, Y. (1994) · Neuron · doi:10.1016/0896-6273(94)90458-8 · Cited by 1,774 · see citing works
Suzuki, N., Cheung, T. T., Cai, X.-D., Odaka, A., Otvos, L., Eckman, C., Golde, T. E., & Younkin, S. G. (1994) · Science · doi:10.1126/science.8191290 · Cited by 1,471 · see citing works
Functional gamma‐secretase inhibitors reduce beta‐amyloid peptide levels in brain
Dovey, H. F., John, V., Anderson, J. P., Chen, L. Z., Andrieu, P. D. S. I., Fang, L. Y., Freedman, S. B., Folmer, B., Goldbach, E. G., Holsztynska, E. J., et al. (2001) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2001.00012.x · Cited by 870 · see citing works
Self-citationMa, J., Yee, A., Brewer, H. B., Das, S., & Potter, H. (1994) · Nature · doi:10.1038/372092a0 · Cited by 871 · see citing works
Cellular processing of β-amyloid precursor protein and the genesis of amyloid β-peptide
Haass, C., & Selkoe, D. J. (1993) · Cell · doi:10.1016/0092-8674(93)90312-e · Cited by 833 · see citing works
Amyloid β Protein (Aβ) in Alzheimeri's Disease Brain
Gravina, S. A., Ho, L., Eckman, C. B., Long, K. E., Otvos, L., Younkin, L. H., Suzuki, N., & Younkin, S. G. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.13.7013 · Cited by 676 · see citing works
Ida, N., Hartmann, T., Pantel, J., Schrüder, J., Zerfaß, R., Fürstl, H., Sandbrink, R., Masters, C. L., & Beyreuther, K. T. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.37.22908 · Cited by 476 · see citing works
Cellular mechanisms of β-amyloid production and secretion
Sinha, S., & Lieberburg, I. M. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.20.11049 · Cited by 438 · see citing works
Self-citationAmyloid β protein (Aβ) deposition: Aβ42(43) precedes Aβ40 in down Syndrome
Iwatsubo, T., Mann, D. M. A., Odaka, A., Suzuki, N., & Ihara, Y. (1995) · Annals of Neurology · doi:10.1002/ana.410370305 · Cited by 380 · see citing works
Podlisny, M. B., Ostaszewski, B. L., Squazzo, S. L., Koo, E. H., Rydel, R. E., Teplow, D. B., & Selkoe, D. J. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.16.9564 · Cited by 364 · see citing works
Amyloid β-Peptide Is Transported on Lipoproteins and Albumin in Human Plasma
Biere, A. L., Ostaszewski, B., Stimson, E. R., Hyman, B. T., Maggio, J. E., & Selkoe, D. J. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.51.32916 · Cited by 331 · see citing works
Rapid Communication: Characterization of β‐Amyloid Peptide from Human Cerebrospinal Fluid
Vigo‐Pelfrey, C., Lee, D., Keim, P., Lieberburg, I., & Schenk, D. B. (1993) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1993.tb09841.x · Cited by 385 · see citing works
Self-citationHaass, C., Hung, A. Y., Selkoe, D. J., & Teplow, D. B. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)32503-6 · Cited by 315 · see citing works
Citron, M., Diehl, T. S., Gordon, G., Biere, A. L., Seubert, P. A., & Selkoe, D. J. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.23.13170 · Cited by 287 · see citing works
Citron, M., Vigo-Pelfrey, C., Teplow, D. B., Miller, C., Schenk, D., Johnston, J., Winblad, B., Venizelos, N., Lannfelt, L., & Selkoe, D. J. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.25.11993 · Cited by 366 · see citing works
Exploring the Etiology of Alzheimer Disease Using Molecular Genetics
Lendon, C. L. (1997) · JAMA · doi:10.1001/jama.1997.03540340059034 · Cited by 272 · see citing works
Iwatsubo, T., Saido, T. C., Mann, D. M. A., Lee, V. M.-Y., & Trojanowski, J. Q. (1996) · Europe PMC (PubMed Central) · Cited by 248 · see citing works
The role of Aβ42 in Alzheimer's disease
Younkin, S. G. (1998) · Journal of Physiology-Paris · doi:10.1016/s0928-4257(98)80035-1 · Cited by 282 · see citing works
Gowing, E., Roher, A. E., Woods, A. S., Cotter, R. J., Chaney, M., Little, S. P., & Ball, M. J. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)78080-6 · Cited by 223 · see citing works
Chung, H., Brazil, M. I., Soe, T. T., & Maxfield, F. R. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.45.32301 · Cited by 212 · see citing works
Effects of in vivo conditions on amyloid aggregation
Owen, M. C., Gnutt, D., Gao, M., Wärmländer, S. K. T. S., Jarvet, J., Gräslund, A., Winter, R., Ebbinghaus, S., & Strodel, B. (2019) · Chemical Society Reviews · doi:10.1039/c8cs00034d · Cited by 179 · see citing works
Klafki, H., Abramowski, D., Swoboda, R., Paganetti, P., & Staufenbiel, M. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.45.28655 · Cited by 159 · see citing works
Chromosome Missegregation and Trisomy 21 Mosaicism in Alzheimer's Disease
Geller, L. N., & Potter, H. (1999) · Neurobiology of Disease · doi:10.1006/nbdi.1999.0236 · Cited by 146 · see citing works
Long amyloid beta-protein secreted from wild-type human neuroblastoma IMR-32 cells.
