The DOI matches its Crossref record; 9 of the 12 references it cites were found in a registry or an index.
Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp. nov and Clostridium saccharobutylicum sp. nov
Abstract
On the basis of 16S rRNA gene sequencing and DNA-DNA reassociation, industrial solvent-producing clostridia have been assigned to four species. In this study, the phenotypic characteristics of Clostridium acetobutylicum, Clostridium beijerinckii, 'Clostridium saccharoperbutylacetonicum', and an unnamed Clostridium sp. represented by the strains NCP 262T and NRRL B643 are compared. In addition, a further 40 strains of solvent-producing clostridia have been classified by biotyping, DNA fingerprinting and 16S rRNA gene sequencing. These included 14 C. beijerinckii strains, two strains currently designated as 'Clostridium kaneboi' and 'Clostridium butanologenum', and 24 production strains used in the commercial acetone-butanol fermentation. All of the C. beijerinckii strains were confirmed to have been classified correctly. The 'C. kaneboi' and 'C. butanologenum' strains require reclassification as C. acetobutylicum and C. beijerinckii, respectively. The commercial production strains were found to belong either to C. beijerinckii or to the unnamed Clostridium sp. For the comparative phenotypic studies of the four species, representative strains were selected from each of the DNA-fingerprint subgroups within each species. These strains were analysed for their ability to utilize different carbohydrates, hydrolyse gelatin or aesculin, and produce indole, and were tested for the presence of catalase and urease. On the basis of these results, several phenotypic traits were found to be useful for differentiating between the four species. The descriptions of C. acetobutylicum and C. beijerinckii have been emended. The names Clostridium saccharoperbutylacetonicum sp. nov. [type strain = N1-4 (HMT) = ATCC 27021T] and Clostridium saccharobutylicum sp. nov. (type strain = DSM 13864T = ATCC BAA-117T) are proposed for the two new species.
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“Earlier efforts of transposon mutagenesis were demonstrated in C. saccharobutylicum (Keis et al., 2001), C. acetobutylicum, and C. beijerinckii, but issues with multiple transposon insertions per mutant, and non-random distribution of insertions were also reported (Bertram and Dürre, 1989; Woolley et al., 1989).”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Keis et al., 2001
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Acetone-butanol fermentation revisited
Jones, D. T., & Woods, D. (1986) · Microbiological Reviews · doi:10.1128/mr.50.4.484-524.1986 · Cited by 1,966
Self-citationOxygen and the Growth and Metabolism of Clostridium acetobutylicum
O'Brien, R. W., & Morris, J. G. (1971) · Journal of General Microbiology · doi:10.1099/00221287-68-3-307 · Cited by 283
Solvent Production and Morphological Changes in Clostridium acetobutylicum
Jones, D. T., van der Westhuizen, A., Long, S., Allcock, E. R., Reid, S. J., & Woods, D. (1982) · Applied and Environmental Microbiology · doi:10.1128/aem.43.6.1434-1439.1982 · Cited by 175
Self-citationTaxonomy and Phylogeny of Industrial Solvent-Producing Clostridia
Keis, S., Bennett, C., Ward, V. K., & JONES, D. T. (1995) · International Journal of Systematic Bacteriology · doi:10.1099/00207713-45-4-693 · Cited by 124
Self-citationOrigins and relationships of industrial solvent-producing clostridial strains
Jones, D. T., & Keis, S. (1995) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.1995.tb00206.x · Cited by 82
Self-citationJohnson, J. L., Toth, J., Santiwatanakul, S., & Chen, J.-S. (1997) · International Journal of Systematic Bacteriology · doi:10.1099/00207713-47-2-420 · Cited by 77
Shaheen, R., Shirley, M., & Jones, D. T. (2000) · PubMed · Cited by 72
Self-citationWoolley, R. C., & Morris, J. G. (1990) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1990.tb01569.x · Cited by 56
Phenotypic and genotypic differences between certain strains ofClostridium acetobutylicum
Wilkinson, S. R., Young, M., Goodacre, R., Morris, J. G., Farrow, J. A. E., & Collins, M. D. (1995) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1995.tb07358.x · Cited by 34
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Biobutanol: An attractive biofuel
Dürre, P. (2007) · Biotechnology Journal · doi:10.1002/biot.200700168 · Cited by 935
Fermentative production of butanol—the industrial perspective
Green, E. M. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.02.004 · Cited by 705
Developments in biobutanol production: New insights
Kumar, M., & Gayen, K. (2011) · Applied Energy · doi:10.1016/j.apenergy.2010.12.055 · Cited by 487
Liew, F., Martin, M. E., Tappel, R. C., Heijstra, B. D., Mihalcea, C., & Köpke, M. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00694 · Cited by 485
Kim, S., Han, S. K., & Shin, H.-S. (2005) · Process Biochemistry · doi:10.1016/j.procbio.2005.06.013 · Cited by 349
Metadata
- Authors
- Keis, S., Shaheen, R., & Jones, D. T.
- Affiliations
- University of Otago (NZ)
- ORCID
- Shaheen, R
- Venue
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
- Publisher
- Microbiology Society
- Published
- 2001-11-01
- Volume
- 51
- Issue
- 6
- Pages
- 2095-2103
- ISSN
- 1466-5026 (ISSN-L)
- 1466-5034
- OpenAlex
- W1995716143
- Cited by
- 192
- References
- 12
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 6 · 2023: 5 · 2022: 8 · 2021: 9 · 2020: 16 · 2019: 9 · 2018: 9 · 2017: 14 · 2016: 17 · 2015: 13 · 2014: 8 · 2013: 20 · 2012: 6
- FWCI
- 1.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Anaerobic Digestion and Biogas Production
- Keywords
- Clostridium acetobutylicum, Clostridium beijerinckii
- MeSH terms
- Acetone, Butanols, Clostridium, DNA, Ribosomal, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S, Solvents, Bacterial Typing Techniques, DNA Fingerprinting, Sequence Analysis, DNA, Carbohydrate Metabolism
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