The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Glycoprotein C-independent binding of herpes simplex virus to cells requires cell surface heparan sulphate and glycoprotein B
Abstract
Previous studies have shown that the initial interaction of herpes simplex virus (HSV) with cells is binding to heparan sulphate and that HSV-1 glycoprotein C (gC) is principally responsible for this binding. Although gC-negative viral mutants are impaired for binding and entry, they retain significant infectivity. The purpose of the studies reported here was to explore the requirements for infectivity of gC-negative HSV-1 mutants. We found that absence or alteration of cell surface heparan sulphate significantly reduced the binding of gC-negative mutant virus and rendered cells resistant to infection, as shown previously for the wild-type virus. We isolated a recombinant double-mutated HSV strain that produces virions devoid of both of the known heparin-binding glycoproteins, gB and gC. The drastically impaired binding of these mutant virions to cells, relative to gC-negative and wild-type virions, indicates that gB mediates the binding of gC-negative virions to cells. Thus at least two HSV glycoproteins can independently mediate the binding of HSV to cell surface heparan sulphate to start the process of viral entry into cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Towbin, H., Staehelin, T., & Gordon, J. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.9.4350 · Cited by 53,385
A new technique for the assay of infectivity of human adenovirus 5 DNA
Graham, F. L., & van der Eb, A. J. (1973) · Virology · doi:10.1016/0042-6822(73)90341-3 · Cited by 9,451
Requirement of Heparan Sulfate for bFGF-Mediated Fibroblast Growth and Myoblast Differentiation
Rapraeger, A. C., Krufka, A., & Olwin, B. B. (1991) · Science · doi:10.1126/science.1646484 · Cited by 1,508
Initial interaction of herpes simplex virus with cells is binding to heparan sulfate
WuDunn, D., & Spear, P. G. (1989) · Journal of Virology · doi:10.1128/jvi.63.1.52-58.1989 · Cited by 919
Self-citationHerold, B. C., WuDunn, D., Soltys, N., & Spear, P. G. (1991) · Journal of Virology · doi:10.1128/jvi.65.3.1090-1098.1991 · Cited by 572
Self-citationCell surface receptors for herpes simplex virus are heparan sulfate proteoglycans.
Shieh, M. T., WuDunn, D., Montgomery, R. I., Esko, J. D., & Spear, P. G. (1992) · The Journal of cell biology · doi:10.1083/jcb.116.5.1273 · Cited by 545
Self-citationBaba, M., Snoeck, R., Pauwels, R., & de Clercq, E. (1988) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.32.11.1742 · Cited by 544
Animal cell mutants defective in glycosaminoglycan biosynthesis.
Esko, J. D., Stewart, T. E., & Taylor, W. H. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.10.3197 · Cited by 513
Ligas, M. W., & Johnson, D. C. (1988) · Journal of Virology · doi:10.1128/jvi.62.5.1486-1494.1988 · Cited by 510
Cited by
VIRUS-CELL AND CELL-CELL FUSION
Hernandez, L. D., Hoffman, L. R., Wolfsberg, T. G., & White, J. M. (1996) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.12.1.627 · Cited by 567
Microbial adherence to and invasion through proteoglycans
Rostand, K. S., & Esko, J. D. (1997) · Infection and Immunity · doi:10.1128/iai.65.1.1-8.1997 · Cited by 490
Herpes Simplex Virus Glycoprotein D Bound to the Human Receptor HveA
Carfı́, A., Willis, S. H., Whitbeck, J. C., Krummenacher, C., Cohen, G. H., Eisenberg, R. J., & Wiley, D. C. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00298-2 · Cited by 391
Ghosh, T., Chattopadhyay, K., Marschall, M., Karmakar, P., Mandal, P., & Ray, B. (2009) · Glycobiology · doi:10.1093/glycob/cwn092 · Cited by 391
Entry of herpesviruses into mammalian cells
Heldwein, E. E., & Krummenacher, C. (2008) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-008-7570-z · Cited by 355
Metadata
- Authors
- Herold, B. C., Visalli, R. J., Susmarski, N., Brandt, C. R., & Spear, P. G.
- Venue
- Journal of General Virology
- Publisher
- Microbiology Society
- Published
- 1994-06-01
- Volume
- 75
- Issue
- 6
- Pages
- 1211-1222
- ISSN
- 0022-1317 (ISSN-L)
- 1465-2099
- OpenAlex
- W2143783595
- Cited by
- 339
- References
- 47
- FWCI
- 13.78
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Glycoprotein C-independent binding of herpes simplex virus to cells requires cell surface heparan sulphate and glycoprotein B. https://app.cochat.ai/papers/10.1099/0022-1317-75-6-1211
- The paper
- Herold, B. C., Visalli, R. J., Susmarski, N., Brandt, C. R., & Spear, P. G. (1994). Glycoprotein C-independent binding of herpes simplex virus to cells requires cell surface heparan sulphate and glycoprotein B. Journal of General Virology. https://doi.org/10.1099/0022-1317-75-6-1211
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
