The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein
Abstract
Porcine circovirus 2 (PCV2), a single-stranded DNA virus associated with post-weaning multisystemic wasting syndrome of swine, has two potential open reading frames, ORF1 and ORF2, greater than 600 nucleotides in length. ORF1 is predicted to encode a replication-associated protein (Rep) essential for replication of viral DNA, while ORF2 contains a conserved basic amino acid sequence at the N terminus resembling that of the major structural protein of chicken anaemia virus. Thus far, the structural protein(s) of PCV2 have not been identified. In this study, a viral structural protein of 30 kDa was identified in purified PCV2 particles. ORF2 of PCV2 was cloned into a baculovirus expression vector and the gene product was expressed in insect cells. The expressed ORF2 gene product had a molecular mass of 30 kDa, similar to that detected in purified virus particles. The recombinant ORF2 protein self-assembled to form capsid-like particles when viewed by electron microscopy. Antibodies against the ORF2 protein were detected in samples of sera obtained from pigs as early as 3 weeks after experimental infection with PCV2. These results show that the major structural protein of PCV2 is encoded by ORF2 and has a molecular mass of 30 kDa.
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Cites
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Metadata
- Authors
- Nawagitgul, P., Morozov, I., Bolin, S. R., Harms, P. A., Sorden, S. D., & Paul, P. S.
- Venue
- Journal of General Virology
- Publisher
- Microbiology Society
- Published
- 2000-09-01
- Volume
- 81
- Issue
- 9
- Pages
- 2281-2287
- ISSN
- 0022-1317 (ISSN-L)
- 1465-2099
- OpenAlex
- W2153314219
- Cited by
- 521
- References
- 15
- FWCI
- 16.25
- Type
- journal article
- Language
- en
- Open access
- closed
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- Nawagitgul, P., Morozov, I., Bolin, S. R., Harms, P. A., Sorden, S. D., & Paul, P. S. (2000). Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein. Journal of General Virology. https://doi.org/10.1099/0022-1317-81-9-2281
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