The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Neurite branching on deformable substrates
Open accessAbstract
The mechanical properties of substrates underlying cells can have profound effects on cell structure and function. To examine the effect of substrate deformability on neuronal cell growth, protein-laminated polyacrylamide gels were prepared with differing amounts of bisacrylamide to generate substrates of varying deformability with elastic moduli ranging from 500 to 5500 dyne/cm. Mouse spinal cord primary neuronal cells were plated on the gels and allowed to grow and extend neurites for several weeks in culture. While neurons grew well on the gels, glia, which are normally co-cultured with the neurons, did not survive on these deformable substrates even though the chemical environment was permissive for their growth. Substrate flexibility also had a significant effect on neurite branching. Neurons grown on softer substrates formed more than three times as many branches as those grown on stiffer gels. These results show that mechanical properties of the substrate specifically direct the formation of neurite branches, which are critical for appropriate synaptic connections during development and regeneration.
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Cites
Cell Movement Is Guided by the Rigidity of the Substrate
Lo, C.-M., Wang, H.-B., Dembo, M., & Wang, Y.-L. (2000) · Biophysical Journal · doi:10.1016/s0006-3495(00)76279-5 · Cited by 3,289
Cell locomotion and focal adhesions are regulated by substrate flexibility
Pelham, R. J., & Wang, Y.-L. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.25.13661 · Cited by 3,027
Spinal Cord Repair in Adult Paraplegic Rats: Partial Restoration of Hind Limb Function
Cheng, H., Cao, Y., & Olson, L. (1996) · Science · doi:10.1126/science.273.5274.510 · Cited by 744
Regional, Directional, and Age-Dependent Properties of the Brain Undergoing Large Deformation
Prange, M. T., & Margulies, S. S. (2002) · Journal of Biomechanical Engineering · doi:10.1115/1.1449907 · Cited by 635
Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures
Balgude, A. (2001) · Biomaterials · doi:10.1016/s0142-9612(00)00350-1 · Cited by 518
Arora, P. D., Narani, N., & McCulloch, C. A. G. (1999) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)65334-5 · Cited by 467
Axon Branching Requires Interactions between Dynamic Microtubules and Actin Filaments
Dent, E. W., & Kalil, K. (2001) · The Journal of Neuroscience · doi:10.1523/jneurosci.21-24-09757.2001 · Cited by 397
Wang, Y.-L., & Pelham, R. J. (1998) · Methods in Enzymology · doi:10.1016/s0076-6879(98)98041-7 · Cited by 372
Axonal growth in response to experimentally applied mechanical tension
Bray, D. (1984) · Developmental Biology · doi:10.1016/0012-1606(84)90202-1 · Cited by 369
Slit proteins: key regulators of axon guidance, axonal branching, and cell migration
Brose, K., & Tessier-Lavigne, M. (2000) · Current Opinion in Neurobiology · doi:10.1016/s0959-4388(99)00066-5 · Cited by 359
Cited by
Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
Discher, D. E., Janmey, P., & Wang, Y.-L. (2005) · Science · doi:10.1126/science.1116995 · Cited by 6,382
Self-citationHydrogels with tunable stress relaxation regulate stem cell fate and activity
Chaudhuri, O., Gu, L., Klumpers, D., Darnell, M., Bencherif, S. A., Weaver, J. C., Huebsch, N., Lee, H.-P., Lippens, E., Duda, G. N., et al. (2016) · Nature Materials · doi:10.1038/nmat4489 · Cited by 2,443
Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
Yeung, T., Georges, P. C., Flanagan, L. A., Marg, B., Ortiz, M., Funaki, M., Zahir, N., Ming, W., Weaver, V., & Janmey, P. A. (2005) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.20041 · Cited by 2,325
Self-citationPreparation of Hydrogel Substrates with Tunable Mechanical Properties
Tse, J. R., & Engler, A. J. (2010) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb1016s47 · Cited by 1,070
Metadata
- Authors
- Flanagan, L. A., Ju, Y.-E., Marg, B., Osterfield, M., & Janmey, P. A.
- Venue
- NeuroReport
- Published
- 2002-12
- Volume
- 13
- Issue
- 18
- Pages
- 2411-2415
- ISSN
- 0959-4965 (ISSN-L)
- OpenAlex
- W2095301611
- Cited by
- 759
- References
- 29
- FWCI
- 3.66
- Type
- journal article
- Language
- en
- Open access
- green
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- Flanagan, L. A., Ju, Y.-E., Marg, B., Osterfield, M., & Janmey, P. A. (2002). Neurite branching on deformable substrates. NeuroReport. https://doi.org/10.1097/00001756-200212200-00007
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
