The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria The GenBank accession numbers for the sequences reported in this paper are AF327711, AF327712, AF327713, AF327714, AF327715, AF327716, AF327717, AF327718, AF327719 and AF327720.
Abstract
Full exploitation of the information available in bacterial genome sequences requires the availability of facile tools for rapid genetic manipulation. One bacterium for which new genetic tools are needed is the methylotroph Methylobacterium extorquens AM1. IncQ and small IncP vectors were shown to be unsuitable for use in this bacterium, but a spontaneous mutant of a small IncP plasmid was isolated that functioned efficiently in M. extorquens AM1. This plasmid was sequenced and used as a base for developing improved broad-host-range cloning vectors. These vectors were found to replicate in a wide variety of bacterial species and have the following advantages: (1) high copy number in Escherichia coli; (2) small size (7.2 and 8.0 kb); (3) complete sequences; (4) variety of unique restriction sites; (5) blue-white screening via lacZalpha; (6) conjugative mobilization between bacterial species; and (7) readily adaptable into species-specific promoter-probe and expression vectors. Two low-background promoter-probe vectors were constructed based on these cloning vectors with either lacZ or xylE as reporter genes; these were shown to report gene expression effectively in M. extorquens AM1. Specific expression vectors were developed for use in M. extorquens AM1, which were shown to express foreign genes at significant levels, and a simple strategy is outlined to develop specific expression vectors for other bacteria. The strong mxaF promoter was used for expression, since E. coli lac-derived promoters were expressed at very low levels. This suite of genetic tools will enable a more sophisticated analysis of the physiology of M. extorquens AM1, and these vectors should also be valuable tools in the study of a variety of bacterial species.
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Cites
Molecular cloning: A laboratory manual
Deininger, P. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,682
Experiments in molecular genetics
Miller, J. H. (1972) · Medical Entomology and Zoology · Cited by 27,157
Yanisch-Perron, C., Vieira, J., & Messing, J. (1985) · Gene · doi:10.1016/0378-1119(85)90120-9 · Cited by 15,041
Vieira, J., & Messing, J. (1982) · Gene · doi:10.1016/0378-1119(82)90015-4 · Cited by 6,056
The COG database: a tool for genome-scale analysis of protein functions and evolution
Tatusov, R. L. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.1.33 · Cited by 4,520
Kovach, M. E., Elzer, P. H., Steven Hill, D., Robertson, G. T., Farris, M. A., Roop, R. M., & Peterson, K. M. (1995) · Gene · doi:10.1016/0378-1119(95)00584-1 · Cited by 3,350
Figurski, D. H., & Helinski, D. R. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.4.1648 · Cited by 3,263
Hanson, R. S., & Hanson, T. E. (1996) · Microbiological reviews · doi:10.1128/mmbr.60.2.439-471.1996 · Cited by 2,772
Ditta, G., Schmidhauser, T., Yakobson, E., Lu, P., Liang, X.-W., Finlay, D. R., Guiney, D., & Helinski, D. R. (1985) · Plasmid · doi:10.1016/0147-619x(85)90068-x · Cited by 778
A vector for systematic gene inactivation in Bacillus subtilis
Vagner, V., Dervyn, E., & Ehrlich, S. D. (1998) · Microbiology · doi:10.1099/00221287-144-11-3097 · Cited by 646
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Hickman, J. W., Tifrea, D. F., & Harwood, C. S. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0507170102 · Cited by 836
Mehta, T., Coppi, M. V., Childers, S. E., & Lovley, D. R. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.12.8634-8641.2005 · Cited by 494
Bioconversion of natural gas to liquid fuel: Opportunities and challenges
Fei, Q., Guarnieri, M. T., Tao, L., Laurens, L. M. L., Dowe, N., & Pienkos, P. T. (2014) · Biotechnology Advances · doi:10.1016/j.biotechadv.2014.03.011 · Cited by 305
The Enigmatic Planctomycetes May Hold a Key to the Origins of Methanogenesis and Methylotrophy
Chistoserdova, L. (2004) · Molecular Biology and Evolution · doi:10.1093/molbev/msh113 · Cited by 126
Kalyuzhnaya, M. G., Korotkova, N., Crowther, G., Marx, C. J., Lidstrom, M. E., & Chistoserdova, L. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.13.4607-4614.2005 · Cited by 53
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Metadata
- Authors
- Marx, C. J., & Lidstrom, M. E.
- Venue
- Microbiology
- Publisher
- Microbiology Society
- Published
- 2001-08-01
- Volume
- 147
- Issue
- 8
- Pages
- 2065-2075
- ISSN
- 1350-0872 (ISSN-L)
- 1465-2080
- OpenAlex
- W2163023998
- Cited by
- 334
- References
- 25
- FWCI
- 5.00
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria The GenBank accession numbers for the sequences reported in this paper are AF327711, AF327712, AF327713, AF327714, AF327715, AF327716, AF327717, AF327718, AF327719 and AF327720. https://app.cochat.ai/papers/10.1099/00221287-147-8-2065
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- Marx, C. J., & Lidstrom, M. E. (2001). Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria The GenBank accession numbers for the sequences reported in this paper are AF327711, AF327712, AF327713, AF327714, AF327715, AF327716, AF327717, AF327718, AF327719 and AF327720. Microbiology. https://doi.org/10.1099/00221287-147-8-2065
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