Cited by 106Probiotics and Fermented FoodsEnzyme Production and CharacterizationBacterial Genetics and Biotechnology

Cloning, sequencing and expression of the gene encoding the cell-envelope-associated proteinase from Lactobacillus paracasei subsp. paracasei NCDO 151

HOLCK, A., NAeS, H.·1992·Journal of General Microbiology

Abstract

The gene encoding the cell-envelope-associated proteinase of Lactobacillus paracasei subsp. paracasei NCDO 151 (formerly Lactobacillus casei NCDO 151) was cloned and sequenced. The gene was located on the chromosome and encoded a polypeptide of 1902 amino acids. The proteinase is N-terminally cleaved upon maturation. It shows extensive homology to the Lactococcus lactis subsp. cremoris Wg2 proteinase. Similar to the situation in Lactococcus, a maturation gene was found upstream of the proteinase gene. The cloned proteinase gene was expressed in Lactobacillus plantarum. However, no expression was observed when the gene was cloned in Lactococcus lactis.

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Crossref · 10.1099/00221287-138-7-1353

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Authors
HOLCK, A., & NAeS, H.
Venue
Journal of General Microbiology
Published
1992-07-01
Volume
138
Issue
7
Pages
1353-1364
ISSN
0022-1287 (ISSN-L)
OpenAlex
W1998963977
Cited by
106
References
33
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2023: 2 · 2021: 2 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 5 · 2013: 1 · 2012: 7
FWCI
14.83
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of General Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Lacticaseibacillus paracasei subsp. paracasei, gene sequencing, N-terminal cleavage, Lactococcus lactis, bacterial cell envelope
MeSH terms
Amino Acid Sequence, Bacterial Proteins, Base Sequence, Cell Membrane, Cloning, Molecular, DNA, Bacterial, Genes, Bacterial, Lactobacillus, Metalloendopeptidases, Molecular Sequence Data, Endopeptidases, Sequence Homology, Nucleic Acid, Blotting, Southern, Restriction Mapping

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