Asami-Odaka, A., Ishibashi, Y., Kikuchi, T., Kitada, C., & Suzuki, N. (1995) · Biochemistry · doi:10.1021/bi00032a022 · Cited by 153 · see citing works
Presenilin function and γ‐secretase activity
Brunkan, A. L., & Goate, A. (2005) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2005.03099.x · Cited by 144 · see citing works
Knops, J., Suomensaari, S., Lee, M., McConlogue, L., Seubert, P. A., & Sinha, S. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.6.2419 · Cited by 115 · see citing works
Self-citationLacosta, A.-M., Pascual-Lucas, M., Pesini, P., Casabona, D., Pérez-Grijalba, V., Marcos-Campos, I., Sarasa, L., Canudas, J., Badi, H., Monleón, I., et al. (2018) · Alzheimer's Research & Therapy · doi:10.1186/s13195-018-0340-8 · Cited by 111 · see citing works
Shin, R., Ogino, K., Kondo, A., Saido, T. C., Trojanowski, J. Q., Kitamoto, T., & Tateishi, J. (1997) · Journal of Neuroscience · doi:10.1523/jneurosci.17-21-08187.1997 · Cited by 96 · see citing works
In Silico Discovery of β-Secretase Inhibitors
Danzhi Huang, A., Lüthi, U., Kolb, P., Cecchini, M., Barberis, A., & Caflisch, A. (2006) · Journal of the American Chemical Society · doi:10.1021/ja0573108 · Cited by 83 · see citing works
Mak, K., Yang, F., Vinters, H. V., Frautschy, S. A., & Cole, G. M. (1994) · Brain Research · doi:10.1016/0006-8993(94)91725-6 · Cited by 80 · see citing works
Ertekin-Taner, N. (2003) · Human Molecular Genetics · doi:10.1093/hmg/ddg343 · Cited by 80 · see citing works
Alzheimer Aβ neurotoxicity: Promotion by antichymotrypsin, ApoE4; inhibition by Aβ-related peptides
Ma, J., Brewer, H. B., & Potter, H. (1996) · Neurobiology of Aging · doi:10.1016/0197-4580(96)00112-1 · Cited by 108 · see citing works
Danzhi Huang, A., Lüthi, U., Kolb, P., Edler, K., Cecchini, M., Audetat, S., Barberis, A., & Caflisch, A. (2005) · Journal of Medicinal Chemistry · doi:10.1021/jm050499d · Cited by 80 · see citing works
Design and Synthesis of Statine-Based Cell-Permeable Peptidomimetic Inhibitors of Human β-Secretase
Hom, R. K., Fang, L. Y., Mamo, S., Tung, J. S., Guinn, A. C., Walker, D., Davis, D. L., Gailunas, A. F., Thorsett, E. D., Sinha, S., et al. (2003) · Journal of Medicinal Chemistry · doi:10.1021/jm025619l · Cited by 78 · see citing works
Self-citationPicomolar Amyloid-β Peptides Enhance Spontaneous Astrocyte Calcium Transients
Lee, L., Kosuri, P., & Arancio, O. (2013) · Journal of Alzheimer s Disease · doi:10.3233/jad-130740 · Cited by 73 · see citing works
Magnetic Resonance Spectroscopic Changes in Alzheimer's Diseasea
Pettegrew, J. W., Klunk, W. E., Panchalingam, K., McCLURE, R. J., & Stanley, J. A. (1997) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1997.tb48480.x · Cited by 58 · see citing works
