The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Fluorometric Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization in Microdilution Wells as an Alternative to Membrane Filter Hybridization in which Radioisotopes Are Used To Determine Genetic Relatedness among Bacterial Strains
Abstract
Fluorometric hybridization in microdilution wells was developed to determine genetic relatedness among microorganisms. Total chromosomal deoxyribonucleic acid (DNA) for hybridization reactions was labeled with photoreactive biotin (photobiotin). The biotinylated DNA was hybridized with single-stranded unlabeled DNAs which had been immobilized on the surfaces of microdilution wells. After hybridization, biotinylated DNA was quantitatively detected with beta-D-galactosidase and a fluorogenic substrate, 4-methylumbelliferyl-beta-D-galactopyranoside. Homology values obtained with this fluorometric direct binding method were compared with values obtained with two membrane filter methods, one in which photobiotin labeling was used and one in which radioisotope labeling was used. The results showed that the fluorometric direct binding method in which microdilution wells are used could be an alternative to radioisotope and membrane filter hybridization methods.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Feinberg, A. P., & Vogelstein, B. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90418-9 · Cited by 24,887
Rigby, P. W. J., Dieckmann, M., Rhodes, C., & Berg, P. (1977) · Journal of Molecular Biology · doi:10.1016/0022-2836(77)90052-3 · Cited by 11,401
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
(1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90381-6 · Cited by 4,915
Bergey's manual of systematic bacteriology
(1987) · Gene · doi:10.1016/0378-1119(87)90414-8 · Cited by 3,973
The Quantitative Measurement of DNA Hybridization from Renaturation Rates
Ley, J. D., Cattoir, H., & Reynaerts, A. (1970) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1970.tb00830.x · Cited by 2,595
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane
Gillespie, D., & Spiegelman, S. (1965) · Journal of Molecular Biology · doi:10.1016/s0022-2836(65)80331-x · Cited by 1,964
Hybridization of nucleic acids immobilized on solid supports
Meinkoth, J., & Wahl, G. (1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90808-x · Cited by 1,324
Leary, J. J., Brigati, D. J., & Ward, D. C. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.13.4045 · Cited by 1,060
Crosa, J. H., Brenner, D. J., & Falkow, S. (1973) · Journal of Bacteriology · doi:10.1128/jb.115.3.904-911.1973 · Cited by 307
A colorimetric method for DNA hybridization
Renz, M., & Kurz, C. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.8.3435 · Cited by 292
Cited by
DNA–DNA hybridization values and their relationship to whole-genome sequence similarities
Goris, J., Konstantinidis, K. T., Klappenbach, J. A., Coenye, T., Vandamme, P., & Tiedje, J. M. (2007) · International Journal of Systematic and Evolutionary Microbiology · doi:10.1099/ijs.0.64483-0 · Cited by 4,750
KAWAMURA, Y., HOU, X.-G., SULTANA, F., MIURA, H., & EZAKI, T. (1995) · International Journal of Systematic Bacteriology · doi:10.1099/00207713-45-2-406 · Cited by 503
Self-citationSuzuki, M., Nakagawa, Y., Harayama, S., & Yamamoto, S. (2001) · International Journal of Systematic and Evolutionary Microbiology · doi:10.1099/00207713-51-5-1639 · Cited by 396
Atomi, H., Fukui, T., Kanai, T., Morikawa, M., & Imanaka, T. (2004) · Archaea · doi:10.1155/2004/204953 · Cited by 304
Mukai, T., Asasaka, T., Sato, E., Mori, K., Matsumoto, M., & Ohori, H. (2002) · FEMS Immunology & Medical Microbiology · doi:10.1111/j.1574-695x.2002.tb00541.x · Cited by 298
Metadata
- Authors
- EZAKI, T., HASHIMOTO, Y., & YABUUCHI, E.
- Venue
- International Journal of Systematic Bacteriology
- Publisher
- Microbiology Society
- Published
- 1989-07-01
- Volume
- 39
- Issue
- 3
- Pages
- 224-229
- ISSN
- 0020-7713 (ISSN-L)
- 1465-2102
- OpenAlex
- W2164463552
- Cited by
- 3,261
- References
- 14
- FWCI
- 7.96
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Fluorometric Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization in Microdilution Wells as an Alternative to Membrane Filter Hybridization in which Radioisotopes Are Used To Determine Genetic Relatedness among Bacterial Strains. https://app.cochat.ai/papers/10.1099/00207713-39-3-224
- The paper
- EZAKI, T., HASHIMOTO, Y., & YABUUCHI, E. (1989). Fluorometric Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization in Microdilution Wells as an Alternative to Membrane Filter Hybridization in which Radioisotopes Are Used To Determine Genetic Relatedness among Bacterial Strains. International Journal of Systematic Bacteriology. https://doi.org/10.1099/00207713-39-3-224
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
