CoChat
Sign Up
Cited by 167Atherosclerosis and Cardiovascular DiseasesLipoproteins and Cardiovascular HealthCholesterol and Lipid Metabolism

The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.

The Apolipoprotein E Knockout Mouse: A Model Documenting Accelerated Atherogenesis In Uremia

Buzello, Moriz, Törnig, Johannes, Faulhaber, Jörg, Ehmke, Heimo, Ritz, Eberhard, Amann, Kerstin·2003·Journal of the American Society of Nephrology
DOI 10.1097/01.asn.0000045048.71975.fc
Open this paper in CoChat — free account

Abstract

Rodents do not develop spontaneous atherosclerosis. Currently, there is no good animal model to study the effect of uremia on atherosclerosis. This study evaluated whether apolipoprotein E knockout (Apoe-/-) mice are useful to study the effect of renal dysfunction on cardiovascular risk. Apoe-/- mice have decreased serum apolipoprotein E and exhibit lipid abnormalities and atherosclerosis even on a low-cholesterol diet. Ten-wk-old Apoe-/- mice were subtotally nephrectomised (SNX Apoe-/-; n = 8), uninephrectomised (UNX Apoe-/-; n = 5), or sham-operated (sham Apoe-/-; n = 5) and compared with their genetic controls (SNX C57/BL6; UNX C57/BL6; sham C57/BL6). After 12 wk, BP was measured intraarterially, blood samples were taken, and the experiment was terminated by perfusion fixation. The heart weight was determined, and quantitative morphologic analysis of intramyocardial arteries and aortic changes was performed. At the end of the experiment, heart weight and relative left ventricular weight were comparable in all groups. Intraarterial BP was somewhat higher in Apoe-/- mice compared with controls. Baseline serum cholesterol and triglyceride levels were higher in Apoe-/- mice than in C57/BL6. Atherosclerotic plaques were not present in sham or UNX C57/BL6, but minor plaque formation was noted in some SNX control animals. In contrast, beginning plaques were seen even in untouched Apoe-/- mice, and strikingly increased plaque formation was noted in UNX and SNX Apoe-/- mice. Maximal plaque diameter (cross-section) was 37 +/- 74 micro m in SNX C57/BL6, 191 +/- 90 micro m in sham Apoe-/-, 323 +/- 66 micro m in UNX Apoe-/-, and 457 +/- 17 micro m in SNX Apoe-/-. The plaque morphology corresponded with that of early plaques characterized by foam cells and virtual absence of lymphocytes or smooth muscle cell infiltration. In conclusion, even mild renal dysfunction, i.e., after uninephrectomy, causes a dramatic increase in plaque size and aggressive morphology (foam cell rich soft plaques) in the animal model of the Apoe-/- mouse.

Overview

Integrity signals

Registry

Crossref · 10.1097/01.asn.0000045048.71975.fc

Cites

16 of 16 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 16 references

Cited by

Sorted by citations

Related

See all

Metadata

Authors
Buzello, M., Törnig, J., Faulhaber, J., Ehmke, H., Ritz, E., & Amann, K.
Affiliations
  • Friedrich-Alexander-Universität Erlangen-Nürnberg (DE)
  • Heidelberg University (DE)
Corresponding author
Amann, Kerstin
ORCID
Venue
Journal of the American Society of Nephrology
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2003-02
Volume
14
Issue
2
Pages
311-316
ISSN
1046-6673 (ISSN-L)
DOI
10.1097/01.asn.0000045048.71975.fc
OpenAlex
W2139687501
Cited by
167
References
16
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 18 · 2022: 4 · 2021: 5 · 2020: 3 · 2019: 3 · 2018: 2 · 2017: 2 · 2016: 3 · 2015: 9 · 2014: 8 · 2013: 13 · 2012: 2
FWCI
4.44
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of the American Society of Nephrology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Else Kröner-Fresenius-Stiftung
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipoproteins and Cardiovascular Health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Cholesterol and Lipid Metabolism
Keywords
ApoE-deficient mice, atherosclerosis, renal dysfunction, unilateral nephrectomy, subtotal nephrectomy, uremia, atherosclerotic plaque formation, plaque morphology, foam cells, serum cholesterol, triglycerides, blood pressure, cardiovascular risk, cholesterol-lowering diet, lipid metabolism disorders, heart weight, left ventricular hypertrophy
MeSH terms
Animals, Apolipoproteins E, Arteries, Arteriosclerosis, Blood Pressure, Elasticity, Mice, Inbred C57BL, Nephrectomy, Time Factors, Uremia, Mice, Knockout, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review