Regulation of the interferon system: evidence that Vero cells have a genetic defect in interferon production
Abstract
A clone of Vero cells was isolated and shown to be totally unable to synthesize interferon and insensitive to the toxic effect of poly(rI).poly(rC) treatment. Cells of this clone and mouse L cells were fused by treatment with polyethylene glycol or Sendai virus. Hybrid cell clones were isolated following selection in medium containing hypoxanthine, thymidine and ouabain. The hybrids were sensitive to the antiviral effect of poly(rI).poly(rC) and synthesized mouse, but not primate, interferon. It is proposed that in Vero cells, the gene for interferon synthesis is defective or absent.
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BHK and Vero E6 cells are known to be deficient in interferon signaling, and EMCV is known to be highly sensitive to interferon.
“as both BHK and Vero E6 cells are known to be deficient in interferon signaling [54,55], and EMCV is known to be highly sensitive to interferon [56]”
From Zoological transmission of encephalomyocarditis virus in the United States: Virus evolution, host ecology, and capsid antigenicity derived from an outbreak (2026) · cites this paper as [54]
Cites
Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed Recombinants
Littlefield, J. W. (1964) · Science · doi:10.1126/science.145.3633.709 · Cited by 2,103 · see citing works
Desmyter, J., Melnick, J. L., & Rawls, W. E. (1968) · Journal of Virology · doi:10.1128/jvi.2.10.955-961.1968 · Cited by 502 · see citing works
Jm, N., & Enders, J. F. (1968) · Experimental Biology and Medicine · doi:10.3181/00379727-127-32668 · Cited by 116 · see citing works
Stewart, W. E., Clercq, E. D., Billiau, A., et al. (1972) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.69.7.1851 · Cited by 98 · see citing works
Interferon Messenger RNA: Translation in Heterologous Cells
Maeyer‐Guignard, J. D., Maeyer, E. D., & Montagnier, L. (1972) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.69.5.1203 · Cited by 53 · see citing works
Emeny, J. M., & Morgan, M. J. (1979) · Journal of General Virology · doi:10.1099/0022-1317-43-1-253 · Cited by 18 · see citing works
Self-citationIsolation and Characterization of Virus-resistant Mouse Embryo Fibroblasts
Morgan, M. J., Colby, C., & Hulse, J. L. (1973) · Journal of General Virology · doi:10.1099/0022-1317-20-3-377 · Cited by 15 · see citing works
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Devaraj, S. G., Wang, N., Chen, Z., Chen, Z., Tseng, M., Barretto, N., Lin, R., Peters, C. J., Tseng, C.-T. K., Baker, S. C., et al. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m704870200 · Cited by 411 · see citing works
5’-triphosphate-dependent activation of PKR by RNAs with short stem-loops
Nallagatla, S. R., Hwang, J., Toroney, R., Zheng, X., Cameron, C. E., & Bevilacqua, P. C. (2007) · Science · doi:10.1126/science.1147347 · Cited by 222 · see citing works
Morrison, J. M., & Racaniello, V. R. (2009) · Journal of Virology · doi:10.1128/jvi.02177-08 · Cited by 51 · see citing works
Adhikari, A., Feng, K. H., Shrestha, A., Rodriguez, C. E., Nolan, E. C., Pye, G. W., Gauger, P. C., Denagamage, T., Holmes, E. C., & Allison, A. B. (2026) · PLOS Pathogens · doi:10.1371/journal.ppat.1013861 · Cited by 1 · see citing works
“However, as both BHK and Vero E6 cells are known to be deficient in interferon signaling [54,55], and EMCV is known to be highly sensitive to interferon [56], such cell lines are unlikely to represent accurate models of in vivo viral infection, but do show their utility for EMCV isolation in…”
- Authors
- Emeny, J. M., & Morgan, M. J.
- Affiliations
- University of Leicester (GB)
- Venue
- Journal of General Virology
- Publisher
- Microbiology Society
- Published
- 1979-04-01
- Volume
- 43
- Issue
- 1
- Pages
- 247-252
- ISSN
- 0022-1317 (ISSN-L)
- 1465-2099
- OpenAlex
- W2071467437
- Cited by
- 440
- References
- 7
- Citations by year
- 2026: 35 · 2025: 26 · 2024: 29 · 2023: 31 · 2022: 30 · 2021: 29 · 2020: 37 · 2019: 20 · 2018: 19 · 2017: 11 · 2016: 7 · 2015: 11 · 2014: 14 · 2013: 19 · 2012: 15
- FWCI
- 0.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of General Virology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Keywords
- interferon, interferon production, Vero cells, genetic disorders, antiviral activity, cell fusion, mouse interferon, Sendai virus, L cells, interferon genes
- MeSH terms
- Animals, Haplorhini, Cell Line, Cytopathogenic Effect, Viral, Genes, Humans, Hybrid Cells, Interferons, Kidney, L Cells, Poly I-C, Sindbis Virus
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