Cloning, sequencing and expression of the gene encoding the cell-envelope-associated proteinase from Lactobacillus paracasei subsp. paracasei NCDO 151
Abstract
The gene encoding the cell-envelope-associated proteinase of Lactobacillus paracasei subsp. paracasei NCDO 151 (formerly Lactobacillus casei NCDO 151) was cloned and sequenced. The gene was located on the chromosome and encoded a polypeptide of 1902 amino acids. The proteinase is N-terminally cleaved upon maturation. It shows extensive homology to the Lactococcus lactis subsp. cremoris Wg2 proteinase. Similar to the situation in Lactococcus, a maturation gene was found upstream of the proteinase gene. The cloned proteinase gene was expressed in Lactobacillus plantarum. However, no expression was observed when the gene was cloned in Lactococcus lactis.
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Four different types of genes encoding CSPs of dairy lactic acid bacteria have been cloned and sequenced: prtB from L. bulgaricus, prtP from Lactococcus lactis and Lactobacillus casei, prtS from Streptococcus thermophilus, and prtH from Lactobacillus helveticus.
“So far, four different types of genes encoding CSPs of dairy lactic acid bacteria have been cloned and sequenced: prtB from L. bulgaricus, prtP from Lactococcus lactis and Lactobacillus casei, prtS from Streptococcus thermophilus, and prtH from Lactobacillus helveticus (8, 13, 18, 21, 28, 38).”
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- Authors
- HOLCK, A., & NAeS, H.
- ORCID
- HOLCK, A.
- Venue
- Journal of General Microbiology
- Publisher
- Microbiology Society
- Published
- 1992-07-01
- Volume
- 138
- Issue
- 7
- Pages
- 1353-1364
- ISSN
- 0022-1287 (ISSN-L)
- OpenAlex
- W1998963977
- Cited by
- 106
- References
- 33
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 2 · 2021: 2 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 5 · 2013: 1 · 2012: 7
- FWCI
- 14.83
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of General Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Lacticaseibacillus paracasei subsp. paracasei, gene sequencing, N-terminal cleavage, Lactococcus lactis, bacterial cell envelope
- MeSH terms
- Amino Acid Sequence, Bacterial Proteins, Base Sequence, Cell Membrane, Cloning, Molecular, DNA, Bacterial, Genes, Bacterial, Lactobacillus, Metalloendopeptidases, Molecular Sequence Data, Endopeptidases, Sequence Homology, Nucleic Acid, Blotting, Southern, Restriction Mapping
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