The DOI matches its Crossref record.
Detection of E5 oncoprotein in human papillomavirus type 16-positive cervical scrapes using antibodies raised to synthetic peptides
Abstract
Polyclonal antibodies were raised to partial and full-length synthetic peptides of human papillomavirus type 16 (HPV-16) E5. Antisera specificity for HPV-16 E5 was demonstrated by their ability to recognize not only their peptide immunogens but also full-length peptide and a glutathione S-transferase-E5 fusion protein. The most reactive antiserum, PE-6, raised to a full-length peptide, was used in Western blot analysis to identify HPV-16 E5 protein from exfoliated cervical cells. A strong, single band at approximately 20K was detected in two of six HPV-16-positive samples from women with a history of low-grade cervical intraepithelial neoplasia. The apparent M(r) by SDS-PAGE suggests that HPV-16 E5 forms homodimers in vivo, but not through cysteine linkage.
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“in the case of HPV-16-positive cervical and oropharyngeal cancers, the expression of E5 is more likely and has been shown to be detectable [111,112,113,114,115], suggesting that E5 may contribute to malignant progression of the cancer”
From The Not-So-Good, the Bad and the Ugly: HPV E5, E6 and E7 Oncoproteins in the Orchestration of Carcinogenesis (2021) · cites this paper as [112]
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Cited by
Basukala, O., & Banks, L. (2021) · Viruses · doi:10.3390/v13101892 · Cited by 137
Metadata
- Authors
- Kell, B., Jewers, R. J., Cason, J., Pakarian, F., Kaye, J. N., & Best, J. M.
- Venue
- Journal of General Virology
- Publisher
- Microbiology Society
- Published
- 1994-09-01
- Volume
- 75
- Issue
- 9
- Pages
- 2451-2456
- ISSN
- 0022-1317
- 1465-2099
- Cited by
- 42
- References
- 0
- Language
- en
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