Watson, D. J., Selkoe, D. J., & Teplow, D. B. (1999) · Biochemical Journal · doi:10.1042/bj3400703 · Cited by 57 · see citing works
Suh, J., Kim, M., Yoo, J., Han, J., Paulina, C., & Lim, M. H. (2022) · Coordination Chemistry Reviews · doi:10.1016/j.ccr.2022.214978 · Cited by 48 · see citing works
Greenberg, B., Savage, M. J., Howland, D., Ali, S. M., Siedlak, S. L., Perry, G. L., Siman, R., & Scott, R. W. (1996) · Neurobiology of Aging · doi:10.1016/0197-4580(96)00001-2 · Cited by 45 · see citing works
Knoch, M. E., Gobes, S. M. H., Pavlovska, I., Su, C., Mistlberger, R. E., & Glass, J. D. (2004) · European Journal of Neuroscience · doi:10.1111/j.0953-816x.2004.03371.x · Cited by 45 · see citing works
Emerging roles of N- and C-terminally truncated Aβ species in Alzheimer’s disease
Wirths, O., & Zampar, S. (2019) · Expert Opinion on Therapeutic Targets · doi:10.1080/14728222.2019.1702972 · Cited by 44 · see citing works
Alzheimer’s Disease: Drug Discovery
Huang, X. (2020) · Exon Publications eBooks · doi:10.36255/exonpublications.alzheimersdisease.2020 · Cited by 45 · see citing works
The βA4 Amyloid Precursor Protein Gene and Alzheimer's Disease
Hendriks, L., & Broeckhoven, C. V. (1996) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1996.0006n.x · Cited by 41 · see citing works
Controlling amyloid growth in multiple dimensions
Dong, J., Lu, K., Lakdawala, A. S., Mehta, A. K., & Lynn, D. G. (2006) · Amyloid · doi:10.1080/13506120600960809 · Cited by 41 · see citing works
Secretases as therapeutic targets for the treatment of Alzheimer's disease
Dominguez, D. I., Strooper, B. D., & Annaert, W. G. (2001) · Amyloid · doi:10.3109/13506120109007356 · Cited by 33 · see citing works
Smith, C. J., & Anderton, B. H. (1994) · Neuropathology and Applied Neurobiology · doi:10.1111/j.1365-2990.1994.tb00977.x · Cited by 27 · see citing works
Kawooya, J. K., Emmons, T. L., Gonzalez‐DeWhitt, P. A., Camp, M. C., & D’Andrea, S. C. (2003) · Analytical Biochemistry · doi:10.1016/j.ab.2003.08.027 · Cited by 26 · see citing works
Greenberg, B. (1995) · Amyloid · doi:10.3109/13506129509036927 · Cited by 25 · see citing works
Takahashi, H., Fukumoto, H., Maeda, R., Terauchi, J., Kato, K., & Miyamoto, M. (2010) · Brain Research · doi:10.1016/j.brainres.2010.09.032 · Cited by 24 · see citing works
Kametania, F., Tanaka, K., Tokuda, T., & Ikeda, S. (1994) · FEBS Letters · doi:10.1016/0014-5793(94)00851-5 · Cited by 20 · see citing works
Molecular Pathology of Alzheimer's Disease
Castellani, R. J., & Perry, G. L. (2013) · Colloquium Series on Neurobiology of Alzheimer s Disease · doi:10.4199/c00095ed1v01y201310alz001 · Cited by 19 · see citing works
Is pharmacological prevention of Alzheimer's a realistic goal?
