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Bio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray microspectroscopy
Open accessAbstract
The direct detection of biologically relevant metals in single cells and of their speciation is a challenging task that requires sophisticated analytical developments. The aim of this article is to present the recent achievements in the field of cellular chemical element imaging, and direct speciation analysis, using proton and synchrotron radiation X-ray micro- and nano-analysis. The recent improvements in focusing optics for MeV-accelerated particles and keV X-rays allow application to chemical element analysis in subcellular compartments. The imaging and quantification of trace elements in single cells can be obtained using particle-induced X-ray emission (PIXE). The combination of PIXE with backscattering spectrometry and scanning transmission ion microscopy provides a high accuracy in elemental quantification of cellular organelles. On the other hand, synchrotron radiation X-ray fluorescence provides chemical element imaging with less than 100 nm spatial resolution. Moreover, synchrotron radiation offers the unique capability of spatially resolved chemical speciation using micro-X-ray absorption spectroscopy. The potential of these methods in biomedical investigations will be illustrated with examples of application in the fields of cellular toxicology, and pharmacology, bio-metals and metal-based nano-particles.
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“The combination of X-ray imaging with X-ray Fluorescence microscopy (μ-XRF) allows monitoring of the distribution of elements in tissue samples without artefacts2728, as demonstrated in our recent study of asbestos bodies29.”
From The interaction of asbestos and iron in lung tissue revealed by synchrotron-based scanning X-ray microscopy (2013) · cites this paper as [27]
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Metadata
- Authors
- Ortega, R., Devès, G., & Carmona, A.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Venue
- Journal of The Royal Society Interface
- Publisher
- The Royal Society
- Published
- 2009-07-15
- Volume
- 6
- Issue
- suppl_5
- Pages
- S649-58
- ISSN
- 1742-5662 (ISSN-L)
- 1742-5689
- PMC
- PMC2843977
- OpenAlex
- W2167807896
- Cited by
- 150
- References
- 62
- Citations by year
- 2025: 5 · 2024: 2 · 2023: 7 · 2022: 5 · 2021: 3 · 2020: 5 · 2019: 13 · 2018: 6 · 2017: 7 · 2016: 17 · 2015: 9 · 2014: 18 · 2013: 6 · 2012: 23
- FWCI
- 2.20
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of The Royal Society Interface (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Oskar-Bordeaux (Universite de Bordeaux) (submittedVersion)
- Topics
- Physical Sciences › Physics and Astronomy › Radiation › X-ray Spectroscopy and Fluorescence Analysis
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Electron and X-Ray Spectroscopy Techniques
- Physical Sciences › Physics and Astronomy › Radiation › Advanced X-ray Imaging Techniques
- Keywords
- metal speciation, PIXE, backscattering spectrometry, scanning transmission ion microscopy, synchrotron X-ray fluorescence, micro-X-ray absorption spectroscopy, metal-based nanoparticles
- MeSH terms
- Biopolymers, Metals, Protons, Spectrometry, X-Ray Emission, Synchrotrons
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