Critical thermal limits depend on methodological context
Open accessAbstract
A full-factorial study of the effects of rates of temperature change and start temperatures was undertaken for both upper and lower critical thermal limits (CTLs) using the tsetse fly,Glossina pallidipes. Results show that rates of temperature change and start temperatures have highly significant effects on CTLs, although the duration of the experiment also has a major effect. Contrary to a widely held expectation, slower rates of temperature change (i.e. longer experimental duration) resulted in poorer thermal tolerance at both high and low temperatures. Thus, across treatments, a negative relationship existed between duration and upper CTL while a positive relationship existed between duration and lower CTL. Most importantly, for predicting tsetse distribution,G. pallidipes suffer loss of function at less severe temperatures under the most ecologically relevant experimental conditions for upper (0.06°C min−1; 35°C start temperature) and lower CTL (0.06°C min−1; 24°C start temperature). This suggests that the functional thermal range of G. pallidipes in the wild may be much narrower than previously suspected, approximately 20–40°C, and highlights their sensitivity to even moderate temperature variation. These effects are explained by limited plasticity of CTLs in this species over short time scales. The results of the present study have broad implications for understanding temperature tolerance in these and other terrestrial arthropods.
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Methodological differences in ramping rate and starting temperature affect the duration of heat tolerance trials and the resultant heat tolerance.
“Methodological differences in ramping rate and starting temperature, which affect the duration of heat tolerance trials, have been shown to affect the resultant heat tolerance (Terblanche et al., 2007).”
From It's about time: Linkages between heat tolerance, thermal acclimation and metabolic rate at different temporal scales in the freshwater amphipod Gammarus fossarum Koch, 1836 (2018) · cites this paper as Terblanche et al., 2007
Explicitly including time in heat tolerance assays gives a more complete picture of the effect of thermal acclimation.
“Thus, not only can the duration affect the outcome of assays of heat tolerance (Terblanche et al., 2007), but explicitly including time in these assays also gives a more complete picture of the effect of thermal acclimation.”
From It's about time: Linkages between heat tolerance, thermal acclimation and metabolic rate at different temporal scales in the freshwater amphipod Gammarus fossarum Koch, 1836 (2018) · cites this paper as Terblanche et al., 2007
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“Methodological differences in ramping rate and starting temperature, which affect the duration of heat tolerance trials, have been shown to affect the resultant heat tolerance (Terblanche et al., 2007).”
BackgroundKoopman, K. R., Collas, F. P. L., van der Velde, G., & Verberk, W. C. E. P. (2016) · Hydrobiologia · doi:10.1007/s10750-015-2386-y · Cited by 47 · see citing works
- Authors
- Terblanche, J. S., Deere, J. A., Clusella-Trullas, S., Janion, C., & Chown, S. L.
- Affiliations
- Stellenbosch University (ZA)
- Centre for Invasion Biology, Department of Botany and Zoology, Stellenbosch UniversityPrivate Bag X1, Matieland 7602, Republic of South Africa
- Venue
- Proceedings of the Royal Society B: Biological Sciences
- Publisher
- The Royal Society
- Published
- 2007-09-18
- Volume
- 274
- Issue
- 1628
- Pages
- 2935-2943
- ISSN
- 0080-4649 (ISSN-L)
- 0962-8452
- 1471-2954
- PMC
- PMC2291155
- OpenAlex
- W2168788964
- Cited by
- 532
- References
- 64
- Citations by year
- 2026: 26 · 2025: 26 · 2024: 36 · 2023: 42 · 2022: 39 · 2021: 40 · 2020: 46 · 2019: 36 · 2018: 34 · 2017: 33 · 2016: 30 · 2015: 20 · 2014: 22 · 2013: 12 · 2012: 31
- FWCI
- 11.85
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the Royal Society B Biological Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- SUNScholar (Stellenbosch University) (submittedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (R01AI052456, AI 52456)
- Topics
- Physical Sciences › Environmental Science › Ecology › Physiological and biochemical adaptations
- Health Sciences › Medicine › Physiology › Thermoregulation and physiological responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Insect and Arachnid Ecology and Behavior
- Keywords
- critical thermal limits, Glossina pallidipes, tsetse flies, heat tolerance, temperature change rate, experimental duration, thermal plasticity, terrestrial arthropods
- MeSH terms
- Acclimatization, Animals, Body Temperature Regulation, Temperature, Time Factors, Tsetse Flies
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