Anthony, J. C., Breitner, J. C. S., & Zandi, P. P. (2002) · Expert Opinion on Pharmacotherapy · doi:10.1517/14656566.3.4.365 · Cited by 18 · see citing works
Secretases as targets for drug design in Alzheimer’s disease
Hendriksen, J. V. R. B., Nottet, H. S. L. M., & Smits, H. A. (2002) · European Journal of Clinical Investigation · doi:10.1046/j.1365-2362.2002.00937.x · Cited by 17 · see citing works
Immunotherapy Targeting Amyloid-ß Peptides in Alzheimer’s Disease
Zampar, S., & Wirths, O. (2020) · Exon Publications eBooks · doi:10.36255/exonpublications.alzheimersdisease.2020.ch2 · Cited by 17 · see citing works
Younkin, S. G. (1998) · Springer eBooks · doi:10.1007/978-3-642-72103-8_4 · Cited by 15 · see citing works
Zimbone, S., Giuffrida, M. L., Sabatino, G., Natale, G. D., Tosto, R., Consoli, G. M. L., Milardi, D., Pappalardo, G., & Sciacca, M. F. M. (2023) · ACS Chemical Neuroscience · doi:10.1021/acschemneuro.2c00720 · Cited by 15 · see citing works
Artifactual strain-specific signs of incipient brain amyloidosis in APP transgenic mice
Ali, S. M., Siedlak, S. L., Gonzalez‐DeWhitt, P. A., Altman, R. A., Glendening, J. M., Lowery, D. E., Savage, M. J., Polities, H. G., Perry, G. L., & Greenberg, B. (1996) · Neurobiology of Aging · doi:10.1016/0197-4580(95)02065-9 · Cited by 8 · see citing works
Strategic Thoughts on the Alzheimer’s Disease Amyloid Protein Precursor: The Way Forward
Masters, C. L., & Beyreuther, K. T. (1994) · Springer eBooks · doi:10.1007/978-3-662-01135-5_1 · Cited by 7 · see citing works
Hamazaki, H. (1998) · FEBS Letters · doi:10.1016/s0014-5793(98)00157-4 · Cited by 6 · see citing works
Alzheimer’s Disease and Hemorrhagic Stroke: Their Relationship to βA4 Amyloid Deposition
Hendriks, L., Cras, P. P., Martin, J., & Broeckhoven, C. V. (1995) · Research and perspectives in Alzheimer's disease · doi:10.1007/978-3-642-79423-0_4 · Cited by 5 · see citing works
Kuentzel, S. L., Gonzalez‐DeWhitt, P. A., Lowery, D. E., Altman, R. A., Leone, J. W., Heinrikson, R. L., Greenberg, B., & Raub, T. J. (1996) · Amyloid · doi:10.3109/13506129609014360 · Cited by 4 · see citing works
The metabolism of the amyloid precursor protein and its relevance to Alzheimer's disease
Sinha, S. (1995) · Perspectives in Drug Discovery and Design · doi:10.1007/bf02172030 · Cited by 1 · see citing works
Self-citationWang, L., & Campioni, S. (2011) · InTech eBooks · doi:10.5772/20922
Molecular and cell biological aspects of Alzheimer's ß-amyloid precursor and related proteins
van den Hurk, W. H. (2001) · Radboud Repository (Radboud University)
Pathological Changes in the Elderly Human Brain
Mann, D. M. A. (1997) · Springer eBooks · doi:10.1007/978-1-4615-6001-2_2
Mann, D. M. A. (1997) · Springer eBooks · doi:10.1007/978-1-4615-6001-2_4
Siman, R., & Greenberg, B. (1996) · Humana Press eBooks · doi:10.1007/978-1-59259-466-5_10
GXGD-Type Intramembrane Proteases
Steiner, H., & Haass, C. (2007) · Springer eBooks · doi:10.1007/978-1-4020-6311-4_3
Time-Dependent Psychotropic Drug Effects
Nagayama, H., Cornélissen, G., Pandi-Perumal, S. R., & Franz Halberg, A. (2006) · Springer eBooks · doi:10.1007/0-387-27682-3_7
- Authors
- Dovey, H. F., Suomensaari-Chrysler, S., Lieberburg, I., Sinha, S., & Keim, P. S.
- Venue
- NeuroReport
- Published
- 1993-08
- Volume
- 4
- Issue
- 8
- Pages
- 1039-1042
- ISSN
- 0959-4965 (ISSN-L)
- OpenAlex
- W44439419
- Cited by
- 71
- References
- 0
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 1 · 2013: 2
- FWCI
- 7.11
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroreport (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- familial Alzheimer's disease, amyloid-beta, amyloid precursor protein, APP mutations, amyloid-beta 42, Aβ40, conditioned medium, peptide characterization, Alzheimer's disease
- MeSH terms
- Alzheimer Disease, Amino Acid Sequence, Cell Line, Chromatography, Enzyme-Linked Immunosorbent Assay, Humans, Immunologic Techniques, Molecular Sequence Data, Mutation, Mass Spectrometry, Amyloid beta-Peptides
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review