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Cited by 591Pancreatic and Hepatic Oncology ResearchPancreatitis Pathology and TreatmentCancer Cells and Metastasis

Desmoplastic Reaction in Pancreatic Cancer: Role of Pancreatic Stellate Cells

Apte, M. V., Park, S., Phillips, P. A., Santucci, N., Goldstein, D., Kumar, R. K., Ramm, G. A., Buchler, M. +6 more·2004·Pancreas
DOI 10.1097/00006676-200410000-00002
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Abstract

OBJECTIVES: Pancreatic cancer has a very poor prognosis, largely due to its propensity for early local and distant spread. Histopathologically, most pancreatic cancers are characterized by a prominent stromal/fibrous reaction in and around tumor tissue. The aims of this study were to determine whether (1) the cells responsible for the formation of the stromal reaction in human pancreatic cancers are activated pancreatic stellate cells (PSCs) and (2) an interaction exists between pancreatic cancer cells and PSCs that may facilitate local and distant invasion of tumor. METHODS: Serial sections of human pancreatic cancer tissue were stained for desmin and glial fibrillary acidic protein (stellate cell selective markers) and alpha-smooth muscle actin (alphaSMA), a marker of activated PSC activation, by immunohistochemistry, and for collagen using Sirius Red. Correlation between the extent of positive staining for collagen and alphaSMA was assessed by morphometry. The cellular source of collagen in stromal areas was identified using dual staining methodology, ie, immunostaining for alphaSMA and in situ hybridization for procollagen alpha1I mRNA. The possible interaction between pancreatic cancer cells and PSCs was assessed in vitro by exposing cultured rat PSCs to control medium or conditioned medium from 2 pancreatic cancer cell lines (PANC-1 and MiaPaCa-2) for 24 hours. PSC activation was assessed by cell proliferation and alphaSMA expression. RESULTS: Stromal areas of human pancreatic cancer stained strongly positive for the stellate cell selective markers desmin and GFAP (indicating the presence of PSCs), for alphaSMA (suggesting that the PSCs were in their activated state) and for collagen. Morphometric analysis demonstrated a close correlation (r = 0.77; P < 0.04; 8 paired sections) between the extent of PSC activation and collagen deposition. Procollagen mRNA expression was localized to alphaSMA-positive cells in stromal areas indicating that activated PSCs were the predominant source of collagen in stromal areas. Exposure of PSCs to pancreatic cancer cell secretions in vitro resulted in PSC activation as indicated by significantly increased cell proliferation and alphaSMA expression. CONCLUSIONS: Activated PSCs are present in the stromal reaction in pancreatic cancers and are responsible for the production of stromal collagen. PSC function is influenced by pancreatic cancer cells. Interactions between tumor cells and stromal cells (PSCs) may play an important role in the pathobiology of pancreatic cancer.

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Crossref · 10.1097/00006676-200410000-00002

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  • The normal pancreas contains heterogeneous fibroblast populations, including pancreatic stellate cells, perivascular fibroblasts, and other parenchymal fibroblasts.

    “The normal pancreas contains heterogeneous fibroblasts populations (Fig. 2) including pancreatic stellate cells —similar to liver stellate cells and containing vitamin A-rich lipid droplets141— perivascular fibroblasts, and other parenchymal fibroblasts.”

    From Pancreatic cancer: Advances and challenges (2023) · cites this paper as [141]

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Authors
Apte, M. V., Park, S., Phillips, P. A., Santucci, N., Goldstein, D., Kumar, R. K., Ramm, G. A., Buchler, M., Friess, H., McCarroll, J. A., Keogh, G., Merrett, N., Pirola, R., & Wilson, J. S.
Affiliations
  • Bankstown Lidcombe Hospital (AU)
  • UNSW Sydney (AU)
  • QIMR Berghofer Medical Research Institute (AU)
  • Heidelberg University (DE)
Corresponding author
  • Apte, M. V.
  • Park, S.
  • Phillips, P. A.
  • Santucci, N.
  • McCarroll, J. A.
  • Keogh, G.
  • Merrett, N.
  • Wilson, J. S.
ORCID
Venue
Pancreas
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2004-10
Volume
29
Issue
3
Pages
179-187
ISSN
0885-3177 (ISSN-L)
DOI
10.1097/00006676-200410000-00002
OpenAlex
W2013093033
Cited by
591
References
35
Citations by year
2026: 9 · 2025: 16 · 2024: 19 · 2023: 34 · 2022: 45 · 2021: 48 · 2020: 44 · 2019: 31 · 2018: 43 · 2017: 47 · 2016: 44 · 2015: 35 · 2014: 37 · 2013: 29 · 2012: 24
FWCI
2.73
Type
journal article
Language
en
Open access
closed
Locations
  • Pancreas (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Oncology › Pancreatic and Hepatic Oncology Research
  • Health Sciences › Medicine › Surgery › Pancreatitis Pathology and Treatment
  • Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
Keywords
desmoplasia, pancreatic stellate cells, tumor stroma, collagen deposition, alpha-smooth muscle actin, stellate cell activation, tumor-stroma interactions, tumor invasion, desmin, glial fibrillary acidic protein
MeSH terms
Actins, Animals, Cell Division, Cells, Cultured, Collagen, Desmin, Glial Fibrillary Acidic Protein, Humans, Neoplasm Invasiveness, Neoplasm Proteins, Pancreas, Pancreatic Neoplasms, RNA, Messenger, Biomarkers, Tumor, Culture Media, Conditioned, Stromal Cells, Cell Line, Tumor, Rats

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