Assessment of the number of lamellae in the central region of the normal human corneal stroma at the resolution of the transmission electron microscope
Abstract
Purpose To determine the total number of lamellae within the central region of the human corneal stroma by using a continuous section through the corneal stroma and at the ultrastructural resolution of the electron microscope. Methods Six human eye bank corneas, from individuals aged between 42 and 82 years, were received in corneal storage medium (Optisol GS) and then processed for transmission electron microscopy with buffered 2% glutaraldehyde fixation. Thin sections were mounted on parallel bar copper grids for an uninterrupted full corneal thickness observation. A sequence of overlapping micrographs were taken at 2,600x magnification, printed at a final magnification of 3,640x, and assembled as a montage. The number of lamellae were counted across the corneal stromal strip by three observers, independently, by using a preagreed set of criteria for identifying individual lamella. Results The average number of lamellae per stroma was determined to be 242+/-4 (range, 234-247). In the more anterior region of the stroma, the density of the lamellae was 50% greater than in the posterior stroma (mean, 57+/-12 per 100 microm anteriorly vs. 38+/-5 per 100 microm posteriorly). Interobserver differences were modest and generally less than the intersample variance. Conclusions When assessed at the resolution of the transmission electron microscope, the number of stromal lamellae in the central region of the human cornea are higher (at approximately 240 per cornea) than most previous…
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At the centre of the human cornea there are approximately 200 lamellae through the thickness.
“At the centre of the human cornea there are approximately 200 lamellae through the thickness”
From Corneal structure and transparency (2015) · cites this paper as Bergmanson et al., 2005
The packing density of lamellae is higher in the anterior lamella than in the posterior ones.
“the packing density is higher in the anterior lamella than in the posterior ones (Bergmanson et al., 2005)”
From Corneal structure and transparency (2015) · cites this paper as Bergmanson et al., 2005
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Corneal structure and transparency
Meek, K. M., & Knupp, C. (2015) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2015.07.001 · Cited by 853 · see citing works
- Authors
- Bergmanson, J. P. G., Horne, J., Doughty, M. J., Garcia, M., & Gondo, M.
- Affiliations
- University of Houston (US)
- Glasgow Caledonian University (GB)
- Corresponding author
- Bergmanson, Jan P.G.
- Venue
- Eye & Contact Lens: Science & Clinical Practice
- Published
- 2005-11
- Volume
- 31
- Issue
- 6
- Pages
- 281-287
- ISSN
- 0733-8902 (ISSN-L)
- 1542-2321
- OpenAlex
- W2025385325
- Cited by
- 75
- References
- 0
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 10 · 2023: 5 · 2022: 8 · 2021: 3 · 2020: 2 · 2019: 5 · 2018: 5 · 2017: 4 · 2016: 1 · 2015: 5 · 2014: 1 · 2012: 1
- FWCI
- 1.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Eye & Contact Lens Science & Clinical Practice (publishedVersion)
- PubMed (publishedVersion)
- ResearchOnline (acceptedVersion)
- ResearchOnline (Glasgow Caledonian University) (submittedVersion)
- Funders
- National Eye Institute (P30 EY-07751)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Corneal surgery and disorders
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Corneal Surgery and Treatments
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ocular Surface and Contact Lens
- Keywords
- corneal stroma, lamellae, corneal ultrastructure, corneal thickness, interobserver variability, human cornea
- MeSH terms
- Adult, Aged, Aged, 80 and over, Collagen, Corneal Stroma, Eye Banks, Humans, Middle Aged, Observer Variation, Corneal Transplantation, Microscopy, Electron, Transmission
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