Cited by 53Poxvirus research and outbreaksVirology and Viral DiseasesHerpesvirus Infections and Treatments

Identification and Cloning of the Fowlpox Virus Thymidine Kinase Gene Using Vaccinia Virus

Boyle, D. B., Coupar, B. E. H.·1986·Journal of General Virology

Abstract

Using vaccinia virus as a selection and cloning vehicle, a thymidine kinase (TK) gene of fowlpox virus (FPV) has been identified. A plasmid, pF130, containing part of the HindIII-F region of vaccinia virus was used to shotgun clone EcoRI fragments of FPV DNA into TK- vaccinia virus and select for TK+ recombinants. The TK+ recombinant vaccinia virus contained a 5.5 kb EcoRI fragment of FPV. This FPV fragment was cloned into pUC9 and the presence of the TK gene in this fragment was confirmed by its ability to rescue TK+ vaccinia virus from TK- virus, when inserted into pF130. A recombinant vaccinia virus containing this FPV fragment induced TK enzyme activity in the cytoplasm of infected cells. The vaccinia virus RNA polymerase appeared able to recognize the FPV promoter sequences of the FPV TK gene since the fragment operated in the marker rescue, irrespective of its orientation to the vaccinia virus promoter in pF130. Using restriction enzyme analysis, insertion of subfragments of the 5.5 kb FPV fragment into pF130 and marker rescue, we were able to map the position of the TK gene in the 5.5 kb EcoRI fragment. This approach may facilitate identification and cloning of TK genes from other poxviruses.

Integrity signals

Registry

Crossref · 10.1099/0022-1317-67-8-1591

Cites

31 of 31 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Companion cell lines with enhanced lung colonization capacity have enabled study of metastasis-driving mechanisms.

    “Companion cell lines with enhanced lung colonization capacity, such as SaOS2-LM7, MG63.3 and 143B 209,210,211 , have enabled the study of metastasis-driving mechanisms 50,212 .”

    From Osteosarcoma (2022) · cites this paper as [211]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Boyle, D. B., & Coupar, B. E. H.
Affiliations
Australian National University (AU)
Venue
Journal of General Virology
Published
1986-08-01
Volume
67
Issue
8
Pages
1591-1600
ISSN
  • 0022-1317 (ISSN-L)
  • 1465-2099
OpenAlex
W2106924260
Cited by
51
References
31
Citations by year
2024: 1 · 2022: 2 · 2014: 1
FWCI
1.00
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of General Virology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Virology › Poxvirus research and outbreaks
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
  • Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
Keywords
fowlpox virus, thymidine kinase gene, vaccinia virus, gene cloning, marker rescue, viral promoters, recombinant vaccinia virus
MeSH terms
Cloning, Molecular, DNA Restriction Enzymes, Fowlpox virus, Genes, Viral, Poxviridae, Promoter Regions, Genetic, Thymidine Kinase, Vaccinia virus

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review